Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF)
Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In so...
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| Format: | Article |
| Language: | English |
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Croatian Society of Chemical Engineers
2021-10-01
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| Series: | Chemical and Biochemical Engineering Quarterly |
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| Online Access: | http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-3-2021.pdf |
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| author | V. Campos R. M. Kaspary M. J. Rossi T. A. D. M. Medeiros |
| author_facet | V. Campos R. M. Kaspary M. J. Rossi T. A. D. M. Medeiros |
| author_sort | V. Campos |
| collection | DOAJ |
| description | Gamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L–1 h–1 and a yield of 0.4 g g–1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L–1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L–1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation. |
| first_indexed | 2024-12-16T09:24:25Z |
| format | Article |
| id | doaj.art-b75bd287d1f34346be788ebd597c91ef |
| institution | Directory Open Access Journal |
| issn | 0352-9568 1846-5153 |
| language | English |
| last_indexed | 2024-12-16T09:24:25Z |
| publishDate | 2021-10-01 |
| publisher | Croatian Society of Chemical Engineers |
| record_format | Article |
| series | Chemical and Biochemical Engineering Quarterly |
| spelling | doaj.art-b75bd287d1f34346be788ebd597c91ef2022-12-21T22:36:41ZengCroatian Society of Chemical EngineersChemical and Biochemical Engineering Quarterly0352-95681846-51532021-10-0135333334410.15255/CABEQ.2021.1921Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF)V. Campos0R. M. Kaspary1M. J. Rossi2T. A. D. M. Medeiros3 São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/Brazi São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/Brazi Federal University of Santa Catarina, Department of Microbiology, Immunology and Parasitology, Florianópolis/SC/Brazil São Paulo State University (Unesp), Institute of Science and Technology, Sorocaba/SP/BraziGamma-Polyglutamic acid (γ-PGA) was produced from fermentation processes by inoculating the bacterium Bacillus licheniformis on different substrates. In submerged fermentation (SmF), glycerol and glucose were used as the main carbon substrates while ammonium sulfate served to provide nitrogen. In solid-state fermentation (SSF), soybean was used as the main substrate. Tests carried out in an airlift bioreactor with B. licheniformis showed a maximum productivity of 0.789 g L–1 h–1 and a yield of 0.4 g g–1. Different soybean cultivars from the 2015 and 2016 crops were used in the SSF. The BRS 1001IPRO cultivar, from the 2016 crop, showed the highest production, i.e., 1.2 g L–1 of γ-PGA. This cultivar was subjected to different levels of milling (fine, intermediate and coarse). Coarsely milled grains increased γ-PGA production to 2.7 g L–1. Moreover, coarse milling made the internal nutrients of the grains available for fermentation and this particle size did not compromise aeration during fermentation.http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-3-2021.pdfγ-polyglutamic acidbiosynthesisb. licheniformissmfssf |
| spellingShingle | V. Campos R. M. Kaspary M. J. Rossi T. A. D. M. Medeiros Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) Chemical and Biochemical Engineering Quarterly γ-polyglutamic acid biosynthesis b. licheniformis smf ssf |
| title | Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) |
| title_full | Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) |
| title_fullStr | Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) |
| title_full_unstemmed | Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) |
| title_short | Biosynthesis of -Polyglutamic Acid by Bacillus licheniformis Through Submerged Fermentation (SmF) and Solid-state Fermentation (SSF) |
| title_sort | biosynthesis of polyglutamic acid by bacillus licheniformis through submerged fermentation smf and solid state fermentation ssf |
| topic | γ-polyglutamic acid biosynthesis b. licheniformis smf ssf |
| url | http://silverstripe.fkit.hr/cabeq/assets/Uploads/07-3-2021.pdf |
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