Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture
The use of arbuscular mycorrhizal fungi (AMF) as biofertilizer in agriculture is a sustainable approach to fertilization. The first step in the production of AMF biofertilizer is inoculation of mycotrophic plants with a composite of soil and native plant roots, containing potentially viable AMF spor...
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| フォーマット: | 論文 |
| 言語: | English |
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MDPI AG
2022-10-01
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| シリーズ: | Journal of Fungi |
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| オンライン･アクセス: | https://www.mdpi.com/2309-608X/8/10/1061 |
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| author | Ugyen Wangmo Tenzin Nuttapol Noirungsee Phanthipha Runsaeng Prakrit Noppradit Lompong Klinnawee |
| author_facet | Ugyen Wangmo Tenzin Nuttapol Noirungsee Phanthipha Runsaeng Prakrit Noppradit Lompong Klinnawee |
| author_sort | Ugyen Wangmo Tenzin |
| collection | DOAJ |
| description | The use of arbuscular mycorrhizal fungi (AMF) as biofertilizer in agriculture is a sustainable approach to fertilization. The first step in the production of AMF biofertilizer is inoculation of mycotrophic plants with a composite of soil and native plant roots, containing potentially viable AMF spores from natural habitats, to a trap culture. A single host plant or a consortium of host plants can be used to propagate AMF spores. However, the difference in the comparative efficiency of mono- and co-cultivated host plants used for the production of AMF spores and the maintenance of original AMF community composition has not been well elucidated. Here, we prepared trap culture with nutrient-poor soil from coastal sand dune vegetation collected during the dry season when the AMF spore density and relative abundance of Glomeromycota ITS2 sequences were significantly higher (<i>p</i> = <0.05) than in the wet season. The AMF communities in the soil were mainly composed of <i>Glomus</i> spp. Maize (<i>Zea mays</i> L.) and/or Sorghum (<i>Sorghum bicolor</i> (L.). Moench) were grown in trap cultures in the greenhouse. Our results demonstrated that co-cultivation of the host plants increased the production of AMF spores but, compared to mono-cultivation of host plants, did not better sustain the native AMF community compositions in the coastal sand dune soil. We propose that the co-cultivation of host plants in a trap culture broadens AMF-host plant compatibilities and thus sustains the symbiotic association of the natively diverse AMF. Therefore, the results of this study suggest that further research is needed to confirm whether the co-culturing of more than one host plant is as efficient a strategy as using a monoculture of a single host plant. |
| first_indexed | 2024-03-09T20:00:19Z |
| format | Article |
| id | doaj.art-b74f6726f5934f79b966064815287fac |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T20:00:19Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-b74f6726f5934f79b966064815287fac2023-11-24T00:47:24ZengMDPI AGJournal of Fungi2309-608X2022-10-01810106110.3390/jof8101061Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap CultureUgyen Wangmo Tenzin0Nuttapol Noirungsee1Phanthipha Runsaeng2Prakrit Noppradit3Lompong Klinnawee4Division of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDivision of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandFaculty of Environmental Management, Prince of Songkla University, Songkhla 90110, ThailandDivision of Biological Science, Faculty of Science, Prince of Songkla University, Songkhla 90110, ThailandThe use of arbuscular mycorrhizal fungi (AMF) as biofertilizer in agriculture is a sustainable approach to fertilization. The first step in the production of AMF biofertilizer is inoculation of mycotrophic plants with a composite of soil and native plant roots, containing potentially viable AMF spores from natural habitats, to a trap culture. A single host plant or a consortium of host plants can be used to propagate AMF spores. However, the difference in the comparative efficiency of mono- and co-cultivated host plants used for the production of AMF spores and the maintenance of original AMF community composition has not been well elucidated. Here, we prepared trap culture with nutrient-poor soil from coastal sand dune vegetation collected during the dry season when the AMF spore density and relative abundance of Glomeromycota ITS2 sequences were significantly higher (<i>p</i> = <0.05) than in the wet season. The AMF communities in the soil were mainly composed of <i>Glomus</i> spp. Maize (<i>Zea mays</i> L.) and/or Sorghum (<i>Sorghum bicolor</i> (L.). Moench) were grown in trap cultures in the greenhouse. Our results demonstrated that co-cultivation of the host plants increased the production of AMF spores but, compared to mono-cultivation of host plants, did not better sustain the native AMF community compositions in the coastal sand dune soil. We propose that the co-cultivation of host plants in a trap culture broadens AMF-host plant compatibilities and thus sustains the symbiotic association of the natively diverse AMF. Therefore, the results of this study suggest that further research is needed to confirm whether the co-culturing of more than one host plant is as efficient a strategy as using a monoculture of a single host plant.https://www.mdpi.com/2309-608X/8/10/1061coastal sand dune vegetationtrap cultureAMF spore propagationco-cultivation of AMF host plantsAMF community composition |
| spellingShingle | Ugyen Wangmo Tenzin Nuttapol Noirungsee Phanthipha Runsaeng Prakrit Noppradit Lompong Klinnawee Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture Journal of Fungi coastal sand dune vegetation trap culture AMF spore propagation co-cultivation of AMF host plants AMF community composition |
| title | Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture |
| title_full | Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture |
| title_fullStr | Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture |
| title_full_unstemmed | Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture |
| title_short | Dry-Season Soil and Co-Cultivated Host Plants Enhanced Propagation of Arbuscular Mycorrhizal Fungal Spores from Sand Dune Vegetation in Trap Culture |
| title_sort | dry season soil and co cultivated host plants enhanced propagation of arbuscular mycorrhizal fungal spores from sand dune vegetation in trap culture |
| topic | coastal sand dune vegetation trap culture AMF spore propagation co-cultivation of AMF host plants AMF community composition |
| url | https://www.mdpi.com/2309-608X/8/10/1061 |
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