Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis
To identify pathogen-induced genes distinct from those involved in systemic acquired resistance, we used cDNA-amplified fragment length polymorphism to examine RNA levels in Arabidopsis thaliana wild type, nim1-1, and salicylate hydroxylase-expressing plants after inoculation with an incompatible is...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-10-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1235 |
| _version_ | 1818915027165380608 |
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| author | Gregory J. Rairdan Nicole M. Donofrio Terrence P. Delaney |
| author_facet | Gregory J. Rairdan Nicole M. Donofrio Terrence P. Delaney |
| author_sort | Gregory J. Rairdan |
| collection | DOAJ |
| description | To identify pathogen-induced genes distinct from those involved in systemic acquired resistance, we used cDNA-amplified fragment length polymorphism to examine RNA levels in Arabidopsis thaliana wild type, nim1-1, and salicylate hydroxylase-expressing plants after inoculation with an incompatible isolate of the downy mildew pathogen Peronospora parasitica. Fifteen genes are described, which define three response profiles on the basis of whether their induction requires salicylic acid (SA) accumulation and NIM1/NPR1 activity, SA alone, or neither. Sequence analysis shows that the genes include a calcium binding protein related to TCH3, a protein containing ankyrin repeats and potential transmembrane domains, three glutathione S-transferase gene family members, and a number of small, putatively secreted proteins. We further characterized this set of genes by assessing their expression patterns in each of the three plant lines after inoculation with a compatible P. parasitica isolate and after treatment with the SA analog 2,6-dichloroisonicotinic acid. Some of the genes within subclasses showed different requirements for SA accumulation and NIM1/NPR1 activity, depending upon which elicitor was used, indicating that those genes were not coordinately regulated and that the regulatory pathways are more complex than simple linear models would indicate. |
| first_indexed | 2024-12-19T23:55:45Z |
| format | Article |
| id | doaj.art-b776b9bbf5bb4e729d86c3cdbeb1cfff |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-19T23:55:45Z |
| publishDate | 2001-10-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-b776b9bbf5bb4e729d86c3cdbeb1cfff2022-12-21T20:01:01ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-10-0114101235124610.1094/MPMI.2001.14.10.1235Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in ArabidopsisGregory J. RairdanNicole M. DonofrioTerrence P. DelaneyTo identify pathogen-induced genes distinct from those involved in systemic acquired resistance, we used cDNA-amplified fragment length polymorphism to examine RNA levels in Arabidopsis thaliana wild type, nim1-1, and salicylate hydroxylase-expressing plants after inoculation with an incompatible isolate of the downy mildew pathogen Peronospora parasitica. Fifteen genes are described, which define three response profiles on the basis of whether their induction requires salicylic acid (SA) accumulation and NIM1/NPR1 activity, SA alone, or neither. Sequence analysis shows that the genes include a calcium binding protein related to TCH3, a protein containing ankyrin repeats and potential transmembrane domains, three glutathione S-transferase gene family members, and a number of small, putatively secreted proteins. We further characterized this set of genes by assessing their expression patterns in each of the three plant lines after inoculation with a compatible P. parasitica isolate and after treatment with the SA analog 2,6-dichloroisonicotinic acid. Some of the genes within subclasses showed different requirements for SA accumulation and NIM1/NPR1 activity, depending upon which elicitor was used, indicating that those genes were not coordinately regulated and that the regulatory pathways are more complex than simple linear models would indicate.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1235calmodulinCXc750ECS1gene expression profilingNahG plantsORFX |
| spellingShingle | Gregory J. Rairdan Nicole M. Donofrio Terrence P. Delaney Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis Molecular Plant-Microbe Interactions calmodulin CXc750 ECS1 gene expression profiling NahG plants ORFX |
| title | Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis |
| title_full | Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis |
| title_fullStr | Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis |
| title_full_unstemmed | Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis |
| title_short | Salicylic Acid and NIM1/NPR1-Independent Gene Induction by Incompatible Peronospora parasitica in Arabidopsis |
| title_sort | salicylic acid and nim1 npr1 independent gene induction by incompatible peronospora parasitica in arabidopsis |
| topic | calmodulin CXc750 ECS1 gene expression profiling NahG plants ORFX |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.10.1235 |
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