Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital
ABSTRACT Background: Mupirocin has been used for the treatment of skin infections and for the eradication of the nasal carriage of Methicillin -resistant Staphylococcus aureus (MRSA). The increased use of this antibiotic has been accompanied by its resistance, resulting in treatment failures....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2013-02-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/2736/12-%204694_u.pdf |
| _version_ | 1819173354139025408 |
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| author | Jayakumar S Meerabai M Shameem Banu A.S Renu Mathew Kalyani M Binesh Lal Y |
| author_facet | Jayakumar S Meerabai M Shameem Banu A.S Renu Mathew Kalyani M Binesh Lal Y |
| author_sort | Jayakumar S |
| collection | DOAJ |
| description | ABSTRACT
Background: Mupirocin has been used for the treatment of
skin infections and for the eradication of the nasal carriage
of Methicillin -resistant Staphylococcus aureus (MRSA). The
increased use of this antibiotic has been accompanied by its
resistance, resulting in treatment failures.
Objective: This study was aimed at determining the prevalences
of low and high level Mupirocin resistance among the clinical
isolates of Staphylococcus species which were obtained from
pyogenic infections.
Material and Method: Clinical samples such as wound swabs,
tissues and pus which were submitted to the microbiology
laboratory during a period of six months were screened for the
growth of Staphylococcus species, which were identified as
Staphylococcus aureus and Coagulase negative Staphylococcus
species by the routine microbiological procedures. All the
isolates were tested for their Mupirocin susceptibilities by using
5 and 200 µg discs and their resistance was confirmed from
their Minimum Inhibitory Concentrations (MICs).
Result: Out of 400 samples, 150 samples grew Staphylococcus
species, of which 113 were Staphylococcus aureus and 37 were
Coagulase negative Staphylococcus (CoNS). Only 5(3.3%)
mupirocin resistant Staphylococcus species: three high level and
two low level strains were detected. The MICs for the two low
level and three high level Mupirocin resistant strains were 256
mg/L and ≥512mg/L each respectively.
Conclusion: We conclude that the screening for mupirocin
resistance, in terms of high-level and low-level resistance
among the Staphylococcus species from patients with skin and
soft tissue infections is warranted and that it is important for
the clinicians in selecting the appropriate, empirical, topical,
antimicrobial therapy. It also provides useful information about
the prevalence of these resistant pathogens. |
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| format | Article |
| id | doaj.art-b76c0a18f0044c43867ef625245723e2 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-22T20:21:44Z |
| publishDate | 2013-02-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-b76c0a18f0044c43867ef625245723e22022-12-21T18:13:50ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2013-02-017223824210.7860/JCDR/2013/4694.2736Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care HospitalJayakumar S0Meerabai M1Shameem Banu A.S2Renu Mathew3Kalyani M4 Binesh Lal Y5Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.Department of Microbiology, Saveetha Medical College & Hospital, Saveetha University, Thandalam, Kancheepuram District-602 105, Tamilnadu, India.ABSTRACT Background: Mupirocin has been used for the treatment of skin infections and for the eradication of the nasal carriage of Methicillin -resistant Staphylococcus aureus (MRSA). The increased use of this antibiotic has been accompanied by its resistance, resulting in treatment failures. Objective: This study was aimed at determining the prevalences of low and high level Mupirocin resistance among the clinical isolates of Staphylococcus species which were obtained from pyogenic infections. Material and Method: Clinical samples such as wound swabs, tissues and pus which were submitted to the microbiology laboratory during a period of six months were screened for the growth of Staphylococcus species, which were identified as Staphylococcus aureus and Coagulase negative Staphylococcus species by the routine microbiological procedures. All the isolates were tested for their Mupirocin susceptibilities by using 5 and 200 µg discs and their resistance was confirmed from their Minimum Inhibitory Concentrations (MICs). Result: Out of 400 samples, 150 samples grew Staphylococcus species, of which 113 were Staphylococcus aureus and 37 were Coagulase negative Staphylococcus (CoNS). Only 5(3.3%) mupirocin resistant Staphylococcus species: three high level and two low level strains were detected. The MICs for the two low level and three high level Mupirocin resistant strains were 256 mg/L and ≥512mg/L each respectively. Conclusion: We conclude that the screening for mupirocin resistance, in terms of high-level and low-level resistance among the Staphylococcus species from patients with skin and soft tissue infections is warranted and that it is important for the clinicians in selecting the appropriate, empirical, topical, antimicrobial therapy. It also provides useful information about the prevalence of these resistant pathogens.https://jcdr.net/articles/PDF/2736/12-%204694_u.pdfmethicillin-resistant staphylococcus aureus (mrsa)coagulase negative staphylococcus (cons)high and low level mupirocin resistance |
| spellingShingle | Jayakumar S Meerabai M Shameem Banu A.S Renu Mathew Kalyani M Binesh Lal Y Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital Journal of Clinical and Diagnostic Research methicillin-resistant staphylococcus aureus (mrsa) coagulase negative staphylococcus (cons) high and low level mupirocin resistance |
| title | Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital |
| title_full | Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital |
| title_fullStr | Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital |
| title_full_unstemmed | Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital |
| title_short | Prevalence of High and Low Level Mupirocin Resistance among Staphylococcal Isolates from Skin Infection in a Tertiary Care Hospital |
| title_sort | prevalence of high and low level mupirocin resistance among staphylococcal isolates from skin infection in a tertiary care hospital |
| topic | methicillin-resistant staphylococcus aureus (mrsa) coagulase negative staphylococcus (cons) high and low level mupirocin resistance |
| url | https://jcdr.net/articles/PDF/2736/12-%204694_u.pdf |
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