Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China
BackgroundThis study aimed to evaluate the performance of two different principles of HPV testing in primary cervical cancer screening and ASC-US triage in rural areas.Methods3,328 and 3,913 women were enrolled in Shanxi, China in 2017 and 2018, respectively, and screened using liquid-based cytology...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040285/full |
| _version_ | 1798019032718245888 |
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| author | Zhi-Fang Li Zhi-Fang Li Xin-Hua Jia Xin-Hua Jia Xin-Yu Ren Bei-Ke Wu Wen Chen Xiang-Xian Feng Li-Bing Wang You-Lin Qiao |
| author_facet | Zhi-Fang Li Zhi-Fang Li Xin-Hua Jia Xin-Hua Jia Xin-Yu Ren Bei-Ke Wu Wen Chen Xiang-Xian Feng Li-Bing Wang You-Lin Qiao |
| author_sort | Zhi-Fang Li |
| collection | DOAJ |
| description | BackgroundThis study aimed to evaluate the performance of two different principles of HPV testing in primary cervical cancer screening and ASC-US triage in rural areas.Methods3,328 and 3,913 women were enrolled in Shanxi, China in 2017 and 2018, respectively, and screened using liquid-based cytology and different HPV tests with a 4-year follow-up. Different screening methods commonly used in clinical practice were evaluated.ResultsIn the HPV PCR test cohort, the prevalence of HPV infection was 14.90%. A total of 38 cases of CIN2+ were identified at baseline, 2 of which were in the HPV-negative cohort and the rest in the HPV-positive cohort (2 = 186.85, p < 0.001). Fifty-three cases of CIN2+ were accumulated over 4 years. The HPV infection rate in the HPV DNA chip test cohort was 21.10%. A total of 26 CIN2+ cases were identified at baseline, all in the HPV-positive population (2 = 92.96, p < 0.001). 54 CIN2+ cases were cumulative over 4 years. At 4-year follow-up, HPV-negative results were significantly more protective against cervical intraepithelial neoplasia grade 2 or worse (CIN2+) than normal cytologic results at baseline. HPV screening was more sensitive and specific than cytologic screening (using ASC-US as the threshold) and performed better on the HPV DNA microarray test. In addition, compared with HPV 16/18 testing, sensitivity increases and specificity decreases when using HPV testing for cytologic ASC-US triage, regardless of which HPV test is used.ConclusionIn the rural areas where we implemented the study, HPV tests performed well for screening than LBC and HPV DNA chip testing performed better than HPV PCR testing in the screening cohort. Optimal screening was achieved technically when used in combination with LBC for ASC-US population triage, without thinking the feasibility for resource availability. |
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| format | Article |
| id | doaj.art-b769e7c0f3d643298a654955dbb8ac00 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T16:34:07Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-b769e7c0f3d643298a654955dbb8ac002022-12-22T04:13:54ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10402851040285Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural ChinaZhi-Fang Li0Zhi-Fang Li1Xin-Hua Jia2Xin-Hua Jia3Xin-Yu Ren4Bei-Ke Wu5Wen Chen6Xiang-Xian Feng7Li-Bing Wang8You-Lin Qiao9Department of Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Preventive Medicine, Changzhi Medical College, Changzhi, ChinaDepartment of Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaState Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, National Institute of Diagnostics and Vaccine Development in Infectious Diseases, Collaborative Innovation Center of Biologic Products, School of Public Health, Xiamen University, Xiamen, Fujian, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, ChinaSchool of Public Health, Shanxi Medical University, Taiyuan, ChinaDepartment of Epidemiology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Preventive Medicine, Changzhi Medical College, Changzhi, ChinaDepartment of Pathology, Affiliated Heping Hospital of Changzhi Meidical College, Changzhi, ChinaCenter for Global Health, School of Population Medicine and Public Health Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaBackgroundThis study aimed to evaluate the performance of two different principles of HPV testing in primary cervical cancer screening and ASC-US triage in rural areas.Methods3,328 and 3,913 women were enrolled in Shanxi, China in 2017 and 2018, respectively, and screened using liquid-based cytology and different HPV tests with a 4-year follow-up. Different screening methods commonly used in clinical practice were evaluated.ResultsIn the HPV PCR test cohort, the prevalence of HPV infection was 14.90%. A total of 38 cases of CIN2+ were identified at baseline, 2 of which were in the HPV-negative cohort and the rest in the HPV-positive cohort (2 = 186.85, p < 0.001). Fifty-three cases of CIN2+ were accumulated over 4 years. The HPV infection rate in the HPV DNA chip test cohort was 21.10%. A total of 26 CIN2+ cases were identified at baseline, all in the HPV-positive population (2 = 92.96, p < 0.001). 54 CIN2+ cases were cumulative over 4 years. At 4-year follow-up, HPV-negative results were significantly more protective against cervical intraepithelial neoplasia grade 2 or worse (CIN2+) than normal cytologic results at baseline. HPV screening was more sensitive and specific than cytologic screening (using ASC-US as the threshold) and performed better on the HPV DNA microarray test. In addition, compared with HPV 16/18 testing, sensitivity increases and specificity decreases when using HPV testing for cytologic ASC-US triage, regardless of which HPV test is used.ConclusionIn the rural areas where we implemented the study, HPV tests performed well for screening than LBC and HPV DNA chip testing performed better than HPV PCR testing in the screening cohort. Optimal screening was achieved technically when used in combination with LBC for ASC-US population triage, without thinking the feasibility for resource availability.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040285/fullhuman papillomaviruscervical cancerHPV DNA chip testHPV PCR testscreening |
| spellingShingle | Zhi-Fang Li Zhi-Fang Li Xin-Hua Jia Xin-Hua Jia Xin-Yu Ren Bei-Ke Wu Wen Chen Xiang-Xian Feng Li-Bing Wang You-Lin Qiao Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China Frontiers in Microbiology human papillomavirus cervical cancer HPV DNA chip test HPV PCR test screening |
| title | Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China |
| title_full | Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China |
| title_fullStr | Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China |
| title_full_unstemmed | Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China |
| title_short | Comparison of the performance of HPV DNA chip test and HPV PCR test in cervical cancer screening in rural China |
| title_sort | comparison of the performance of hpv dna chip test and hpv pcr test in cervical cancer screening in rural china |
| topic | human papillomavirus cervical cancer HPV DNA chip test HPV PCR test screening |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040285/full |
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