Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria
This study was carried out to isolate and identify the keratinophilic fungi and other dermatophytes present in soils of Otolo Nnewi, Nnewi north local government area, Anambra state, Nigeria. Eighty soil samples were collected from four habitats (playgrounds, abattoir, public parks, and poultry fa...
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| Format: | Article |
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The National Information and Documentation Centre (NIDOC) , Egypt
2019-06-01
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| Series: | Novel Research in Microbiology Journal |
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| Online Access: | https://nrmj.journals.ekb.eg/article_37211_cb6d00545c5e09257d1f60de900b3ce0.pdf |
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| author | Eze, E.M. Ezebialu, C.U. Unegbu, V.N. Nneji, I.R. |
| author_facet | Eze, E.M. Ezebialu, C.U. Unegbu, V.N. Nneji, I.R. |
| author_sort | Eze, E.M. |
| collection | DOAJ |
| description | This study was carried out to isolate and identify the keratinophilic fungi and other dermatophytes present in
soils of Otolo Nnewi, Nnewi north local government area, Anambra state, Nigeria. Eighty soil samples were
collected from four habitats (playgrounds, abattoir, public parks, and poultry farms) of Otolo Nnewi; and were
screened for the presence of keratinophilic fungi and dermatophytes, using hair baiting techniques for isolation of
these fungi. Of which, 69 soil samples (86%) were positive for fungal growth. Fungal growth appearing on the
baits after 2-4 weeks of incubation at 37°C were macroscopically and microscopically examined, and were also
cultured on Sabroaud dextrose agar (SDA). These fungal isolates were identified on the basis of colony and
microscopic features. A total of 150 isolates of keratinophiic fungi were detected. The isolated fungi were
classified into 19 species belonging to 11 genera mainly; Chrysosporium indicum, C. tropicum, Aspergillus
flavus, Microsporum gypseum and Trichophyton terrestre were isolated frequently. C. indicum (13%) was the
most predominant isolated species; C. tropicum (12%) was the second, followed by A. flavus (11%). In the current
study; M. gypseum (9.3%) was the most common isolated dermatophyte, followed by T. terrestre (6%) and T.
rubrum (6%). M. canis was isolated only from soils of abattoir and public parks. Moreover, C. zonatum, T.
mentagrophytes, Alternaria alternata and Fusarium oxysporum were also isolated. There was significant
statistical difference (p<0.05) between the keratinophilic fungi and dermatophytes isolated from soil samples of
these different habitats. Results obtained from this study indicated the wide occurrence of keratinophilic fungi in
the studied area, which were potential agents of human and animals fungal diseases. |
| first_indexed | 2024-04-24T08:30:27Z |
| format | Article |
| id | doaj.art-b774c1c14dbb4bd394e6a2a0c67abbad |
| institution | Directory Open Access Journal |
| issn | 2537-0286 2537-0294 |
| language | English |
| last_indexed | 2024-04-24T08:30:27Z |
| publishDate | 2019-06-01 |
| publisher | The National Information and Documentation Centre (NIDOC) , Egypt |
| record_format | Article |
| series | Novel Research in Microbiology Journal |
| spelling | doaj.art-b774c1c14dbb4bd394e6a2a0c67abbad2024-04-16T20:24:39ZengThe National Information and Documentation Centre (NIDOC) , EgyptNovel Research in Microbiology Journal2537-02862537-02942019-06-013337938610.21608/nrmj.2019.37211Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, NigeriaEze, E.M.0Ezebialu, C.U.1Unegbu, V.N.2Nneji, I.R.3Department of Microbiology, Novena University, Ogume, Delta state, NigeriaDepartment of Microbiology, Godfrey Okoye University, Thinkers Corner Ugwumu-nike, NigeriaDepartment of Microbiology, Renaissance University, Ugboawka, Enugu State, NigeriaDepartment of Microbiology, Legacy University , Okija, Anambra State, NigeriaThis study was carried out to isolate and identify the keratinophilic fungi and other dermatophytes present in soils of Otolo Nnewi, Nnewi north local government area, Anambra state, Nigeria. Eighty soil samples were collected from four habitats (playgrounds, abattoir, public parks, and poultry farms) of Otolo Nnewi; and were screened for the presence of keratinophilic fungi and dermatophytes, using hair baiting techniques for isolation of these fungi. Of which, 69 soil samples (86%) were positive for fungal growth. Fungal growth appearing on the baits after 2-4 weeks of incubation at 37°C were macroscopically and microscopically examined, and were also cultured on Sabroaud dextrose agar (SDA). These fungal isolates were identified on the basis of colony and microscopic features. A total of 150 isolates of keratinophiic fungi were detected. The isolated fungi were classified into 19 species belonging to 11 genera mainly; Chrysosporium indicum, C. tropicum, Aspergillus flavus, Microsporum gypseum and Trichophyton terrestre were isolated frequently. C. indicum (13%) was the most predominant isolated species; C. tropicum (12%) was the second, followed by A. flavus (11%). In the current study; M. gypseum (9.3%) was the most common isolated dermatophyte, followed by T. terrestre (6%) and T. rubrum (6%). M. canis was isolated only from soils of abattoir and public parks. Moreover, C. zonatum, T. mentagrophytes, Alternaria alternata and Fusarium oxysporum were also isolated. There was significant statistical difference (p<0.05) between the keratinophilic fungi and dermatophytes isolated from soil samples of these different habitats. Results obtained from this study indicated the wide occurrence of keratinophilic fungi in the studied area, which were potential agents of human and animals fungal diseases.https://nrmj.journals.ekb.eg/article_37211_cb6d00545c5e09257d1f60de900b3ce0.pdfsoilhair baitingkeratinophilic fungidermatophytesanambra statenigeria |
| spellingShingle | Eze, E.M. Ezebialu, C.U. Unegbu, V.N. Nneji, I.R. Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria Novel Research in Microbiology Journal soil hair baiting keratinophilic fungi dermatophytes anambra state nigeria |
| title | Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria |
| title_full | Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria |
| title_fullStr | Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria |
| title_full_unstemmed | Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria |
| title_short | Prevalence of keratinophiic fungi and other dermatophytes from soils of Nnewi in Anambra state, Nigeria |
| title_sort | prevalence of keratinophiic fungi and other dermatophytes from soils of nnewi in anambra state nigeria |
| topic | soil hair baiting keratinophilic fungi dermatophytes anambra state nigeria |
| url | https://nrmj.journals.ekb.eg/article_37211_cb6d00545c5e09257d1f60de900b3ce0.pdf |
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