Contribution of platelet-derived microRNAs to serum microRNAs in healthy men
Platelets are a major source of microRNAs (miRNAs) in blood. Relationships between circulating platelet-derived miRNAs were investigated to elucidate their significance as biomarkers. Total miRNAs in serum were analyzed using the 3D-Gene miRNA Oligo chip. Among 22 miRNAs that are included in platele...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-10-01
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| Series: | Platelets |
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| Online Access: | http://dx.doi.org/10.1080/09537104.2020.1810223 |
| _version_ | 1797684162096791552 |
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| author | Ichiro Wakabayashi Yoko Sotoda Ryoji Eguchi |
| author_facet | Ichiro Wakabayashi Yoko Sotoda Ryoji Eguchi |
| author_sort | Ichiro Wakabayashi |
| collection | DOAJ |
| description | Platelets are a major source of microRNAs (miRNAs) in blood. Relationships between circulating platelet-derived miRNAs were investigated to elucidate their significance as biomarkers. Total miRNAs in serum were analyzed using the 3D-Gene miRNA Oligo chip. Among 22 miRNAs that are included in platelets and play functional roles, sufficient miRNA levels for comparison were detected for 11 miRNAs (let-7b-5p, miR-16–5p, miR-17–5p, miR-24–3p, miR-107, miR-126–3p, miR-150–3p, miR-191–5p, miR-197–3p, miR-223–3p, and miR-326). Among 55 pairs prepared by these miRNAs, relatively strong correlations (Spearman’s correlation coefficient >0.8) were shown between miRNAs of 7 pairs including let-7b-5p and miR-16-5p, let-7b-5p and miR-17-5p, let-7b-5p and miR-107, miR-16–5p and miR-17-5p, miR-16-5p and miR-107, miR-17-5p and miR-107, and miR-107 and miR-126-3p. In principal component analysis, the first principal component consisted of let-7b-5p, miR-16-5p, miR-17-5p, miR-107, miR-126–3p, and miR-191-5p. These six miRNAs may be useful biomarkers that reflect platelet condition and function. |
| first_indexed | 2024-03-12T00:26:32Z |
| format | Article |
| id | doaj.art-b751743c066e4c2fb6fcc5a8497ed334 |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:26:32Z |
| publishDate | 2021-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-b751743c066e4c2fb6fcc5a8497ed3342023-09-15T10:38:08ZengTaylor & Francis GroupPlatelets0953-71041369-16352021-10-0132798498710.1080/09537104.2020.18102231810223Contribution of platelet-derived microRNAs to serum microRNAs in healthy menIchiro Wakabayashi0Yoko Sotoda1Ryoji Eguchi2Hyogo College of MedicineYamagata Saisei HospitalHyogo College of MedicinePlatelets are a major source of microRNAs (miRNAs) in blood. Relationships between circulating platelet-derived miRNAs were investigated to elucidate their significance as biomarkers. Total miRNAs in serum were analyzed using the 3D-Gene miRNA Oligo chip. Among 22 miRNAs that are included in platelets and play functional roles, sufficient miRNA levels for comparison were detected for 11 miRNAs (let-7b-5p, miR-16–5p, miR-17–5p, miR-24–3p, miR-107, miR-126–3p, miR-150–3p, miR-191–5p, miR-197–3p, miR-223–3p, and miR-326). Among 55 pairs prepared by these miRNAs, relatively strong correlations (Spearman’s correlation coefficient >0.8) were shown between miRNAs of 7 pairs including let-7b-5p and miR-16-5p, let-7b-5p and miR-17-5p, let-7b-5p and miR-107, miR-16–5p and miR-17-5p, miR-16-5p and miR-107, miR-17-5p and miR-107, and miR-107 and miR-126-3p. In principal component analysis, the first principal component consisted of let-7b-5p, miR-16-5p, miR-17-5p, miR-107, miR-126–3p, and miR-191-5p. These six miRNAs may be useful biomarkers that reflect platelet condition and function.http://dx.doi.org/10.1080/09537104.2020.1810223correlationmicrornaplateletsprincipal component |
| spellingShingle | Ichiro Wakabayashi Yoko Sotoda Ryoji Eguchi Contribution of platelet-derived microRNAs to serum microRNAs in healthy men Platelets correlation microrna platelets principal component |
| title | Contribution of platelet-derived microRNAs to serum microRNAs in healthy men |
| title_full | Contribution of platelet-derived microRNAs to serum microRNAs in healthy men |
| title_fullStr | Contribution of platelet-derived microRNAs to serum microRNAs in healthy men |
| title_full_unstemmed | Contribution of platelet-derived microRNAs to serum microRNAs in healthy men |
| title_short | Contribution of platelet-derived microRNAs to serum microRNAs in healthy men |
| title_sort | contribution of platelet derived micrornas to serum micrornas in healthy men |
| topic | correlation microrna platelets principal component |
| url | http://dx.doi.org/10.1080/09537104.2020.1810223 |
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