The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa
Introduction: Pseudomonas aeruginosa is a Gram-negative bacterium that considered as important opportunistic human pathogen. One of the mechanisms that help bacteria to tolerate survival in adverse conditions and resistance to antibiotics is biofilm formation through quorum sensing (QS) signals and...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2014-06-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/431 |
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| author | Saeed Hemati Farid Azizi-Jalilian Iraj Pakzad Morovat Taherikalani Abbas Maleki Sajedeh Karimi Azam Monjezei Zahra Mahdavi Mohamad Reza Fadavi Kourosh Sayehmiri Nourkhoda Sadeghifard |
| author_facet | Saeed Hemati Farid Azizi-Jalilian Iraj Pakzad Morovat Taherikalani Abbas Maleki Sajedeh Karimi Azam Monjezei Zahra Mahdavi Mohamad Reza Fadavi Kourosh Sayehmiri Nourkhoda Sadeghifard |
| author_sort | Saeed Hemati |
| collection | DOAJ |
| description | Introduction: Pseudomonas aeruginosa is a Gram-negative bacterium that considered as important opportunistic human pathogen. One of the mechanisms that help bacteria to tolerate survival in adverse conditions and resistance to antibiotics is biofilm formation through quorum sensing (QS) signals and toxin-antitoxin (TA) systems. QS and TA are two systems that have important roles in biofilm formation. QS is a global regulatory mechanism that enable bacteria to communicate with each other by production of auto inducers (AI) molecules in population. Because of importance biofilm formation in P. aeruginosa infections, here, we studied frequency of QS and TA genes among clinical isolates of P. aeruginosa with ability of biofilm formation.
Materials and Methods: One hundred and forty clinical isolates of P. aeruginosa were collected from Tehran and Ilam hospitals. The isolates were identified by biochemical tests. Biofilm formation was evaluated by microplate method. After DNA extraction by boiling method, the frequency of QS genes (lasIR, rhlIR), and TA genes (mazEF, relBE, hipBA, ccdAB and mqsR) were analyzed by PCR.
Results: Our results showed that maximum resistance is related to aztreonam (72.85%) antibiotic. Most of isolates were able to produce biofilm (87.15%) and the majority of them formed strong biofilm (56.42%). PCR results showed that frequency of mazEF, relBE, hipBA, ccdAB, mqsR, lasIR and rhlIR genes were 85.71, 100, 1.42, 100, 57.14, 93.57 and 83.57 percent, respectively.
Conclusion: Clinical isolates of P. aeruginosa had high ability to form biofilm, and QS and TA system genes among these isolates were very high (except hipBA genes). There are significaut correlation between biofilm for mation and present of QS and TA system genes. |
| first_indexed | 2024-12-13T04:46:20Z |
| format | Article |
| id | doaj.art-b7596562d7ed452d9c4265597c5aa4e7 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-13T04:46:20Z |
| publishDate | 2014-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-b7596562d7ed452d9c4265597c5aa4e72022-12-21T23:59:09ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472014-06-0163The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosaSaeed Hemati0Farid Azizi-Jalilian1Iraj Pakzad2Morovat Taherikalani3Abbas Maleki4Sajedeh Karimi5Azam Monjezei6Zahra Mahdavi7Mohamad Reza Fadavi8Kourosh Sayehmiri9Nourkhoda Sadeghifard10Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Prevention of Psychosocial Injuries, Research Centre, Ilam, IR Iran.Clinical Microbiology Research Center, Department of Microbiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, IR Iran.Introduction: Pseudomonas aeruginosa is a Gram-negative bacterium that considered as important opportunistic human pathogen. One of the mechanisms that help bacteria to tolerate survival in adverse conditions and resistance to antibiotics is biofilm formation through quorum sensing (QS) signals and toxin-antitoxin (TA) systems. QS and TA are two systems that have important roles in biofilm formation. QS is a global regulatory mechanism that enable bacteria to communicate with each other by production of auto inducers (AI) molecules in population. Because of importance biofilm formation in P. aeruginosa infections, here, we studied frequency of QS and TA genes among clinical isolates of P. aeruginosa with ability of biofilm formation. Materials and Methods: One hundred and forty clinical isolates of P. aeruginosa were collected from Tehran and Ilam hospitals. The isolates were identified by biochemical tests. Biofilm formation was evaluated by microplate method. After DNA extraction by boiling method, the frequency of QS genes (lasIR, rhlIR), and TA genes (mazEF, relBE, hipBA, ccdAB and mqsR) were analyzed by PCR. Results: Our results showed that maximum resistance is related to aztreonam (72.85%) antibiotic. Most of isolates were able to produce biofilm (87.15%) and the majority of them formed strong biofilm (56.42%). PCR results showed that frequency of mazEF, relBE, hipBA, ccdAB, mqsR, lasIR and rhlIR genes were 85.71, 100, 1.42, 100, 57.14, 93.57 and 83.57 percent, respectively. Conclusion: Clinical isolates of P. aeruginosa had high ability to form biofilm, and QS and TA system genes among these isolates were very high (except hipBA genes). There are significaut correlation between biofilm for mation and present of QS and TA system genes.https://ijm.tums.ac.ir/index.php/ijm/article/view/431P. aeruginosaQuorum sensingToxin-antitoxin systems |
| spellingShingle | Saeed Hemati Farid Azizi-Jalilian Iraj Pakzad Morovat Taherikalani Abbas Maleki Sajedeh Karimi Azam Monjezei Zahra Mahdavi Mohamad Reza Fadavi Kourosh Sayehmiri Nourkhoda Sadeghifard The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa Iranian Journal of Microbiology P. aeruginosa Quorum sensing Toxin-antitoxin systems |
| title | The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa |
| title_full | The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa |
| title_fullStr | The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa |
| title_full_unstemmed | The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa |
| title_short | The correlation between the presence of quorum sensing, toxin-antitoxin system genes and MIC values with ability of biofilm formation in clinical isolates of Pseudomonas aeruginosa |
| title_sort | correlation between the presence of quorum sensing toxin antitoxin system genes and mic values with ability of biofilm formation in clinical isolates of pseudomonas aeruginosa |
| topic | P. aeruginosa Quorum sensing Toxin-antitoxin systems |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/431 |
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