Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana
Abscisic acid (ABA) hydroxylation is an important pathway for ABA inactivation and homeostasis maintenance. Here, we discover a new downstream catabolite of neophaseic acid (neoPA) in the ABA 9′-hydroxyl pathway and identify it as epi-neodihydrophaseic acid (epi-neoDPA) by comparing its accurate mas...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
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| Series: | Plant Communications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590346222000955 |
| _version_ | 1798035988802437120 |
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| author | Ya-Li Bai Xiaoming Yin Cai-Feng Xiong Bao-Dong Cai Yan Wu Xiao-Yun Zhang Zhenwei Wei Tiantian Ye Yu-Qi Feng |
| author_facet | Ya-Li Bai Xiaoming Yin Cai-Feng Xiong Bao-Dong Cai Yan Wu Xiao-Yun Zhang Zhenwei Wei Tiantian Ye Yu-Qi Feng |
| author_sort | Ya-Li Bai |
| collection | DOAJ |
| description | Abscisic acid (ABA) hydroxylation is an important pathway for ABA inactivation and homeostasis maintenance. Here, we discover a new downstream catabolite of neophaseic acid (neoPA) in the ABA 9′-hydroxyl pathway and identify it as epi-neodihydrophaseic acid (epi-neoDPA) by comparing its accurate mass, retention time, and MSn spectra with those of our chemically synthesized epi-neoDPA. Analyses of Arabidopsis seed germination and ABA-related gene expression reveal that neoPA rather than epi-neoDPA possesses ABA-like hormonal activity. In vitro enzyme activity tests of prokaryotic recombinant protein reveal that NeoPAR1 (neoPA reductase 1) identified from Arabidopsis converts neoPA into epi-neoDPA. Site-directed mutation at Tyr163 in the conserved motif of NeoPAR1 abolishes the catalytic activity of NeoPAR1. Accelerated seed germination was observed in NeoPAR1 knockdown and knockout mutants, whereas retarded seed germination and the accumulation of epi-neoDPA and ABA were observed in NeoPAR1 overexpression lines, suggesting that NeoPAR1 is involved in seed germination and maintenance of ABA homeostasis. |
| first_indexed | 2024-04-11T21:06:14Z |
| format | Article |
| id | doaj.art-b747aa33e37f4da28c2507277d3cfa03 |
| institution | Directory Open Access Journal |
| issn | 2590-3462 |
| language | English |
| last_indexed | 2024-04-11T21:06:14Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Communications |
| spelling | doaj.art-b747aa33e37f4da28c2507277d3cfa032022-12-22T04:03:21ZengElsevierPlant Communications2590-34622022-09-0135100340Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thalianaYa-Li Bai0Xiaoming Yin1Cai-Feng Xiong2Bao-Dong Cai3Yan Wu4Xiao-Yun Zhang5Zhenwei Wei6Tiantian Ye7Yu-Qi Feng8Department of Chemistry, Wuhan University, Wuhan 430072, P.R. ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, P.R. ChinaDepartment of Chemistry, Lanzhou University, Lanzhou 730000, P.R. ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. ChinaDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. China; Corresponding authorDepartment of Chemistry, Wuhan University, Wuhan 430072, P.R. China; Frontier Science Center for Immunology and Metabolism, Wuhan University, Wuhan 430072, P.R. China; Corresponding authorAbscisic acid (ABA) hydroxylation is an important pathway for ABA inactivation and homeostasis maintenance. Here, we discover a new downstream catabolite of neophaseic acid (neoPA) in the ABA 9′-hydroxyl pathway and identify it as epi-neodihydrophaseic acid (epi-neoDPA) by comparing its accurate mass, retention time, and MSn spectra with those of our chemically synthesized epi-neoDPA. Analyses of Arabidopsis seed germination and ABA-related gene expression reveal that neoPA rather than epi-neoDPA possesses ABA-like hormonal activity. In vitro enzyme activity tests of prokaryotic recombinant protein reveal that NeoPAR1 (neoPA reductase 1) identified from Arabidopsis converts neoPA into epi-neoDPA. Site-directed mutation at Tyr163 in the conserved motif of NeoPAR1 abolishes the catalytic activity of NeoPAR1. Accelerated seed germination was observed in NeoPAR1 knockdown and knockout mutants, whereas retarded seed germination and the accumulation of epi-neoDPA and ABA were observed in NeoPAR1 overexpression lines, suggesting that NeoPAR1 is involved in seed germination and maintenance of ABA homeostasis.http://www.sciencedirect.com/science/article/pii/S2590346222000955abscisic acidABA catabolismepi-neoDPAneoPA reductaseseed germinationphaseic acid |
| spellingShingle | Ya-Li Bai Xiaoming Yin Cai-Feng Xiong Bao-Dong Cai Yan Wu Xiao-Yun Zhang Zhenwei Wei Tiantian Ye Yu-Qi Feng Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana Plant Communications abscisic acid ABA catabolism epi-neoDPA neoPA reductase seed germination phaseic acid |
| title | Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana |
| title_full | Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana |
| title_fullStr | Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana |
| title_full_unstemmed | Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana |
| title_short | Neophaseic acid catabolism in the 9′-hydroxylation pathway of abscisic acid in Arabidopsis thaliana |
| title_sort | neophaseic acid catabolism in the 9 hydroxylation pathway of abscisic acid in arabidopsis thaliana |
| topic | abscisic acid ABA catabolism epi-neoDPA neoPA reductase seed germination phaseic acid |
| url | http://www.sciencedirect.com/science/article/pii/S2590346222000955 |
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