Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis
Free living, cosmopolitan amoebae from <i>Acanthamoeba</i> genus present a serious risk to human health. As facultative human parasites, these amoebae may cause <i>Acanthamoeba</i> keratitis (AK). <i>Acanthamoeba</i> keratitis is a severe, vision-threatening corne...
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MDPI AG
2020-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/5/350 |
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| author | Edyta B. Hendiger Marcin Padzik Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Aitor Rizo-Liendo Carlos J. Bethencourt-Estrella Desirée San Nicolás-Hernández Olfa Chiboub Rubén L. Rodríguez-Expósito Marta Grodzik Anna Pietruczuk-Padzik Karolina Stępień Gabriela Olędzka Lidia Chomicz José E. Piñero Jacob Lorenzo-Morales |
| author_facet | Edyta B. Hendiger Marcin Padzik Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Aitor Rizo-Liendo Carlos J. Bethencourt-Estrella Desirée San Nicolás-Hernández Olfa Chiboub Rubén L. Rodríguez-Expósito Marta Grodzik Anna Pietruczuk-Padzik Karolina Stępień Gabriela Olędzka Lidia Chomicz José E. Piñero Jacob Lorenzo-Morales |
| author_sort | Edyta B. Hendiger |
| collection | DOAJ |
| description | Free living, cosmopolitan amoebae from <i>Acanthamoeba</i> genus present a serious risk to human health. As facultative human parasites, these amoebae may cause <i>Acanthamoeba</i> keratitis (AK). <i>Acanthamoeba</i> keratitis is a severe, vision-threatening corneal infection with non-specific symptoms. The number of reported AK cases worldwide has been increasing every year. Moreover, 90% of <i>Acanthamoeba</i> keratitis cases are related to contact lens use. Wearing and storage contact lenses not in accordance with the physicians and manufacturers recommendations are the primary key risk factors of this disease. Amoebae can easily adhere to the contact lens surface and transmit to the corneal epithelium. Preventing amoebae adhesion to the contact lens surface could significantly decrease the number of AK infections. Until now, the effective therapy against AK is still under development. Currently proposed therapies are mainly limited to the chlorhexidine digluconate combined with propamidine isethionate or hexamidine applications, which are insufficient and very toxic to the eye. Due to lack of effective treatment, looking for new potential preventive agents is crucial to decrease the number of <i>Acanthamoeba</i> keratitis infections, especially among contact lens users. Nanoparticles have been already included in several novel therapies against bacteria, viruses, fungi, and protist. However, their anti-amoebic potential has not been fully tested yet. The aim of this study was to assess silver nanoparticles (AgNPs) and platinum nanoparticles (PtNPs) anti-amoebic activity and influence on the amoebae adhesion to the surface of four different groups of contact lenses—classified according to the Food and Drugs Administration (FDA) guidelines. The obtained results show that both tested nanoparticles were effective against <i>Acanthamoeba</i> trophozoites and decreased the amoebae adhesion to the contact lens surface. AgNPs showed better anti-amoebic activity to cytotoxicity dependence and reduced amoebae adhesion in a wider spectrum of the tested contact lenses. Our studies also confirmed that ionization next to hydration of the contact lens material is a crucial parameter influencing the <i>Acanthamoeba</i> adhesion to the contact lens surface. In conclusion, silver nanoparticles might be considered as a novel preventive agent against <i>Acanthamoeba</i> keratitis infection. |
| first_indexed | 2024-03-10T20:01:46Z |
| format | Article |
| id | doaj.art-b731b01afbc0407fb1f535f18deced39 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T20:01:46Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-b731b01afbc0407fb1f535f18deced392023-11-19T23:33:17ZengMDPI AGPathogens2076-08172020-05-019535010.3390/pathogens9050350Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba KeratitisEdyta B. Hendiger0Marcin Padzik1Ines Sifaoui2María Reyes-Batlle3Atteneri López-Arencibia4Aitor Rizo-Liendo5Carlos J. Bethencourt-Estrella6Desirée San Nicolás-Hernández7Olfa Chiboub8Rubén L. Rodríguez-Expósito9Marta Grodzik10Anna Pietruczuk-Padzik11Karolina Stępień12Gabriela Olędzka13Lidia Chomicz14José E. Piñero15Jacob Lorenzo-Morales16Instituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainDepartment of Nanobiotechnology and Experimental Ecology, Institute of Biology, Warsaw University of Life Sciences, 02-787 Warsaw, PolandDepartment of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT), Faculty of Pharmacy, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, PolandDepartment of Pharmaceutical Microbiology, Centre for Preclinical Research and Technology (CePT), Faculty of Pharmacy, Medical University of Warsaw, Banacha 1B, 02-097 Warsaw, PolandDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandDepartment of Medical Biology, Medical University of Warsaw, Litewska 14/16, 00-575 Warsaw, PolandInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainInstituto Universitario de Enfermedades Tropicales y Salud Pública de Canarias and Departamento de Obstetricia, Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna. Av. Astrofísico Francisco Sánchez S/N, 38203 Tenerife, SpainFree living, cosmopolitan amoebae from <i>Acanthamoeba</i> genus present a serious risk to human health. As facultative human parasites, these amoebae may cause <i>Acanthamoeba</i> keratitis (AK). <i>Acanthamoeba</i> keratitis is a severe, vision-threatening corneal infection with non-specific symptoms. The number of reported AK cases worldwide has been increasing every year. Moreover, 90% of <i>Acanthamoeba</i> keratitis cases are related to contact lens use. Wearing and storage contact lenses not in accordance with the physicians and manufacturers recommendations are the primary key risk factors of this disease. Amoebae can easily adhere to the contact lens surface and transmit to the corneal epithelium. Preventing amoebae adhesion to the contact lens surface could significantly decrease the number of AK infections. Until now, the effective therapy against AK is still under development. Currently proposed therapies are mainly limited to the chlorhexidine digluconate combined with propamidine isethionate or hexamidine applications, which are insufficient and very toxic to the eye. Due to lack of effective treatment, looking for new potential preventive agents is crucial to decrease the number of <i>Acanthamoeba</i> keratitis infections, especially among contact lens users. Nanoparticles have been already included in several novel therapies against bacteria, viruses, fungi, and protist. However, their anti-amoebic potential has not been fully tested yet. The aim of this study was to assess silver nanoparticles (AgNPs) and platinum nanoparticles (PtNPs) anti-amoebic activity and influence on the amoebae adhesion to the surface of four different groups of contact lenses—classified according to the Food and Drugs Administration (FDA) guidelines. The obtained results show that both tested nanoparticles were effective against <i>Acanthamoeba</i> trophozoites and decreased the amoebae adhesion to the contact lens surface. AgNPs showed better anti-amoebic activity to cytotoxicity dependence and reduced amoebae adhesion in a wider spectrum of the tested contact lenses. Our studies also confirmed that ionization next to hydration of the contact lens material is a crucial parameter influencing the <i>Acanthamoeba</i> adhesion to the contact lens surface. In conclusion, silver nanoparticles might be considered as a novel preventive agent against <i>Acanthamoeba</i> keratitis infection.https://www.mdpi.com/2076-0817/9/5/350<i>Acanthamoeba</i>keratitisnanoparticlescontact lensesadhesion |
| spellingShingle | Edyta B. Hendiger Marcin Padzik Ines Sifaoui María Reyes-Batlle Atteneri López-Arencibia Aitor Rizo-Liendo Carlos J. Bethencourt-Estrella Desirée San Nicolás-Hernández Olfa Chiboub Rubén L. Rodríguez-Expósito Marta Grodzik Anna Pietruczuk-Padzik Karolina Stępień Gabriela Olędzka Lidia Chomicz José E. Piñero Jacob Lorenzo-Morales Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis Pathogens <i>Acanthamoeba</i> keratitis nanoparticles contact lenses adhesion |
| title | Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis |
| title_full | Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis |
| title_fullStr | Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis |
| title_full_unstemmed | Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis |
| title_short | Silver Nanoparticles as a Novel Potential Preventive Agent against Acanthamoeba Keratitis |
| title_sort | silver nanoparticles as a novel potential preventive agent against acanthamoeba keratitis |
| topic | <i>Acanthamoeba</i> keratitis nanoparticles contact lenses adhesion |
| url | https://www.mdpi.com/2076-0817/9/5/350 |
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