Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays
Two main stem cell pools exist in the postnatal mammalian brain that, although they share some “stemness” properties, also exhibit significant differences. Multipotent neural stem cells survive within specialized microenvironments, called niches, and they are vulnerable to ageing. Oligodendroglial l...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/11/1743 |
| _version_ | 1797493871239757824 |
|---|---|
| author | Maria Anesti Stavroula Magkafa Efstathia Prantikou Ilias Kazanis |
| author_facet | Maria Anesti Stavroula Magkafa Efstathia Prantikou Ilias Kazanis |
| author_sort | Maria Anesti |
| collection | DOAJ |
| description | Two main stem cell pools exist in the postnatal mammalian brain that, although they share some “stemness” properties, also exhibit significant differences. Multipotent neural stem cells survive within specialized microenvironments, called niches, and they are vulnerable to ageing. Oligodendroglial lineage-restricted progenitor cells are widely distributed in the brain parenchyma and are more resistant to the effects of ageing. Here, we create polymorphic neural stem cell cultures and allow cells to progress towards the neuronal and the oligodendroglial lineage. We show that the divergence of cell fate is accompanied by a divergence in the properties of progenitors, which reflects their adaptation to life in the niche or the parenchyma. Neurogenesis shows significant spatial restrictions and a dependence on laminin, a major niche component, while oligodendrogenesis shows none of these constraints. Furthermore, the blocking of integrin-β1 leads to opposing effects, reducing neurogenesis and enhancing oligodendrogenesis. Therefore, polymorphic neural stem cell assays can be used to investigate the divergence of postnatal brain stem cells and also to predict the in vivo effects of potential therapeutic molecules targeting stem and progenitor cells, as we do for the microneurotrophin BNN-20. |
| first_indexed | 2024-03-10T01:26:11Z |
| format | Article |
| id | doaj.art-b7360f56d2604b9291d171ace11f3e74 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:26:11Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b7360f56d2604b9291d171ace11f3e742023-11-23T13:52:01ZengMDPI AGCells2073-44092022-05-011111174310.3390/cells11111743Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro AssaysMaria Anesti0Stavroula Magkafa1Efstathia Prantikou2Ilias Kazanis3Laboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, GreeceLaboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, GreeceLaboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, GreeceLaboratory of Developmental Biology, Department of Biology, University of Patras, 26504 Patras, GreeceTwo main stem cell pools exist in the postnatal mammalian brain that, although they share some “stemness” properties, also exhibit significant differences. Multipotent neural stem cells survive within specialized microenvironments, called niches, and they are vulnerable to ageing. Oligodendroglial lineage-restricted progenitor cells are widely distributed in the brain parenchyma and are more resistant to the effects of ageing. Here, we create polymorphic neural stem cell cultures and allow cells to progress towards the neuronal and the oligodendroglial lineage. We show that the divergence of cell fate is accompanied by a divergence in the properties of progenitors, which reflects their adaptation to life in the niche or the parenchyma. Neurogenesis shows significant spatial restrictions and a dependence on laminin, a major niche component, while oligodendrogenesis shows none of these constraints. Furthermore, the blocking of integrin-β1 leads to opposing effects, reducing neurogenesis and enhancing oligodendrogenesis. Therefore, polymorphic neural stem cell assays can be used to investigate the divergence of postnatal brain stem cells and also to predict the in vivo effects of potential therapeutic molecules targeting stem and progenitor cells, as we do for the microneurotrophin BNN-20.https://www.mdpi.com/2073-4409/11/11/1743neural stem cellsoligodendrocyte progenitor cellsneurogenesisoligodendrogenesiscell fatemicroenvironment |
| spellingShingle | Maria Anesti Stavroula Magkafa Efstathia Prantikou Ilias Kazanis Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays Cells neural stem cells oligodendrocyte progenitor cells neurogenesis oligodendrogenesis cell fate microenvironment |
| title | Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays |
| title_full | Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays |
| title_fullStr | Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays |
| title_full_unstemmed | Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays |
| title_short | Divergence between Neuronal and Oligodendroglial Cell Fate, in Postnatal Brain Neural Stem Cells, Leads to Divergent Properties in Polymorphic In Vitro Assays |
| title_sort | divergence between neuronal and oligodendroglial cell fate in postnatal brain neural stem cells leads to divergent properties in polymorphic in vitro assays |
| topic | neural stem cells oligodendrocyte progenitor cells neurogenesis oligodendrogenesis cell fate microenvironment |
| url | https://www.mdpi.com/2073-4409/11/11/1743 |
| work_keys_str_mv | AT mariaanesti divergencebetweenneuronalandoligodendroglialcellfateinpostnatalbrainneuralstemcellsleadstodivergentpropertiesinpolymorphicinvitroassays AT stavroulamagkafa divergencebetweenneuronalandoligodendroglialcellfateinpostnatalbrainneuralstemcellsleadstodivergentpropertiesinpolymorphicinvitroassays AT efstathiaprantikou divergencebetweenneuronalandoligodendroglialcellfateinpostnatalbrainneuralstemcellsleadstodivergentpropertiesinpolymorphicinvitroassays AT iliaskazanis divergencebetweenneuronalandoligodendroglialcellfateinpostnatalbrainneuralstemcellsleadstodivergentpropertiesinpolymorphicinvitroassays |