Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies
Chronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure usin...
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MDPI AG
2022-09-01
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| author | Lei Wang Lieke E. J. van Iersel Charlotte E. Pelgrim Jingyi Lu Ingrid van Ark Thea Leusink-Muis Harry R. Gosker Ramon C. J. Langen Annemie M. W. J. Schols Josep M. Argilés Ardy van Helvoort Aletta D. Kraneveld Johan Garssen Paul A. J. Henricks Gert Folkerts Saskia Braber |
| author_facet | Lei Wang Lieke E. J. van Iersel Charlotte E. Pelgrim Jingyi Lu Ingrid van Ark Thea Leusink-Muis Harry R. Gosker Ramon C. J. Langen Annemie M. W. J. Schols Josep M. Argilés Ardy van Helvoort Aletta D. Kraneveld Johan Garssen Paul A. J. Henricks Gert Folkerts Saskia Braber |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Chronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure using in vivo and in vitro models. Mice were exposed to cigarette smoke or air for 72 days and the pre-adipose cell line 3T3-L1 was utilized as an in vitro model. Cigarette smoke exposure decreased body weight, and the proportional loss in fat mass was more pronounced than the lean mass loss. Cigarette smoke exposure reduced adipocyte size and increased adipocyte numbers. Adipose macrophage numbers and associated cytokine levels, including interleukin-1β, interleukine-6 and tumor necrosis factor-α were elevated in smoke-exposed mice. Muscle strength and protein synthesis signaling were decreased after smoke exposure; however, muscle mass was not changed. In vitro studies demonstrated that lipolysis and fatty acid oxidation were upregulated in cigarette smoke-exposed pre-adipocytes. In conclusion, cigarette smoke exposure induces a loss of whole-body fat mass and adipose atrophy, which is likely due to enhanced lipolysis. |
| first_indexed | 2024-03-10T00:25:59Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:25:59Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Cells |
| spelling | doaj.art-b739839576774b14b32bd27d18d73e562023-11-23T15:34:00ZengMDPI AGCells2073-44092022-09-011118289310.3390/cells11182893Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro StudiesLei Wang0Lieke E. J. van Iersel1Charlotte E. Pelgrim2Jingyi Lu3Ingrid van Ark4Thea Leusink-Muis5Harry R. Gosker6Ramon C. J. Langen7Annemie M. W. J. Schols8Josep M. Argilés9Ardy van Helvoort10Aletta D. Kraneveld11Johan Garssen12Paul A. J. Henricks13Gert Folkerts14Saskia Braber15Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDepartment of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre +, 6200 MD Maastricht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDepartment of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre +, 6200 MD Maastricht, The NetherlandsDepartment of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre +, 6200 MD Maastricht, The NetherlandsDepartment of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre +, 6200 MD Maastricht, The NetherlandsBiochemistry and Molecular Biology of Cancer, Faculty of Biology, University of Barcelona, 08007 Barcelona, SpainDepartment of Respiratory Medicine, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre +, 6200 MD Maastricht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsDivision of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584 CG Utrecht, The NetherlandsChronic obstructive pulmonary disease (COPD), often caused by smoking, is a chronic lung disease with systemic manifestations including metabolic comorbidities. This study investigates adaptive and pathological alterations in adipose and skeletal muscle tissue following cigarette smoke exposure using in vivo and in vitro models. Mice were exposed to cigarette smoke or air for 72 days and the pre-adipose cell line 3T3-L1 was utilized as an in vitro model. Cigarette smoke exposure decreased body weight, and the proportional loss in fat mass was more pronounced than the lean mass loss. Cigarette smoke exposure reduced adipocyte size and increased adipocyte numbers. Adipose macrophage numbers and associated cytokine levels, including interleukin-1β, interleukine-6 and tumor necrosis factor-α were elevated in smoke-exposed mice. Muscle strength and protein synthesis signaling were decreased after smoke exposure; however, muscle mass was not changed. In vitro studies demonstrated that lipolysis and fatty acid oxidation were upregulated in cigarette smoke-exposed pre-adipocytes. In conclusion, cigarette smoke exposure induces a loss of whole-body fat mass and adipose atrophy, which is likely due to enhanced lipolysis.https://www.mdpi.com/2073-4409/11/18/2893COPDcigarette smokecachexiaadipose tissuelipolysisskeletal muscle |
| spellingShingle | Lei Wang Lieke E. J. van Iersel Charlotte E. Pelgrim Jingyi Lu Ingrid van Ark Thea Leusink-Muis Harry R. Gosker Ramon C. J. Langen Annemie M. W. J. Schols Josep M. Argilés Ardy van Helvoort Aletta D. Kraneveld Johan Garssen Paul A. J. Henricks Gert Folkerts Saskia Braber Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies Cells COPD cigarette smoke cachexia adipose tissue lipolysis skeletal muscle |
| title | Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies |
| title_full | Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies |
| title_fullStr | Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies |
| title_full_unstemmed | Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies |
| title_short | Effects of Cigarette Smoke on Adipose and Skeletal Muscle Tissue: In Vivo and In Vitro Studies |
| title_sort | effects of cigarette smoke on adipose and skeletal muscle tissue in vivo and in vitro studies |
| topic | COPD cigarette smoke cachexia adipose tissue lipolysis skeletal muscle |
| url | https://www.mdpi.com/2073-4409/11/18/2893 |
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