Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway.
Current knowledge about Periostin biology has expanded from its recognized functions in embryogenesis and bone metabolism to its roles in tissue repair and remodeling and its clinical implications in cancer. Emerging evidence suggests that Periostin plays a critical role in the mechanism of wound he...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3862800?pdf=render |
| _version_ | 1819243071656689664 |
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| author | Luciana K Rosselli-Murai Luciana O Almeida Chiara Zagni Pablo Galindo-Moreno Miguel Padial-Molina Sarah L Volk Marcelo J Murai Hector F Rios Cristiane H Squarize Rogerio M Castilho |
| author_facet | Luciana K Rosselli-Murai Luciana O Almeida Chiara Zagni Pablo Galindo-Moreno Miguel Padial-Molina Sarah L Volk Marcelo J Murai Hector F Rios Cristiane H Squarize Rogerio M Castilho |
| author_sort | Luciana K Rosselli-Murai |
| collection | DOAJ |
| description | Current knowledge about Periostin biology has expanded from its recognized functions in embryogenesis and bone metabolism to its roles in tissue repair and remodeling and its clinical implications in cancer. Emerging evidence suggests that Periostin plays a critical role in the mechanism of wound healing; however, the paracrine effect of Periostin in epithelial cell biology is still poorly understood. We found that epithelial cells are capable of producing endogenous Periostin that, unlike mesenchymal cell, cannot be secreted. Epithelial cells responded to Periostin paracrine stimuli by enhancing cellular migration and proliferation and by activating the mTOR signaling pathway. Interestingly, biomechanical stimulation of epithelial cells, which simulates tension forces that occur during initial steps of tissue healing, induced Periostin production and mTOR activation. The molecular association of Periostin and mTOR signaling was further dissected by administering rapamycin, a selective pharmacological inhibitor of mTOR, and by disruption of Raptor and Rictor scaffold proteins implicated in the regulation of mTORC1 and mTORC2 complex assembly. Both strategies resulted in ablation of Periostin-induced mitogenic and migratory activity. These results indicate that Periostin-induced epithelial migration and proliferation requires mTOR signaling. Collectively, our findings identify Periostin as a mechanical stress responsive molecule that is primarily secreted by fibroblasts during wound healing and expressed endogenously in epithelial cells resulting in the control of cellular physiology through a mechanism mediated by the mTOR signaling cascade. |
| first_indexed | 2024-12-23T14:49:52Z |
| format | Article |
| id | doaj.art-b75b515dfbe14f7ba267183dcccd8934 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T14:49:52Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-b75b515dfbe14f7ba267183dcccd89342022-12-21T17:42:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8358010.1371/journal.pone.0083580Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway.Luciana K Rosselli-MuraiLuciana O AlmeidaChiara ZagniPablo Galindo-MorenoMiguel Padial-MolinaSarah L VolkMarcelo J MuraiHector F RiosCristiane H SquarizeRogerio M CastilhoCurrent knowledge about Periostin biology has expanded from its recognized functions in embryogenesis and bone metabolism to its roles in tissue repair and remodeling and its clinical implications in cancer. Emerging evidence suggests that Periostin plays a critical role in the mechanism of wound healing; however, the paracrine effect of Periostin in epithelial cell biology is still poorly understood. We found that epithelial cells are capable of producing endogenous Periostin that, unlike mesenchymal cell, cannot be secreted. Epithelial cells responded to Periostin paracrine stimuli by enhancing cellular migration and proliferation and by activating the mTOR signaling pathway. Interestingly, biomechanical stimulation of epithelial cells, which simulates tension forces that occur during initial steps of tissue healing, induced Periostin production and mTOR activation. The molecular association of Periostin and mTOR signaling was further dissected by administering rapamycin, a selective pharmacological inhibitor of mTOR, and by disruption of Raptor and Rictor scaffold proteins implicated in the regulation of mTORC1 and mTORC2 complex assembly. Both strategies resulted in ablation of Periostin-induced mitogenic and migratory activity. These results indicate that Periostin-induced epithelial migration and proliferation requires mTOR signaling. Collectively, our findings identify Periostin as a mechanical stress responsive molecule that is primarily secreted by fibroblasts during wound healing and expressed endogenously in epithelial cells resulting in the control of cellular physiology through a mechanism mediated by the mTOR signaling cascade.http://europepmc.org/articles/PMC3862800?pdf=render |
| spellingShingle | Luciana K Rosselli-Murai Luciana O Almeida Chiara Zagni Pablo Galindo-Moreno Miguel Padial-Molina Sarah L Volk Marcelo J Murai Hector F Rios Cristiane H Squarize Rogerio M Castilho Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. PLoS ONE |
| title | Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. |
| title_full | Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. |
| title_fullStr | Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. |
| title_full_unstemmed | Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. |
| title_short | Periostin responds to mechanical stress and tension by activating the MTOR signaling pathway. |
| title_sort | periostin responds to mechanical stress and tension by activating the mtor signaling pathway |
| url | http://europepmc.org/articles/PMC3862800?pdf=render |
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