Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach
The current study aimed to evaluate the naturally occurring antimicrobial and antidiabetic potential of various <i>Echium humile</i> (<i>E. humile</i>) solvent extracts (hexane, dichloromethane, ethyl acetate, methanol and aqueous). The bioactive compounds were identified usi...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/11/9/1131 |
| _version_ | 1797503330284470272 |
|---|---|
| author | Kaïss Aouadi Hafedh Hajlaoui Soumaya Arraouadi Siwar Ghannay Mejdi Snoussi Adel Kadri |
| author_facet | Kaïss Aouadi Hafedh Hajlaoui Soumaya Arraouadi Siwar Ghannay Mejdi Snoussi Adel Kadri |
| author_sort | Kaïss Aouadi |
| collection | DOAJ |
| description | The current study aimed to evaluate the naturally occurring antimicrobial and antidiabetic potential of various <i>Echium humile</i> (<i>E. humile</i>) solvent extracts (hexane, dichloromethane, ethyl acetate, methanol and aqueous). The bioactive compounds were identified using HPLC–MS, revealing the presence of sixteen phytochemical compounds, with the most abundant being <i>p</i>-coumaric acid, followed by 4,5-di-<i>O</i>-caffeoylquinic acid, trans-ferulic acid and acacetin. Furthermore, <i>E. humile</i> extracts showed marked antimicrobial properties against human pathogen strains, with MIC values for the most relevant extracts (methanol and ethyl acetate) ranging from 0.19 to 6.25 mg/mL and 0.39 to 12.50 mg/mL, respectively. Likewise, methanol was found to be bactericidal towards <i>S. aureus</i>, <i>B. cereus</i> and <i>M. luteus</i>, fungicidal against <i>P. catenulatum</i> and <i>F. oxysporum</i> and have a bacteriostatic/fungicidal effect for the other strains. In addition, the <i>E. humile</i> methanolic extract had the greatest α-glucosidase inhibitory effect (IC<sub>50</sub> = 0.06 ± 0.29 mg/mL), which is higher than the standard drug, acarbose (IC<sub>50</sub> = 0.80 ± 1.81 mg/mL) and the aqueous extract (IC<sub>50</sub> = 0.70 ± 0.67 mg/mL). A correlation study between the major phytochemicals and the evaluated activities was investigated. Docking studies evidenced that most of the identified phenolic compounds showed strong interactions into the binding sites of <i>S. aureus</i> tyrosyl-tRNA synthetase and human lysosomal acid-α-glucosidase, confirming their suitable inhibitory effect. In summary, these results may provide rational support to explore the clinical efficacy of <i>E. humile</i> and its secondary metabolites in the treatment of dual diabetes and infections. |
| first_indexed | 2024-03-10T03:49:03Z |
| format | Article |
| id | doaj.art-b76e45897eee4f288ee0b6155113955c |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T03:49:03Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-b76e45897eee4f288ee0b6155113955c2023-11-23T09:01:54ZengMDPI AGPlants2223-77472022-04-01119113110.3390/plants11091131Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico ApproachKaïss Aouadi0Hafedh Hajlaoui1Soumaya Arraouadi2Siwar Ghannay3Mejdi Snoussi4Adel Kadri5Department of Chemistry, College of Science, Qassim University, Buraidah 51452, Saudi ArabiaResearch Unit Valorization and Optimization of Resource Exploitation (UR16ES04), Faculty of Science and Technology of Sidi Bouzid, Campus University Agricultural City, University of Kairouan, Sidi Bouzid 9100, TunisiaRegional Center of Agricultural Research (CRRA) Sidi Bouzid, Gafsa Road Km 6, PB 357, Sidi Bouzid 9100, TunisiaDepartment of Chemistry, College of Science, Qassim University, Buraidah 51452, Saudi ArabiaDepartment of Biology, College of Science, Hail University, Ha’il 2440, Saudi ArabiaDepartment of Chemistry, Faculty of Science of Sfax, University of Sfax, B.P. 1171, Sfax 3000, TunisiaThe current study aimed to evaluate the naturally occurring antimicrobial and antidiabetic potential of various <i>Echium humile</i> (<i>E. humile</i>) solvent extracts (hexane, dichloromethane, ethyl acetate, methanol and aqueous). The bioactive compounds were identified using HPLC–MS, revealing the presence of sixteen phytochemical compounds, with the most abundant being <i>p</i>-coumaric acid, followed by 4,5-di-<i>O</i>-caffeoylquinic acid, trans-ferulic acid and acacetin. Furthermore, <i>E. humile</i> extracts showed marked antimicrobial properties against human pathogen strains, with MIC values for the most relevant extracts (methanol and ethyl acetate) ranging from 0.19 to 6.25 mg/mL and 0.39 to 12.50 mg/mL, respectively. Likewise, methanol was found to be bactericidal towards <i>S. aureus</i>, <i>B. cereus</i> and <i>M. luteus</i>, fungicidal against <i>P. catenulatum</i> and <i>F. oxysporum</i> and have a bacteriostatic/fungicidal effect for the other strains. In addition, the <i>E. humile</i> methanolic extract had the greatest α-glucosidase inhibitory effect (IC<sub>50</sub> = 0.06 ± 0.29 mg/mL), which is higher than the standard drug, acarbose (IC<sub>50</sub> = 0.80 ± 1.81 mg/mL) and the aqueous extract (IC<sub>50</sub> = 0.70 ± 0.67 mg/mL). A correlation study between the major phytochemicals and the evaluated activities was investigated. Docking studies evidenced that most of the identified phenolic compounds showed strong interactions into the binding sites of <i>S. aureus</i> tyrosyl-tRNA synthetase and human lysosomal acid-α-glucosidase, confirming their suitable inhibitory effect. In summary, these results may provide rational support to explore the clinical efficacy of <i>E. humile</i> and its secondary metabolites in the treatment of dual diabetes and infections.https://www.mdpi.com/2223-7747/11/9/1131antidiabeticantimicrobial<i>Echium humile</i>extractsHPLC–MS analysismolecular docking |
| spellingShingle | Kaïss Aouadi Hafedh Hajlaoui Soumaya Arraouadi Siwar Ghannay Mejdi Snoussi Adel Kadri Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach Plants antidiabetic antimicrobial <i>Echium humile</i> extracts HPLC–MS analysis molecular docking |
| title | Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach |
| title_full | Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach |
| title_fullStr | Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach |
| title_full_unstemmed | Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach |
| title_short | Phytochemical Profiling, Antimicrobial and α-Glucosidase Inhibitory Potential of Phenolic-Enriched Extracts of the Aerial Parts from <i>Echium humile</i> Desf.: In Vitro Combined with In Silico Approach |
| title_sort | phytochemical profiling antimicrobial and α glucosidase inhibitory potential of phenolic enriched extracts of the aerial parts from i echium humile i desf in vitro combined with in silico approach |
| topic | antidiabetic antimicrobial <i>Echium humile</i> extracts HPLC–MS analysis molecular docking |
| url | https://www.mdpi.com/2223-7747/11/9/1131 |
| work_keys_str_mv | AT kaissaouadi phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach AT hafedhhajlaoui phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach AT soumayaarraouadi phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach AT siwarghannay phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach AT mejdisnoussi phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach AT adelkadri phytochemicalprofilingantimicrobialandaglucosidaseinhibitorypotentialofphenolicenrichedextractsoftheaerialpartsfromiechiumhumileidesfinvitrocombinedwithinsilicoapproach |