ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector
(1) Background: Many hemipteran insects transmit plant pathogens that cause devastating crop diseases, while pest management frequently relies primarily on insecticide applications. These intense insecticide applications lead to the development of insecticide resistance, as was the case for potato p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/3/257 |
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| author | Mahnaz Kiani Zhen Fu Adrianna Szczepaniec |
| author_facet | Mahnaz Kiani Zhen Fu Adrianna Szczepaniec |
| author_sort | Mahnaz Kiani |
| collection | DOAJ |
| description | (1) Background: Many hemipteran insects transmit plant pathogens that cause devastating crop diseases, while pest management frequently relies primarily on insecticide applications. These intense insecticide applications lead to the development of insecticide resistance, as was the case for potato psyllid, <i>Bactericera cockerelli</i> (Hemiptera: Triozidae), a vector of <i>Candidatus</i> Liberibacter solanacearum, which causes zebra chip disease in potato. (2) Methods: Here, we use double-digest restriction site-associated DNA (ddRAD) to genotype eight psyllid populations (one susceptible and seven resistant to neonicotinoid insecticides). (3) Results: Association tests identified over 400 loci that were strongly segregated between susceptible and resistant populations. Several loci were located within genes involved in insecticide resistance, gene regulation, fertility, and development. Moreover, we explored the genetic structure of these eight populations and discovered that routinely utilized haplotyping was not an accurate predictor of population structure. Pairwise comparisons of the fixation index (<i>F</i><sub>ST</sub>) of populations of the same haplotype were not different from pairwise <i>F</i><sub>ST</sub> of populations that belonged to different haplotypes. (4) Conclusions: Our findings suggest that neonicotinoid insecticide resistance has a genetic basis, most likely as a result of similar selection pressure. Furthermore, our results imply that using a single maternally inherited gene marker to designate genetic lineages for potato psyllids should be re-evaluated. |
| first_indexed | 2024-03-09T19:39:45Z |
| format | Article |
| id | doaj.art-b764edc562cd43c18db1c2ef759454d7 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T19:39:45Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-b764edc562cd43c18db1c2ef759454d72023-11-24T01:43:31ZengMDPI AGInsects2075-44502022-03-0113325710.3390/insects13030257ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen VectorMahnaz Kiani0Zhen Fu1Adrianna Szczepaniec2Department of Entomology, Texas A&M AgriLife Research, 6500 Amarillo Blvd. W, Amarillo, TX 79106, USADepartment of Entomology, Texas A&M University, College Station, TX 77840, USADepartment of Agricultural Biology, Colorado State University, Fort Collins, CO 80523, USA(1) Background: Many hemipteran insects transmit plant pathogens that cause devastating crop diseases, while pest management frequently relies primarily on insecticide applications. These intense insecticide applications lead to the development of insecticide resistance, as was the case for potato psyllid, <i>Bactericera cockerelli</i> (Hemiptera: Triozidae), a vector of <i>Candidatus</i> Liberibacter solanacearum, which causes zebra chip disease in potato. (2) Methods: Here, we use double-digest restriction site-associated DNA (ddRAD) to genotype eight psyllid populations (one susceptible and seven resistant to neonicotinoid insecticides). (3) Results: Association tests identified over 400 loci that were strongly segregated between susceptible and resistant populations. Several loci were located within genes involved in insecticide resistance, gene regulation, fertility, and development. Moreover, we explored the genetic structure of these eight populations and discovered that routinely utilized haplotyping was not an accurate predictor of population structure. Pairwise comparisons of the fixation index (<i>F</i><sub>ST</sub>) of populations of the same haplotype were not different from pairwise <i>F</i><sub>ST</sub> of populations that belonged to different haplotypes. (4) Conclusions: Our findings suggest that neonicotinoid insecticide resistance has a genetic basis, most likely as a result of similar selection pressure. Furthermore, our results imply that using a single maternally inherited gene marker to designate genetic lineages for potato psyllids should be re-evaluated.https://www.mdpi.com/2075-4450/13/3/257SNP discoverypotato psyllidgenetic population structureinsecticide resistance |
| spellingShingle | Mahnaz Kiani Zhen Fu Adrianna Szczepaniec ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector Insects SNP discovery potato psyllid genetic population structure insecticide resistance |
| title | ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector |
| title_full | ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector |
| title_fullStr | ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector |
| title_full_unstemmed | ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector |
| title_short | ddRAD Sequencing Identifies Pesticide Resistance-Related Loci and Reveals New Insights into Genetic Structure of <i>Bactericera cockerelli</i> as a Plant Pathogen Vector |
| title_sort | ddrad sequencing identifies pesticide resistance related loci and reveals new insights into genetic structure of i bactericera cockerelli i as a plant pathogen vector |
| topic | SNP discovery potato psyllid genetic population structure insecticide resistance |
| url | https://www.mdpi.com/2075-4450/13/3/257 |
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