Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting
Bioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability of the bioink depends on the physical properties such as viscosity. However, the high viscosity bioink puts shear stress on the cells and low viscosity...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/10/2377 |
| _version_ | 1797550816689651712 |
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| author | Wonseop Lim Gyeong Jin Kim Hyun Woo Kim Jiyeon Lee Xiaowei Zhang Min Gyeong Kang Jeong Wook Seo Jae Min Cha Hyun Jin Park Min-Young Lee Su Ryon Shin Seon Young Shin Hojae Bae |
| author_facet | Wonseop Lim Gyeong Jin Kim Hyun Woo Kim Jiyeon Lee Xiaowei Zhang Min Gyeong Kang Jeong Wook Seo Jae Min Cha Hyun Jin Park Min-Young Lee Su Ryon Shin Seon Young Shin Hojae Bae |
| author_sort | Wonseop Lim |
| collection | DOAJ |
| description | Bioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability of the bioink depends on the physical properties such as viscosity. However, the high viscosity bioink puts shear stress on the cells and low viscosity bioink cannot maintain complex tissue structure firmly after the printing. In this work, we applied dual crosslinkable bioink using Kappa-carrageenan (κ-CA) to overcome existing shortcomings. κ-CA has properties such as biocompatibility, biodegradability, shear-thinning and ionic gelation but the difficulty of controlling gelation properties makes it unsuitable for application in 3D bioprinting. This problem was solved by synthesizing methacrylated Kappa-carrageenan (MA-κ-CA), which can be dual crosslinked through ionic and UV (Ultraviolet) crosslinking to form hydrogel using NIH-3T3 cells. Through MA substitutions, the rheological properties of the gel could be controlled to reduce the shear stress. Moreover, bioprinting using the cell-laden MA-κ-CA showed cell compatibility with enhanced shape retention capability. The potential to control the physical properties through dual crosslinking of MA-κ-CA hydrogel is expected to be widely applied in 3D bioprinting applications. |
| first_indexed | 2024-03-10T15:35:49Z |
| format | Article |
| id | doaj.art-b74dcd0b99ec402890215d134de2cca6 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T15:35:49Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-b74dcd0b99ec402890215d134de2cca62023-11-20T17:16:37ZengMDPI AGPolymers2073-43602020-10-011210237710.3390/polym12102377Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type BioprintingWonseop Lim0Gyeong Jin Kim1Hyun Woo Kim2Jiyeon Lee3Xiaowei Zhang4Min Gyeong Kang5Jeong Wook Seo6Jae Min Cha7Hyun Jin Park8Min-Young Lee9Su Ryon Shin10Seon Young Shin11Hojae Bae12KU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 02841, KoreaKU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaKU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul 05029, KoreaKU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaDepartment of Mechatronics, College of Engineering, Incheon National University, Incheon 22012, KoreaDepartment of Biotechnology, College of Life Science and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul 02841, KoreaSmart Healthcare Research Institute, Biomedical Engineering Research Center, Samsung Medical Center, 81, Irwon-ro, Gangnam-gu, Seoul 06351, KoreaDivision of Engineering in Medicine, Department of Medicine, Harvard Medical School, Brigham and Women’s Hospital, Cambridge, MA 02139, USAKU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaKU Convergence Science and Technology Institute, Department of Stem Cell and Regenerative Biotechnology, Konkuk University, Seoul 05029, KoreaBioink based 3D bioprinting is a promising new technology that enables fabrication of complex tissue structures with living cells. The printability of the bioink depends on the physical properties such as viscosity. However, the high viscosity bioink puts shear stress on the cells and low viscosity bioink cannot maintain complex tissue structure firmly after the printing. In this work, we applied dual crosslinkable bioink using Kappa-carrageenan (κ-CA) to overcome existing shortcomings. κ-CA has properties such as biocompatibility, biodegradability, shear-thinning and ionic gelation but the difficulty of controlling gelation properties makes it unsuitable for application in 3D bioprinting. This problem was solved by synthesizing methacrylated Kappa-carrageenan (MA-κ-CA), which can be dual crosslinked through ionic and UV (Ultraviolet) crosslinking to form hydrogel using NIH-3T3 cells. Through MA substitutions, the rheological properties of the gel could be controlled to reduce the shear stress. Moreover, bioprinting using the cell-laden MA-κ-CA showed cell compatibility with enhanced shape retention capability. The potential to control the physical properties through dual crosslinking of MA-κ-CA hydrogel is expected to be widely applied in 3D bioprinting applications.https://www.mdpi.com/2073-4360/12/10/2377kappa-carrageenanmethacrylic anhydridedual crosslinking hydrogel3D bioprinting |
| spellingShingle | Wonseop Lim Gyeong Jin Kim Hyun Woo Kim Jiyeon Lee Xiaowei Zhang Min Gyeong Kang Jeong Wook Seo Jae Min Cha Hyun Jin Park Min-Young Lee Su Ryon Shin Seon Young Shin Hojae Bae Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting Polymers kappa-carrageenan methacrylic anhydride dual crosslinking hydrogel 3D bioprinting |
| title | Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting |
| title_full | Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting |
| title_fullStr | Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting |
| title_full_unstemmed | Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting |
| title_short | Kappa-Carrageenan-Based Dual Crosslinkable Bioink for Extrusion Type Bioprinting |
| title_sort | kappa carrageenan based dual crosslinkable bioink for extrusion type bioprinting |
| topic | kappa-carrageenan methacrylic anhydride dual crosslinking hydrogel 3D bioprinting |
| url | https://www.mdpi.com/2073-4360/12/10/2377 |
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