| Summary: | <i>Bacillus</i> species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, <i>Bacillus glycinifermentans</i> MGMM1, isolated from the rhizosphere of a weed plant (<i>Senna occidentalis</i>) and assayed its phenotypic characteristics, as well as antifungal and biocontrol ability. The whole genome analysis of MGMM1 identified 4259 putative coding sequences, with an encoding density of 95.75% attributed to biological functions, including genes involved in stimulating plant growth, such as acetolactate synthase, <i>als</i>S, and genes involved in the resistance to heavy metal antimony (<i>ars</i>B and <i>ars</i>C). AntiSMASH revealed the presence of biosynthetic gene clusters plipastatin, fengycin, laterocidine, geobacillin II, lichenysin, butirosin A and schizokinen. Tests in vitro confirmed that MGMM1 exhibited antifungal activity against <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407, <i>Alternaria alternata</i>, <i>F. graminearum</i> and <i>F.</i> spp. and produce protease, lipase amylase and cellulase. <i>Bacillus glycinifermentans</i> MGMM1 demonstrated proteolytic (4.82 ± 1.04 U/mL), amylolytic (0.84 ± 0.05 U/mL) and cellulosic (0.35 ± 0.02 U/mL) enzymatic activities, as well as indole-3-acetic acid production (48.96 ± 1.43 μg/mL). Moreover, the probiotic strain MGMM1 demonstrated a high biocontrol potential of inhibiting (up to 51.45 ± 8.08%) the development of tomato disease caused by <i>Forl</i> ZUM2407. These results suggest that <i>B. glycinifermentans</i> MGMM1 has significant potential as a biocontrol, plant growth-promoting agent in agriculture.
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