Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化)
研究了聚乙二醇二丙烯酸酯(PEGDA)作为预聚物，三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂，紫外光引发交联聚合，实现对脂肪酶固定化的方法.结果表明所制备的固定化酶与游离酶相比催化活性提高了8倍以上，且其穗定性及对环境的适应性也大大提高，在95%的乙醇中浸泡5 d后，其催化活力仍有96%之多....
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2004-09-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/zjup/1008-9497.2004.31.5.557-559 |
| _version_ | 1827303570924371968 |
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| author | WANGYan-fang(王艳芳) WANGJie(王杰) SONGXi-jin(宋锡瑾) |
| author_facet | WANGYan-fang(王艳芳) WANGJie(王杰) SONGXi-jin(宋锡瑾) |
| author_sort | WANGYan-fang(王艳芳) |
| collection | DOAJ |
| description | 研究了聚乙二醇二丙烯酸酯(PEGDA)作为预聚物，三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂，紫外光引发交联聚合，实现对脂肪酶固定化的方法.结果表明所制备的固定化酶与游离酶相比催化活性提高了8倍以上，且其穗定性及对环境的适应性也大大提高，在95%的乙醇中浸泡5 d后，其催化活力仍有96%之多. |
| first_indexed | 2024-04-24T17:03:12Z |
| format | Article |
| id | doaj.art-b75096a070ba4c1f9f651357bae364b4 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T17:03:12Z |
| publishDate | 2004-09-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-b75096a070ba4c1f9f651357bae364b42024-03-29T01:58:21ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972004-09-01315557559zjup/1008-9497.2004.31.5.557-559Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化)WANGYan-fang(王艳芳)0WANGJie(王杰)1SONGXi-jin(宋锡瑾)2 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系，浙江 杭州 310028) 1.Department of Chemistry, Zhejiang University, Hangzhou 310027, China( 1.浙江大学化学系，浙江 杭州 310028) 2.Institute of Pharmaceutical Engineering, Zhejiang University, Hangzhou 310027, China( 2.浙江大学制药工程研究所，浙江 杭州 310027)研究了聚乙二醇二丙烯酸酯(PEGDA)作为预聚物，三羟甲基丙烷三丙烯酸酯(TMPTA)为交联剂，紫外光引发交联聚合，实现对脂肪酶固定化的方法.结果表明所制备的固定化酶与游离酶相比催化活性提高了8倍以上，且其穗定性及对环境的适应性也大大提高，在95%的乙醇中浸泡5 d后，其催化活力仍有96%之多.https://doi.org/zjup/1008-9497.2004.31.5.557-559固定化脂肪酶载体 |
| spellingShingle | WANGYan-fang(王艳芳) WANGJie(王杰) SONGXi-jin(宋锡瑾) Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) Zhejiang Daxue xuebao. Lixue ban 固定化 脂肪酶 载体 |
| title | Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) |
| title_full | Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) |
| title_fullStr | Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) |
| title_full_unstemmed | Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) |
| title_short | Immobilization of lipase using the polymer of polyglycol diacrylate as carrier(脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化) |
| title_sort | immobilization of lipase using the polymer of polyglycol diacrylate as carrier 脂肪酶在聚乙二醇二丙烯酸酯交联聚合物上的固定化 |
| topic | 固定化 脂肪酶 载体 |
| url | https://doi.org/zjup/1008-9497.2004.31.5.557-559 |
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