Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting

Background: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated...

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Main Authors: Lars Saemann, Lena Wernstedt, Sabine Pohl, Markus Stiller, Jan Willsch, Britt Hofmann, Gábor Veres, Andreas Simm, Gábor Szabó
Format: Article
Language:English
Published: MDPI AG 2023-08-01
Series:Journal of Clinical Medicine
Subjects:
Online Access:https://www.mdpi.com/2077-0383/12/17/5454
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author Lars Saemann
Lena Wernstedt
Sabine Pohl
Markus Stiller
Jan Willsch
Britt Hofmann
Gábor Veres
Andreas Simm
Gábor Szabó
author_facet Lars Saemann
Lena Wernstedt
Sabine Pohl
Markus Stiller
Jan Willsch
Britt Hofmann
Gábor Veres
Andreas Simm
Gábor Szabó
author_sort Lars Saemann
collection DOAJ
description Background: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated the impact of age in patients treated with CABG on endothelial function in saphenous vein grafts. Methods: We mounted the saphenous vein graft segments of CABG patients < 70 (<i>n</i> = 33) and ≥70 (<i>n</i> = 40) years of age in organ bath chambers and exposed them to potassium chloride (KCl) and phenylephrine (PE) to test the receptor-independent and -dependent contractility, followed by exposure to acetylcholine (ACh) and sodium nitroprusside (SNP) to test the endothelial-dependent and -independent relaxation. Results: The maximal contraction induced by KCl (2.3 ± 1.8 vs. 1.8 ± 2 g) was stronger in patients ≥ 70 years of age. The relative contraction induced by PE in % of KCl (167 ± 64 vs. 163 ± 59%) was similar between groups. Patients aged < 70 years showed a higher endothelial-dependent relaxation induced by acetylcholine than patients ≥ 70 years (51 ± 27 vs. 42 ± 18%). The relaxation induced by SNP was similar between both groups. Conclusions: The endothelial function of saphenous vein bypass grafts decreases during aging. Thus, age should be considered when improving graft maintenance.
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spelling doaj.art-b76b5b6c1cb84c89abd2d8cdd187f1112023-11-19T08:20:53ZengMDPI AGJournal of Clinical Medicine2077-03832023-08-011217545410.3390/jcm12175454Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass GraftingLars Saemann0Lena Wernstedt1Sabine Pohl2Markus Stiller3Jan Willsch4Britt Hofmann5Gábor Veres6Andreas Simm7Gábor Szabó8Department of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyBackground: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated the impact of age in patients treated with CABG on endothelial function in saphenous vein grafts. Methods: We mounted the saphenous vein graft segments of CABG patients < 70 (<i>n</i> = 33) and ≥70 (<i>n</i> = 40) years of age in organ bath chambers and exposed them to potassium chloride (KCl) and phenylephrine (PE) to test the receptor-independent and -dependent contractility, followed by exposure to acetylcholine (ACh) and sodium nitroprusside (SNP) to test the endothelial-dependent and -independent relaxation. Results: The maximal contraction induced by KCl (2.3 ± 1.8 vs. 1.8 ± 2 g) was stronger in patients ≥ 70 years of age. The relative contraction induced by PE in % of KCl (167 ± 64 vs. 163 ± 59%) was similar between groups. Patients aged < 70 years showed a higher endothelial-dependent relaxation induced by acetylcholine than patients ≥ 70 years (51 ± 27 vs. 42 ± 18%). The relaxation induced by SNP was similar between both groups. Conclusions: The endothelial function of saphenous vein bypass grafts decreases during aging. Thus, age should be considered when improving graft maintenance.https://www.mdpi.com/2077-0383/12/17/5454coronary artery bypass grafting (CABG)saphenous veinagingsenescencecoronary artery diseaseendothelium
spellingShingle Lars Saemann
Lena Wernstedt
Sabine Pohl
Markus Stiller
Jan Willsch
Britt Hofmann
Gábor Veres
Andreas Simm
Gábor Szabó
Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
Journal of Clinical Medicine
coronary artery bypass grafting (CABG)
saphenous vein
aging
senescence
coronary artery disease
endothelium
title Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
title_full Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
title_fullStr Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
title_full_unstemmed Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
title_short Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
title_sort impact of age on endothelial function of saphenous vein grafts in coronary artery bypass grafting
topic coronary artery bypass grafting (CABG)
saphenous vein
aging
senescence
coronary artery disease
endothelium
url https://www.mdpi.com/2077-0383/12/17/5454
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