Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report
Abstract Background Orientia tsutsugamushi (O. tsutsugamushi), an obligate intracellular bacterium, is transmitted to humans through infected larval trombiculid mite bites, causing scrub typhus. Mixed genotypes of O. tsutsugamushi in canonical conserved genes were reported in 8–25% of blood samples...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-08-01
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| Series: | BMC Infectious Diseases |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12879-022-07682-y |
| _version_ | 1828758992144302080 |
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| author | Chuanning Tang Liyuan Zhang Yi Huang Wenhui Mai Liying Xue Gaoyu Wang Shu Wen Ruoyan Peng Kunliang Wu Xiuying Tian Hua Pei Jiang Du Kwok-Yung Yuen Jasper Fuk-Woo Chan Yongguo Du Feifei Yin |
| author_facet | Chuanning Tang Liyuan Zhang Yi Huang Wenhui Mai Liying Xue Gaoyu Wang Shu Wen Ruoyan Peng Kunliang Wu Xiuying Tian Hua Pei Jiang Du Kwok-Yung Yuen Jasper Fuk-Woo Chan Yongguo Du Feifei Yin |
| author_sort | Chuanning Tang |
| collection | DOAJ |
| description | Abstract Background Orientia tsutsugamushi (O. tsutsugamushi), an obligate intracellular bacterium, is transmitted to humans through infected larval trombiculid mite bites, causing scrub typhus. Mixed genotypes of O. tsutsugamushi in canonical conserved genes were reported in 8–25% of blood samples from patients. Yet, there are few clinical descriptions of these mixed O. tsutsugamushi-infected patients. Case presentation We report a patient with scrub typhus complicated with pulmonary involvement and hepatic dysfunction, who carried mixed genotypes of the conserved genes but had a single immune-dominant 56-kDa type-specific antigen (tsa56) genotype. The patient was successfully recovered by doxycycline treatment. Conclusions In this reported case, both patient’s eschar and blood samples have repeatedly shown the same results, i.e., no variants were discovered in tsa56 gene that bears multiple hypervariable regions. Whereas the selected highly conserved genes were identified with up to 32 variants in a 2700 base-pair concatenated sequence. The prevalence, disease severity and mechanism of these single-tsa56-genotype mixed infections remain to be investigated on a large scale with more cases. |
| first_indexed | 2024-12-11T00:50:21Z |
| format | Article |
| id | doaj.art-b7501ebf00c643e3bf097ad6161d3ef1 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-11T00:50:21Z |
| publishDate | 2022-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-b7501ebf00c643e3bf097ad6161d3ef12022-12-22T01:26:38ZengBMCBMC Infectious Diseases1471-23342022-08-012211510.1186/s12879-022-07682-yMixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case reportChuanning Tang0Liyuan Zhang1Yi Huang2Wenhui Mai3Liying Xue4Gaoyu Wang5Shu Wen6Ruoyan Peng7Kunliang Wu8Xiuying Tian9Hua Pei10Jiang Du11Kwok-Yung Yuen12Jasper Fuk-Woo Chan13Yongguo Du14Feifei Yin15Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityHaikou Maternal and Child Health HospitalKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityClinical Laboratory, People’s Hospital of Qiongzhong Li Miao Autonomous CountyKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityDepartment of Infectious Disease, The Second Affiliated Hospital of Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityAcademician Workstation of the Hainan Province, Hainan Medical UniversityClinical Laboratory, People’s Hospital of Qiongzhong Li Miao Autonomous CountyKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical UniversityAbstract Background Orientia tsutsugamushi (O. tsutsugamushi), an obligate intracellular bacterium, is transmitted to humans through infected larval trombiculid mite bites, causing scrub typhus. Mixed genotypes of O. tsutsugamushi in canonical conserved genes were reported in 8–25% of blood samples from patients. Yet, there are few clinical descriptions of these mixed O. tsutsugamushi-infected patients. Case presentation We report a patient with scrub typhus complicated with pulmonary involvement and hepatic dysfunction, who carried mixed genotypes of the conserved genes but had a single immune-dominant 56-kDa type-specific antigen (tsa56) genotype. The patient was successfully recovered by doxycycline treatment. Conclusions In this reported case, both patient’s eschar and blood samples have repeatedly shown the same results, i.e., no variants were discovered in tsa56 gene that bears multiple hypervariable regions. Whereas the selected highly conserved genes were identified with up to 32 variants in a 2700 base-pair concatenated sequence. The prevalence, disease severity and mechanism of these single-tsa56-genotype mixed infections remain to be investigated on a large scale with more cases.https://doi.org/10.1186/s12879-022-07682-yScrub typhusOrientia tsutsugamushiMixed-infectionstsa56Multi-locus sequence typing |
| spellingShingle | Chuanning Tang Liyuan Zhang Yi Huang Wenhui Mai Liying Xue Gaoyu Wang Shu Wen Ruoyan Peng Kunliang Wu Xiuying Tian Hua Pei Jiang Du Kwok-Yung Yuen Jasper Fuk-Woo Chan Yongguo Du Feifei Yin Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report BMC Infectious Diseases Scrub typhus Orientia tsutsugamushi Mixed-infections tsa56 Multi-locus sequence typing |
| title | Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report |
| title_full | Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report |
| title_fullStr | Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report |
| title_full_unstemmed | Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report |
| title_short | Mixed genotypes of Orientia tsutsugamushi in conserved genes and a single immune-dominant tsa56 genotype discovered from a patient with scrub typhus in Hainan Island, China: a case report |
| title_sort | mixed genotypes of orientia tsutsugamushi in conserved genes and a single immune dominant tsa56 genotype discovered from a patient with scrub typhus in hainan island china a case report |
| topic | Scrub typhus Orientia tsutsugamushi Mixed-infections tsa56 Multi-locus sequence typing |
| url | https://doi.org/10.1186/s12879-022-07682-y |
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