MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants.
A recently introduced Multilocus Sequence Typing scheme for Treponema pallidum subsp. pallidum was applied to clinical samples collected from 2004 to 2017 from the two largest cities (Prague and Brno) in the Czech Republic. Altogether, a total of 675 samples were tested in this study and 281 of them...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0217611 |
| _version_ | 1819146287436529664 |
|---|---|
| author | Eliška Vrbová Linda Grillová Lenka Mikalová Petra Pospíšilová Radim Strnadel Eliška Dastychová Martina Kojanová Miluše Kreidlová Daniela Vaňousová Filip Rob Přemysl Procházka Alena Krchňáková Vladimír Vašků Vladana Woznicová Monika Dvořáková Heroldová Ivana Kuklová Hana Zákoucká David Šmajs |
| author_facet | Eliška Vrbová Linda Grillová Lenka Mikalová Petra Pospíšilová Radim Strnadel Eliška Dastychová Martina Kojanová Miluše Kreidlová Daniela Vaňousová Filip Rob Přemysl Procházka Alena Krchňáková Vladimír Vašků Vladana Woznicová Monika Dvořáková Heroldová Ivana Kuklová Hana Zákoucká David Šmajs |
| author_sort | Eliška Vrbová |
| collection | DOAJ |
| description | A recently introduced Multilocus Sequence Typing scheme for Treponema pallidum subsp. pallidum was applied to clinical samples collected from 2004 to 2017 from the two largest cities (Prague and Brno) in the Czech Republic. Altogether, a total of 675 samples were tested in this study and 281 of them were found PCR-positive for treponemal DNA and typeable. Most of the typed samples (n = 281) were swabs from primary or secondary syphilis lesions (n = 231), and only a minority were whole blood or tissue samples (n = 50). Swab samples from patients with rapid plasma regain (RPR) values of 1-1024 were more frequently PCR-positive (84.6%) compared to samples from patients with non-reactive RPR test (46.5%; p-value = 0.0001). Out of 281 typeable samples, 136 were fully-typed at all TP0136, TP0548, and TP0705 loci. Among the fully and partially typed samples, 25 different allelic profiles were identified. Altogether, eight novel allelic variants were found among fully (n = 5) and partially (n = 3) typed samples. The distribution of TPA allelic profiles identified in the Czech Republic from 2004 to 2017 revealed a dynamic character with allelic profiles disappearing and emerging over time. While the number of samples with the A2058G mutation was seen to increase (86.7% in 2016/2017), the number of samples harboring the A2059G mutation was found to have decreased over time (3.3% in 2016/2017). In addition, we found several allelic profile associations with macrolide resistance or susceptibility, the gender of patients, as well as patient residence. |
| first_indexed | 2024-12-22T13:11:32Z |
| format | Article |
| id | doaj.art-b72f6a928fc24bc9bcfe0916ee670836 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T13:11:32Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b72f6a928fc24bc9bcfe0916ee6708362022-12-21T18:24:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021761110.1371/journal.pone.0217611MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants.Eliška VrbováLinda GrillováLenka MikalováPetra PospíšilováRadim StrnadelEliška DastychováMartina KojanováMiluše KreidlováDaniela VaňousováFilip RobPřemysl ProcházkaAlena KrchňákováVladimír VaškůVladana WoznicováMonika Dvořáková HeroldováIvana KuklováHana ZákouckáDavid ŠmajsA recently introduced Multilocus Sequence Typing scheme for Treponema pallidum subsp. pallidum was applied to clinical samples collected from 2004 to 2017 from the two largest cities (Prague and Brno) in the Czech Republic. Altogether, a total of 675 samples were tested in this study and 281 of them were found PCR-positive for treponemal DNA and typeable. Most of the typed samples (n = 281) were swabs from primary or secondary syphilis lesions (n = 231), and only a minority were whole blood or tissue samples (n = 50). Swab samples from patients with rapid plasma regain (RPR) values of 1-1024 were more frequently PCR-positive (84.6%) compared to samples from patients with non-reactive RPR test (46.5%; p-value = 0.0001). Out of 281 typeable samples, 136 were fully-typed at all TP0136, TP0548, and TP0705 loci. Among the fully and partially typed samples, 25 different allelic profiles were identified. Altogether, eight novel allelic variants were found among fully (n = 5) and partially (n = 3) typed samples. The distribution of TPA allelic profiles identified in the Czech Republic from 2004 to 2017 revealed a dynamic character with allelic profiles disappearing and emerging over time. While the number of samples with the A2058G mutation was seen to increase (86.7% in 2016/2017), the number of samples harboring the A2059G mutation was found to have decreased over time (3.3% in 2016/2017). In addition, we found several allelic profile associations with macrolide resistance or susceptibility, the gender of patients, as well as patient residence.https://doi.org/10.1371/journal.pone.0217611 |
| spellingShingle | Eliška Vrbová Linda Grillová Lenka Mikalová Petra Pospíšilová Radim Strnadel Eliška Dastychová Martina Kojanová Miluše Kreidlová Daniela Vaňousová Filip Rob Přemysl Procházka Alena Krchňáková Vladimír Vašků Vladana Woznicová Monika Dvořáková Heroldová Ivana Kuklová Hana Zákoucká David Šmajs MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. PLoS ONE |
| title | MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. |
| title_full | MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. |
| title_fullStr | MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. |
| title_full_unstemmed | MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. |
| title_short | MLST typing of Treponema pallidum subsp. pallidum in the Czech Republic during 2004-2017: Clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants. |
| title_sort | mlst typing of treponema pallidum subsp pallidum in the czech republic during 2004 2017 clinical isolates belonged to 25 allelic profiles and harbored 8 novel allelic variants |
| url | https://doi.org/10.1371/journal.pone.0217611 |
| work_keys_str_mv | AT eliskavrbova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT lindagrillova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT lenkamikalova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT petrapospisilova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT radimstrnadel mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT eliskadastychova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT martinakojanova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT milusekreidlova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT danielavanousova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT filiprob mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT premyslprochazka mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT alenakrchnakova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT vladimirvasku mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT vladanawoznicova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT monikadvorakovaheroldova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT ivanakuklova mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT hanazakoucka mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants AT davidsmajs mlsttypingoftreponemapallidumsubsppallidumintheczechrepublicduring20042017clinicalisolatesbelongedto25allelicprofilesandharbored8novelallelicvariants |