Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State
Non-coding Xist RNA plays an essential role in X chromosome inactivation (XCI) in female mammals. It coats the X chromosome in cis and mediates the recruitment of many proteins involved in gene silencing and heterochromatinization. The molecular basis of how Xist RNA initiates chromosomal silencing...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2021.751154/full |
| _version_ | 1819125044736950272 |
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| author | Ruka Matsuura Tatsuro Nakajima Saya Ichihara Takashi Sado Takashi Sado |
| author_facet | Ruka Matsuura Tatsuro Nakajima Saya Ichihara Takashi Sado Takashi Sado |
| author_sort | Ruka Matsuura |
| collection | DOAJ |
| description | Non-coding Xist RNA plays an essential role in X chromosome inactivation (XCI) in female mammals. It coats the X chromosome in cis and mediates the recruitment of many proteins involved in gene silencing and heterochromatinization. The molecular basis of how Xist RNA initiates chromosomal silencing and what proteins participate in this process has been extensively studied and elucidated. Its involvement in the establishment and maintenance of the X-inactivated state is, however, less understood. The XistIVS allele we previously reported is peculiar in that it can initiate XCI but fails to establish the inactive state that is stably maintained and, therefore, may provide an opportunity to explore how Xist RNA contributes to establish a robust heterochromatin state. Here we demonstrate that ectopic splicing taking place to produce XistIVS RNA disturbs its function to properly establish stable XCI state. This finding warrants the potential of XistIVS RNA to provide further insight into our understanding of how Xist RNA contributes to establish sustainable heterochromatin. |
| first_indexed | 2024-12-22T07:33:53Z |
| format | Article |
| id | doaj.art-b75d2ab5990543299de630b1d3fff216 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-22T07:33:53Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-b75d2ab5990543299de630b1d3fff2162022-12-21T18:33:56ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2021-10-01910.3389/fcell.2021.751154751154Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin StateRuka Matsuura0Tatsuro Nakajima1Saya Ichihara2Takashi Sado3Takashi Sado4Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, JapanMedical Institute of Bioregulation, Kyushu University, Fukuoka, JapanDepartment of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, JapanDepartment of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, JapanAgricultural Technology and Innovation Research Institute, Kindai University, Nara, JapanNon-coding Xist RNA plays an essential role in X chromosome inactivation (XCI) in female mammals. It coats the X chromosome in cis and mediates the recruitment of many proteins involved in gene silencing and heterochromatinization. The molecular basis of how Xist RNA initiates chromosomal silencing and what proteins participate in this process has been extensively studied and elucidated. Its involvement in the establishment and maintenance of the X-inactivated state is, however, less understood. The XistIVS allele we previously reported is peculiar in that it can initiate XCI but fails to establish the inactive state that is stably maintained and, therefore, may provide an opportunity to explore how Xist RNA contributes to establish a robust heterochromatin state. Here we demonstrate that ectopic splicing taking place to produce XistIVS RNA disturbs its function to properly establish stable XCI state. This finding warrants the potential of XistIVS RNA to provide further insight into our understanding of how Xist RNA contributes to establish sustainable heterochromatin.https://www.frontiersin.org/articles/10.3389/fcell.2021.751154/fullXist RNAembryonic stem cells (ESCs)X chromosome inactivationheterochromatingene silencingRNA-FISH |
| spellingShingle | Ruka Matsuura Tatsuro Nakajima Saya Ichihara Takashi Sado Takashi Sado Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State Frontiers in Cell and Developmental Biology Xist RNA embryonic stem cells (ESCs) X chromosome inactivation heterochromatin gene silencing RNA-FISH |
| title | Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State |
| title_full | Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State |
| title_fullStr | Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State |
| title_full_unstemmed | Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State |
| title_short | Ectopic Splicing Disturbs the Function of Xist RNA to Establish the Stable Heterochromatin State |
| title_sort | ectopic splicing disturbs the function of xist rna to establish the stable heterochromatin state |
| topic | Xist RNA embryonic stem cells (ESCs) X chromosome inactivation heterochromatin gene silencing RNA-FISH |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2021.751154/full |
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