Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting
Background: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated...
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MDPI AG
2023-08-01
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author | Lars Saemann Lena Wernstedt Sabine Pohl Markus Stiller Jan Willsch Britt Hofmann Gábor Veres Andreas Simm Gábor Szabó |
author_facet | Lars Saemann Lena Wernstedt Sabine Pohl Markus Stiller Jan Willsch Britt Hofmann Gábor Veres Andreas Simm Gábor Szabó |
author_sort | Lars Saemann |
collection | DOAJ |
description | Background: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated the impact of age in patients treated with CABG on endothelial function in saphenous vein grafts. Methods: We mounted the saphenous vein graft segments of CABG patients < 70 (<i>n</i> = 33) and ≥70 (<i>n</i> = 40) years of age in organ bath chambers and exposed them to potassium chloride (KCl) and phenylephrine (PE) to test the receptor-independent and -dependent contractility, followed by exposure to acetylcholine (ACh) and sodium nitroprusside (SNP) to test the endothelial-dependent and -independent relaxation. Results: The maximal contraction induced by KCl (2.3 ± 1.8 vs. 1.8 ± 2 g) was stronger in patients ≥ 70 years of age. The relative contraction induced by PE in % of KCl (167 ± 64 vs. 163 ± 59%) was similar between groups. Patients aged < 70 years showed a higher endothelial-dependent relaxation induced by acetylcholine than patients ≥ 70 years (51 ± 27 vs. 42 ± 18%). The relaxation induced by SNP was similar between both groups. Conclusions: The endothelial function of saphenous vein bypass grafts decreases during aging. Thus, age should be considered when improving graft maintenance. |
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spelling | doaj.art-b76b5b6c1cb84c89abd2d8cdd187f1112023-11-19T08:20:53ZengMDPI AGJournal of Clinical Medicine2077-03832023-08-011217545410.3390/jcm12175454Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass GraftingLars Saemann0Lena Wernstedt1Sabine Pohl2Markus Stiller3Jan Willsch4Britt Hofmann5Gábor Veres6Andreas Simm7Gábor Szabó8Department of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyDepartment of Cardiac Surgery, University Hospital Halle, Ernst-Grube-Straße 40, 06120 Halle (Saale), GermanyBackground: An intact and functionally preserved endothelial layer in the graft is crucial for myocardial perfusion and graft patency after coronary artery bypass grafting (CABG). We hypothesized that old age is a risk factor for decreased endothelial function of bypass grafts. Thus, we investigated the impact of age in patients treated with CABG on endothelial function in saphenous vein grafts. Methods: We mounted the saphenous vein graft segments of CABG patients < 70 (<i>n</i> = 33) and ≥70 (<i>n</i> = 40) years of age in organ bath chambers and exposed them to potassium chloride (KCl) and phenylephrine (PE) to test the receptor-independent and -dependent contractility, followed by exposure to acetylcholine (ACh) and sodium nitroprusside (SNP) to test the endothelial-dependent and -independent relaxation. Results: The maximal contraction induced by KCl (2.3 ± 1.8 vs. 1.8 ± 2 g) was stronger in patients ≥ 70 years of age. The relative contraction induced by PE in % of KCl (167 ± 64 vs. 163 ± 59%) was similar between groups. Patients aged < 70 years showed a higher endothelial-dependent relaxation induced by acetylcholine than patients ≥ 70 years (51 ± 27 vs. 42 ± 18%). The relaxation induced by SNP was similar between both groups. Conclusions: The endothelial function of saphenous vein bypass grafts decreases during aging. Thus, age should be considered when improving graft maintenance.https://www.mdpi.com/2077-0383/12/17/5454coronary artery bypass grafting (CABG)saphenous veinagingsenescencecoronary artery diseaseendothelium |
spellingShingle | Lars Saemann Lena Wernstedt Sabine Pohl Markus Stiller Jan Willsch Britt Hofmann Gábor Veres Andreas Simm Gábor Szabó Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting Journal of Clinical Medicine coronary artery bypass grafting (CABG) saphenous vein aging senescence coronary artery disease endothelium |
title | Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting |
title_full | Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting |
title_fullStr | Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting |
title_full_unstemmed | Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting |
title_short | Impact of Age on Endothelial Function of Saphenous Vein Grafts in Coronary Artery Bypass Grafting |
title_sort | impact of age on endothelial function of saphenous vein grafts in coronary artery bypass grafting |
topic | coronary artery bypass grafting (CABG) saphenous vein aging senescence coronary artery disease endothelium |
url | https://www.mdpi.com/2077-0383/12/17/5454 |
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