Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water
Cadmium (Cd<sup>2+</sup>) is one of the most toxic heavy metals causing serious health problems; thus, designing accurate analytical methods for monitoring such pollutants is highly urgent. Herein, we report a label-free electrochemical aptasensor for cadmium detection in water. For this...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/1/105 |
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| author | Selma Rabai Ahlem Teniou Gaëlle Catanante Messaoud Benounis Jean-Louis Marty Amina Rhouati |
| author_facet | Selma Rabai Ahlem Teniou Gaëlle Catanante Messaoud Benounis Jean-Louis Marty Amina Rhouati |
| author_sort | Selma Rabai |
| collection | DOAJ |
| description | Cadmium (Cd<sup>2+</sup>) is one of the most toxic heavy metals causing serious health problems; thus, designing accurate analytical methods for monitoring such pollutants is highly urgent. Herein, we report a label-free electrochemical aptasensor for cadmium detection in water. For this, a nanocomposite combining the advantages of gold nanoparticles (AuNPs), carbon nanotubes (CNTs) and chitosan (Cs) was constructed and used as immobilization support for the cadmium aptamer. First, the surface of a glassy carbon electrode (GCE) was modified with CNTs-CS. Then, AuNPs were deposited on CNTs-CS/GCE using chrono-amperometry. Finally, the immobilization of the amino-modified Cd-aptamer was achieved via glutaraldehyde cross-linking. The different synthesis steps of the AuNPs/CNTs/CS nano assembly were characterized by cyclic voltammetry (CV). Electrochemical impedance spectroscopy (EIS) was employed for cadmium determination. The proposed biosensor exhibited excellent performances for cadmium detection at a low applied potential (−0.5 V) with a high sensitivity (1.2 KΩ·M<sup>−1</sup>), a detection limit of 0.02 pM and a wide linear range (10<sup>−13</sup>–10<sup>−4</sup> M). Moreover, the aptasensor showed a good selectivity against the interfering ions: Pb<sup>2+</sup>; Hg<sup>2+</sup> and Zn<sup>2+</sup>. Our electrochemical biosensor provides a simple and sensitive approach for Cd<sup>2+</sup> detection in aqueous solutions, with promising applications in the monitoring of trace amounts of heavy metals in real samples. |
| first_indexed | 2024-03-10T03:22:24Z |
| format | Article |
| id | doaj.art-b74c7aaed7904380b1139b6176976cec |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-10T03:22:24Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-b74c7aaed7904380b1139b6176976cec2023-11-23T12:16:56ZengMDPI AGSensors1424-82202021-12-0122110510.3390/s22010105Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in WaterSelma Rabai0Ahlem Teniou1Gaëlle Catanante2Messaoud Benounis3Jean-Louis Marty4Amina Rhouati5Laboratory of Sensors, Instrumentations and Process (LCIP), University of Khenchela, Khenchela 40000, AlgeriaBioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, AlgeriaLaboratoire Biocapteurs, Analyse et Environnement (BAE), Université de Perpignan Via Domitia, 66860 Perpignan, FranceLaboratory of Sensors, Instrumentations and Process (LCIP), University of Khenchela, Khenchela 40000, AlgeriaBioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, AlgeriaBioengineering Laboratory, Higher National School of Biotechnology, Constantine 25100, AlgeriaCadmium (Cd<sup>2+</sup>) is one of the most toxic heavy metals causing serious health problems; thus, designing accurate analytical methods for monitoring such pollutants is highly urgent. Herein, we report a label-free electrochemical aptasensor for cadmium detection in water. For this, a nanocomposite combining the advantages of gold nanoparticles (AuNPs), carbon nanotubes (CNTs) and chitosan (Cs) was constructed and used as immobilization support for the cadmium aptamer. First, the surface of a glassy carbon electrode (GCE) was modified with CNTs-CS. Then, AuNPs were deposited on CNTs-CS/GCE using chrono-amperometry. Finally, the immobilization of the amino-modified Cd-aptamer was achieved via glutaraldehyde cross-linking. The different synthesis steps of the AuNPs/CNTs/CS nano assembly were characterized by cyclic voltammetry (CV). Electrochemical impedance spectroscopy (EIS) was employed for cadmium determination. The proposed biosensor exhibited excellent performances for cadmium detection at a low applied potential (−0.5 V) with a high sensitivity (1.2 KΩ·M<sup>−1</sup>), a detection limit of 0.02 pM and a wide linear range (10<sup>−13</sup>–10<sup>−4</sup> M). Moreover, the aptasensor showed a good selectivity against the interfering ions: Pb<sup>2+</sup>; Hg<sup>2+</sup> and Zn<sup>2+</sup>. Our electrochemical biosensor provides a simple and sensitive approach for Cd<sup>2+</sup> detection in aqueous solutions, with promising applications in the monitoring of trace amounts of heavy metals in real samples.https://www.mdpi.com/1424-8220/22/1/105cadmiumaptasensorelectrochemicalenvironmentpollutant |
| spellingShingle | Selma Rabai Ahlem Teniou Gaëlle Catanante Messaoud Benounis Jean-Louis Marty Amina Rhouati Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water Sensors cadmium aptasensor electrochemical environment pollutant |
| title | Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water |
| title_full | Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water |
| title_fullStr | Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water |
| title_full_unstemmed | Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water |
| title_short | Fabrication of AuNPs/MWCNTS/Chitosan Nanocomposite for the Electrochemical Aptasensing of Cadmium in Water |
| title_sort | fabrication of aunps mwcnts chitosan nanocomposite for the electrochemical aptasensing of cadmium in water |
| topic | cadmium aptasensor electrochemical environment pollutant |
| url | https://www.mdpi.com/1424-8220/22/1/105 |
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