Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables
Fenvalerate residues in fruits and vegetables may result in biological immune system disorders. Current sensor detection methods are harsh due to the shortcomings of antibody preparation and preservation conditions. Therefore, developing a recognition material with strong specificity, good stability...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.957745/full |
| _version_ | 1818022055291912192 |
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| author | Le Zhang Yiliu Zheng Hua Shao Ming Xiao Jianchun Sun Maojun Jin Fen Jin Jing Wang A. M. Abd El-Aty A. M. Abd El-Aty A. M. Abd El-Aty Yongxin She |
| author_facet | Le Zhang Yiliu Zheng Hua Shao Ming Xiao Jianchun Sun Maojun Jin Fen Jin Jing Wang A. M. Abd El-Aty A. M. Abd El-Aty A. M. Abd El-Aty Yongxin She |
| author_sort | Le Zhang |
| collection | DOAJ |
| description | Fenvalerate residues in fruits and vegetables may result in biological immune system disorders. Current sensor detection methods are harsh due to the shortcomings of antibody preparation and preservation conditions. Therefore, developing a recognition material with strong specificity, good stability, and low cost is of practical significance in designing a sensitive, simple, and rapid method. This study used precipitation polymerization to synthesize molecularly imprinted polymers (MIPs). The MIP was prepared into a fiber membrane using the electrostatic spinning method. After that, the fenvalerate hapten-mouse IgG-Eu fluorescent probe was synthesized, and the side flow chromatography strip was constructed to determine fenvalerate in vegetables using the immunocompetition method. The results showed that the adsorption capacity of MIP to fenvalerate was 3.65, and the adsorption capacity on MIPFM (an electrospinning membrane containing the fenvalerate MIPs) was five times that of free MIP. The test strip showed good linearity with R2 = 0.9761 within the range of 50 μg/L-1,000 μg/L. In conclusion, substituting fenvalerate monoclonal antibodies with a molecularly imprinted electrospinning membrane is ideal for rapid onsite detection of pyrethroids. |
| first_indexed | 2024-04-14T08:27:19Z |
| format | Article |
| id | doaj.art-b775fc2a45174c4aa086b4ecd4d8d0a3 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-14T08:27:19Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-b775fc2a45174c4aa086b4ecd4d8d0a32022-12-22T02:04:01ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.957745957745Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetablesLe Zhang0Yiliu Zheng1Hua Shao2Ming Xiao3Jianchun Sun4Maojun Jin5Fen Jin6Jing Wang7A. M. Abd El-Aty8A. M. Abd El-Aty9A. M. Abd El-Aty10Yongxin She11Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaAcademy of Agriculture and Forestry Sciences, Qinghai University, Xining, ChinaInspection and Testing Center of Agricultural Products of Tibetan Autonomous Region, Lhasa, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan, ChinaDepartment of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, EgyptDepartment of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, TurkeyInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, ChinaFenvalerate residues in fruits and vegetables may result in biological immune system disorders. Current sensor detection methods are harsh due to the shortcomings of antibody preparation and preservation conditions. Therefore, developing a recognition material with strong specificity, good stability, and low cost is of practical significance in designing a sensitive, simple, and rapid method. This study used precipitation polymerization to synthesize molecularly imprinted polymers (MIPs). The MIP was prepared into a fiber membrane using the electrostatic spinning method. After that, the fenvalerate hapten-mouse IgG-Eu fluorescent probe was synthesized, and the side flow chromatography strip was constructed to determine fenvalerate in vegetables using the immunocompetition method. The results showed that the adsorption capacity of MIP to fenvalerate was 3.65, and the adsorption capacity on MIPFM (an electrospinning membrane containing the fenvalerate MIPs) was five times that of free MIP. The test strip showed good linearity with R2 = 0.9761 within the range of 50 μg/L-1,000 μg/L. In conclusion, substituting fenvalerate monoclonal antibodies with a molecularly imprinted electrospinning membrane is ideal for rapid onsite detection of pyrethroids.https://www.frontiersin.org/articles/10.3389/fnut.2022.957745/fullfenvaleratemolecularly imprinted polymerelectrospinning membranetest stripfluorescence |
| spellingShingle | Le Zhang Yiliu Zheng Hua Shao Ming Xiao Jianchun Sun Maojun Jin Fen Jin Jing Wang A. M. Abd El-Aty A. M. Abd El-Aty A. M. Abd El-Aty Yongxin She Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables Frontiers in Nutrition fenvalerate molecularly imprinted polymer electrospinning membrane test strip fluorescence |
| title | Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| title_full | Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| title_fullStr | Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| title_full_unstemmed | Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| title_short | Development of a time-resolved fluorescence microsphere Eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| title_sort | development of a time resolved fluorescence microsphere eu lateral flow test strip based on a molecularly imprinted electrospun nanofiber membrane for determination of fenvalerate in vegetables |
| topic | fenvalerate molecularly imprinted polymer electrospinning membrane test strip fluorescence |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.957745/full |
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