Modulation of Skin Inflammatory Response by Active Components of Silymarin
In this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with...
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MDPI AG
2018-12-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/24/1/123 |
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| author | Jana Juráňová Juliette Aury-Landas Karim Boumediene Catherine Baugé David Biedermann Jitka Ulrichová Jana Franková |
| author_facet | Jana Juráňová Juliette Aury-Landas Karim Boumediene Catherine Baugé David Biedermann Jitka Ulrichová Jana Franková |
| author_sort | Jana Juráňová |
| collection | DOAJ |
| description | In this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with persistent inflammation, we studied their anti-inflammatory activity against inflammation induced by bacterial lipopolysaccharide (LPS). We investigated the regulation of crucial pro-inflammatory transcription factors—nuclear factor kappa-B (NF-κB) and activator protein 1 (AP-1)—as well as the expression of downstream inflammatory targets by Western blotting, real-time PCR (RT-PCR), electrophoretic mobility shift assay (EMSA), and/or enzyme-linked immunosorbent assay (ELISA) in vitro using primary normal human dermal fibroblasts (NHDF). We demonstrated the greater ability of DHS to modulate the pro-inflammatory cytokines production via the NF-κB and AP-1 signaling pathways when compared to other tested substances. The prolonged exposure of LPS-challenged human dermal fibroblasts to DHS had both beneficial and detrimental consequences. DHS diminished interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion but induced the significant upregulation of IL-8 mRNA associated with NF-κB and AP-1 activation. The observed conflicting results may compromise the main expected benefit, which is the acceleration of the healing of the wound via a diminished inflammation. |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-13T02:06:23Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b744cf9eaa6e4b109c05b5a5ba6c63382022-12-22T03:07:27ZengMDPI AGMolecules1420-30492018-12-0124112310.3390/molecules24010123molecules24010123Modulation of Skin Inflammatory Response by Active Components of SilymarinJana Juráňová0Juliette Aury-Landas1Karim Boumediene2Catherine Baugé3David Biedermann4Jitka Ulrichová5Jana Franková6Department of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, 775 15 Olomouc, Czech RepublicEA7451 BioConnecT, Normandie University, UNICAEN, 14000 Caen, FranceEA7451 BioConnecT, Normandie University, UNICAEN, 14000 Caen, FranceEA7451 BioConnecT, Normandie University, UNICAEN, 14000 Caen, FranceInstitute of Microbiology of the Czech Academy of Sciences, Laboratory of Biotransformation, Vídeňská 1083, 14220 Praha 4, Czech RepublicDepartment of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, 775 15 Olomouc, Czech RepublicDepartment of Medical Chemistry and Biochemistry, Faculty of Medicine and Dentistry, Palacky University, Hněvotínská 3, 775 15 Olomouc, Czech RepublicIn this study, we compared selected silymarin components, such as quercetin (QE), 2,3-dehydrosilybin (DHS) and silybin (SB), with the anti-inflammatory drug indomethacin (IND) in terms of their wound healing potential. In view of the fact that pathological cutaneous wound healing is associated with persistent inflammation, we studied their anti-inflammatory activity against inflammation induced by bacterial lipopolysaccharide (LPS). We investigated the regulation of crucial pro-inflammatory transcription factors—nuclear factor kappa-B (NF-κB) and activator protein 1 (AP-1)—as well as the expression of downstream inflammatory targets by Western blotting, real-time PCR (RT-PCR), electrophoretic mobility shift assay (EMSA), and/or enzyme-linked immunosorbent assay (ELISA) in vitro using primary normal human dermal fibroblasts (NHDF). We demonstrated the greater ability of DHS to modulate the pro-inflammatory cytokines production via the NF-κB and AP-1 signaling pathways when compared to other tested substances. The prolonged exposure of LPS-challenged human dermal fibroblasts to DHS had both beneficial and detrimental consequences. DHS diminished interleukin-6 (IL-6) and interleukin-8 (IL-8) secretion but induced the significant upregulation of IL-8 mRNA associated with NF-κB and AP-1 activation. The observed conflicting results may compromise the main expected benefit, which is the acceleration of the healing of the wound via a diminished inflammation.http://www.mdpi.com/1420-3049/24/1/123fibroblastsinflammationskin wound healingcytokinesNF-κB |
| spellingShingle | Jana Juráňová Juliette Aury-Landas Karim Boumediene Catherine Baugé David Biedermann Jitka Ulrichová Jana Franková Modulation of Skin Inflammatory Response by Active Components of Silymarin Molecules fibroblasts inflammation skin wound healing cytokines NF-κB |
| title | Modulation of Skin Inflammatory Response by Active Components of Silymarin |
| title_full | Modulation of Skin Inflammatory Response by Active Components of Silymarin |
| title_fullStr | Modulation of Skin Inflammatory Response by Active Components of Silymarin |
| title_full_unstemmed | Modulation of Skin Inflammatory Response by Active Components of Silymarin |
| title_short | Modulation of Skin Inflammatory Response by Active Components of Silymarin |
| title_sort | modulation of skin inflammatory response by active components of silymarin |
| topic | fibroblasts inflammation skin wound healing cytokines NF-κB |
| url | http://www.mdpi.com/1420-3049/24/1/123 |
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