Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes
We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-...
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| Format: | Article |
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MDPI AG
2014-11-01
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| Series: | Membranes |
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| Online Access: | http://www.mdpi.com/2077-0375/4/4/764 |
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| author | Takahiko Aoki Kenji Chimura Hikaru Sugiura Yasuko Mizuno |
| author_facet | Takahiko Aoki Kenji Chimura Hikaru Sugiura Yasuko Mizuno |
| author_sort | Takahiko Aoki |
| collection | DOAJ |
| description | We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure. |
| first_indexed | 2024-03-12T06:16:05Z |
| format | Article |
| id | doaj.art-b7650f771f8348ad860cfc588f5f48b4 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T06:16:05Z |
| publishDate | 2014-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-b7650f771f8348ad860cfc588f5f48b42023-09-03T02:38:53ZengMDPI AGMembranes2077-03752014-11-014476477710.3390/membranes4040764membranes4040764Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell MembranesTakahiko Aoki0Kenji Chimura1Hikaru Sugiura2Yasuko Mizuno3Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanLaboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanLaboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, JapanToray Research Centre, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, JapanWe isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure.http://www.mdpi.com/2077-0375/4/4/764fishred blood cell membraneserythrocyteglycophorinoligosaccharidesialic acidN-glycolylneuraminic acid |
| spellingShingle | Takahiko Aoki Kenji Chimura Hikaru Sugiura Yasuko Mizuno Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes Membranes fish red blood cell membranes erythrocyte glycophorin oligosaccharide sialic acid N-glycolylneuraminic acid |
| title | Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes |
| title_full | Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes |
| title_fullStr | Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes |
| title_full_unstemmed | Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes |
| title_short | Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes |
| title_sort | structure of a sialo oligosaccharide from glycophorin in carp red blood cell membranes |
| topic | fish red blood cell membranes erythrocyte glycophorin oligosaccharide sialic acid N-glycolylneuraminic acid |
| url | http://www.mdpi.com/2077-0375/4/4/764 |
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