Challenges in Quantifying Cytosine Methylation in the HIV Provirus
ABSTRACT DNA methylation is an epigenetic mechanism most commonly associated with transcriptional repression. While it is clear that DNA methylation can silence HIV proviral expression in in vitro latency models, its correlation with HIV persistence and expression in vivo is ambiguous, particularly...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2019-02-01
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| Series: | mBio |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/mBio.02268-18 |
| _version_ | 1818740268854149120 |
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| author | Sarah A. LaMere Antoine Chaillon Christina Huynh Davey M. Smith Sara Gianella |
| author_facet | Sarah A. LaMere Antoine Chaillon Christina Huynh Davey M. Smith Sara Gianella |
| author_sort | Sarah A. LaMere |
| collection | DOAJ |
| description | ABSTRACT DNA methylation is an epigenetic mechanism most commonly associated with transcriptional repression. While it is clear that DNA methylation can silence HIV proviral expression in in vitro latency models, its correlation with HIV persistence and expression in vivo is ambiguous, particularly in persons living with HIV (PLWH) receiving antiretroviral therapy (ART). Several factors potentially contribute to discrepancies between results in the literature, including differences in integration sites, functional proviral load, sampling bias, and stochastic PCR amplification. Recent studies into genomic features of cytosine methylation sites in mammalian genes offer potentially significant insights into this mechanism. Here, we discuss the importance of these factors in the context of the HIV. |
| first_indexed | 2024-12-18T01:38:02Z |
| format | Article |
| id | doaj.art-b7390ba0885f4d55bdc9305fde4be517 |
| institution | Directory Open Access Journal |
| issn | 2150-7511 |
| language | English |
| last_indexed | 2024-12-18T01:38:02Z |
| publishDate | 2019-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mBio |
| spelling | doaj.art-b7390ba0885f4d55bdc9305fde4be5172022-12-21T21:25:24ZengAmerican Society for MicrobiologymBio2150-75112019-02-0110110.1128/mBio.02268-18Challenges in Quantifying Cytosine Methylation in the HIV ProvirusSarah A. LaMere0Antoine Chaillon1Christina Huynh2Davey M. Smith3Sara Gianella4Department of Medicine, University of California San Diego, La Jolla, California, USADepartment of Medicine, University of California San Diego, La Jolla, California, USADepartment of Medicine, University of California San Diego, La Jolla, California, USADepartment of Medicine, University of California San Diego, La Jolla, California, USADepartment of Medicine, University of California San Diego, La Jolla, California, USAABSTRACT DNA methylation is an epigenetic mechanism most commonly associated with transcriptional repression. While it is clear that DNA methylation can silence HIV proviral expression in in vitro latency models, its correlation with HIV persistence and expression in vivo is ambiguous, particularly in persons living with HIV (PLWH) receiving antiretroviral therapy (ART). Several factors potentially contribute to discrepancies between results in the literature, including differences in integration sites, functional proviral load, sampling bias, and stochastic PCR amplification. Recent studies into genomic features of cytosine methylation sites in mammalian genes offer potentially significant insights into this mechanism. Here, we discuss the importance of these factors in the context of the HIV.https://journals.asm.org/doi/10.1128/mBio.02268-18HIV latencycytosine methylationepigenetic silencingnon-CpG methylation |
| spellingShingle | Sarah A. LaMere Antoine Chaillon Christina Huynh Davey M. Smith Sara Gianella Challenges in Quantifying Cytosine Methylation in the HIV Provirus mBio HIV latency cytosine methylation epigenetic silencing non-CpG methylation |
| title | Challenges in Quantifying Cytosine Methylation in the HIV Provirus |
| title_full | Challenges in Quantifying Cytosine Methylation in the HIV Provirus |
| title_fullStr | Challenges in Quantifying Cytosine Methylation in the HIV Provirus |
| title_full_unstemmed | Challenges in Quantifying Cytosine Methylation in the HIV Provirus |
| title_short | Challenges in Quantifying Cytosine Methylation in the HIV Provirus |
| title_sort | challenges in quantifying cytosine methylation in the hiv provirus |
| topic | HIV latency cytosine methylation epigenetic silencing non-CpG methylation |
| url | https://journals.asm.org/doi/10.1128/mBio.02268-18 |
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