Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows
The aims of this study were to estimate the genetic variation of traditional milk coagulation properties (MCPs), milk acidity, curd firmness (CF) modeled on time t (CFt; comprising: RCTeq, rennet coagulation time estimated from the equation; CFP, the asymptotic potential curd firmness; kCF, the curd...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731115000440 |
| _version_ | 1818739682124496896 |
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| author | A. Cecchinato S. Chessa C. Ribeca C. Cipolat-Gotet T. Bobbo J. Casellas G. Bittante |
| author_facet | A. Cecchinato S. Chessa C. Ribeca C. Cipolat-Gotet T. Bobbo J. Casellas G. Bittante |
| author_sort | A. Cecchinato |
| collection | DOAJ |
| description | The aims of this study were to estimate the genetic variation of traditional milk coagulation properties (MCPs), milk acidity, curd firmness (CF) modeled on time t (CFt; comprising: RCTeq, rennet coagulation time estimated from the equation; CFP, the asymptotic potential curd firmness; kCF, the curd firming instant rate constant; and kSR, the syneresis instant rate constant) and maximum CF traits (MCF; comprising CFmax, the maximum CF value; and tmax, the time of attainment). Furthermore, we investigated 96 single nucleotide polymorphisms (SNPs) from 54 candidate genes, testing their associations with the above-listed traits. Milk and blood samples were collected from 1271 cows (each sampled once) from 85 herds. Genotyping was performed using a custom Illumina VeraCode GoldenGate approach. A Bayesian linear animal model (including the effects of herd, days in milk, parity and additive polygenic effects) was used to estimate the genetic parameters of the studied traits. The same model with the addition of the SNP genotype effect was used for our association analysis. The heritability estimates of CFt and the MCF traits (RCTeq=0.258; kCF=0.230; CFmax=0.191; tmax=0.278) were similar to those obtained using traditional MCPs (0.187 to 0.267), except for the lower estimates for CFP (0.064) and kSR (0.077). A total of 13 of the 51 tested SNPs had relevant additive effects on at least one trait. We observed associations between MCPs and SNPs in the genes encoding ATP-binding cassette sub-family G member 2 (ABCG2), chemokine ligand 2 (CCL2), growth hormone 1 (GH1), prolactin (PRL) and toll-like receptor 2 (TLR2). Whereas, CFt and the MCF traits were associated with polymorphisms in the α-s1-casein (CSN1S1), β-casein (CSN2), GH1, oxidized low-density lipoprotein receptor 1 (OLR1), phospholipase C β1 (PLCB1), PRL and signal transducer and activator of transcription 5A (STAT5A) genes. |
| first_indexed | 2024-12-18T01:28:43Z |
| format | Article |
| id | doaj.art-b75f25a61ab445788d4bda556a1fa99e |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-18T01:28:43Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-b75f25a61ab445788d4bda556a1fa99e2022-12-21T21:25:39ZengElsevierAnimal1751-73112015-01-019711041112Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cowsA. Cecchinato0S. Chessa1C. Ribeca2C. Cipolat-Gotet3T. Bobbo4J. Casellas5G. Bittante6Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyIstituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche (CNR), via Einstein, 26900 Lodi, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyDepartament de Ciència Animal i dels Aliments, Grup de Recerca en Remugants, Universitat Autònoma de Barcelona, 08193 Bellaterra, SpainDepartment of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Viale dell’Università 16, 35020 Legnaro, ItalyThe aims of this study were to estimate the genetic variation of traditional milk coagulation properties (MCPs), milk acidity, curd firmness (CF) modeled on time t (CFt; comprising: RCTeq, rennet coagulation time estimated from the equation; CFP, the asymptotic potential curd firmness; kCF, the curd firming instant rate constant; and kSR, the syneresis instant rate constant) and maximum CF traits (MCF; comprising CFmax, the maximum CF value; and tmax, the time of attainment). Furthermore, we investigated 96 single nucleotide polymorphisms (SNPs) from 54 candidate genes, testing their associations with the above-listed traits. Milk and blood samples were collected from 1271 cows (each sampled once) from 85 herds. Genotyping was performed using a custom Illumina VeraCode GoldenGate approach. A Bayesian linear animal model (including the effects of herd, days in milk, parity and additive polygenic effects) was used to estimate the genetic parameters of the studied traits. The same model with the addition of the SNP genotype effect was used for our association analysis. The heritability estimates of CFt and the MCF traits (RCTeq=0.258; kCF=0.230; CFmax=0.191; tmax=0.278) were similar to those obtained using traditional MCPs (0.187 to 0.267), except for the lower estimates for CFP (0.064) and kSR (0.077). A total of 13 of the 51 tested SNPs had relevant additive effects on at least one trait. We observed associations between MCPs and SNPs in the genes encoding ATP-binding cassette sub-family G member 2 (ABCG2), chemokine ligand 2 (CCL2), growth hormone 1 (GH1), prolactin (PRL) and toll-like receptor 2 (TLR2). Whereas, CFt and the MCF traits were associated with polymorphisms in the α-s1-casein (CSN1S1), β-casein (CSN2), GH1, oxidized low-density lipoprotein receptor 1 (OLR1), phospholipase C β1 (PLCB1), PRL and signal transducer and activator of transcription 5A (STAT5A) genes.http://www.sciencedirect.com/science/article/pii/S1751731115000440milk coagulation propertiesmilk acidityheritabilitycandidate genedairy cow |
| spellingShingle | A. Cecchinato S. Chessa C. Ribeca C. Cipolat-Gotet T. Bobbo J. Casellas G. Bittante Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows Animal milk coagulation properties milk acidity heritability candidate gene dairy cow |
| title | Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows |
| title_full | Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows |
| title_fullStr | Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows |
| title_full_unstemmed | Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows |
| title_short | Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows |
| title_sort | genetic variation and effects of candidate gene polymorphisms on coagulation properties curd firmness modeling and acidity in milk from brown swiss cows |
| topic | milk coagulation properties milk acidity heritability candidate gene dairy cow |
| url | http://www.sciencedirect.com/science/article/pii/S1751731115000440 |
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