Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications
<i>Bacillus</i> species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, <i>Bacill...
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MDPI AG
2023-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/6/1410 |
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| author | Daniel Mawuena Afordoanyi Roderic Gilles Claret Diabankana Ernest Nailevich Komissarov Evgenii Sergeyevich Kuchaev Shamil Zavdatovich Validov |
| author_facet | Daniel Mawuena Afordoanyi Roderic Gilles Claret Diabankana Ernest Nailevich Komissarov Evgenii Sergeyevich Kuchaev Shamil Zavdatovich Validov |
| author_sort | Daniel Mawuena Afordoanyi |
| collection | DOAJ |
| description | <i>Bacillus</i> species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, <i>Bacillus glycinifermentans</i> MGMM1, isolated from the rhizosphere of a weed plant (<i>Senna occidentalis</i>) and assayed its phenotypic characteristics, as well as antifungal and biocontrol ability. The whole genome analysis of MGMM1 identified 4259 putative coding sequences, with an encoding density of 95.75% attributed to biological functions, including genes involved in stimulating plant growth, such as acetolactate synthase, <i>als</i>S, and genes involved in the resistance to heavy metal antimony (<i>ars</i>B and <i>ars</i>C). AntiSMASH revealed the presence of biosynthetic gene clusters plipastatin, fengycin, laterocidine, geobacillin II, lichenysin, butirosin A and schizokinen. Tests in vitro confirmed that MGMM1 exhibited antifungal activity against <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407, <i>Alternaria alternata</i>, <i>F. graminearum</i> and <i>F.</i> spp. and produce protease, lipase amylase and cellulase. <i>Bacillus glycinifermentans</i> MGMM1 demonstrated proteolytic (4.82 ± 1.04 U/mL), amylolytic (0.84 ± 0.05 U/mL) and cellulosic (0.35 ± 0.02 U/mL) enzymatic activities, as well as indole-3-acetic acid production (48.96 ± 1.43 μg/mL). Moreover, the probiotic strain MGMM1 demonstrated a high biocontrol potential of inhibiting (up to 51.45 ± 8.08%) the development of tomato disease caused by <i>Forl</i> ZUM2407. These results suggest that <i>B. glycinifermentans</i> MGMM1 has significant potential as a biocontrol, plant growth-promoting agent in agriculture. |
| first_indexed | 2024-03-11T02:08:34Z |
| format | Article |
| id | doaj.art-b779b8956ff84cee947704faadce9119 |
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| language | English |
| last_indexed | 2024-03-11T02:08:34Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-b779b8956ff84cee947704faadce91192023-11-18T11:41:31ZengMDPI AGMicroorganisms2076-26072023-05-01116141010.3390/microorganisms11061410Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological ApplicationsDaniel Mawuena Afordoanyi0Roderic Gilles Claret Diabankana1Ernest Nailevich Komissarov2Evgenii Sergeyevich Kuchaev3Shamil Zavdatovich Validov4Laboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, RussiaLaboratory of Molecular Genetics and Microbiology Methods, Kazan Scientific Center of Russian Academy of Sciences, 420111 Kazan, Russia<i>Bacillus</i> species have gained much attention based on their phenotypic characteristics and their genetic architecture as biological control agents and plant growth-promotor with bioremediation potential. In this study, we analyzed the whole genome of a novel strain, <i>Bacillus glycinifermentans</i> MGMM1, isolated from the rhizosphere of a weed plant (<i>Senna occidentalis</i>) and assayed its phenotypic characteristics, as well as antifungal and biocontrol ability. The whole genome analysis of MGMM1 identified 4259 putative coding sequences, with an encoding density of 95.75% attributed to biological functions, including genes involved in stimulating plant growth, such as acetolactate synthase, <i>als</i>S, and genes involved in the resistance to heavy metal antimony (<i>ars</i>B and <i>ars</i>C). AntiSMASH revealed the presence of biosynthetic gene clusters plipastatin, fengycin, laterocidine, geobacillin II, lichenysin, butirosin A and schizokinen. Tests in vitro confirmed that MGMM1 exhibited antifungal activity against <i>Fusarium oxysporum</i> f.sp. <i>radicis-lycopersici</i> (<i>Forl</i>) ZUM2407, <i>Alternaria alternata</i>, <i>F. graminearum</i> and <i>F.</i> spp. and produce protease, lipase amylase and cellulase. <i>Bacillus glycinifermentans</i> MGMM1 demonstrated proteolytic (4.82 ± 1.04 U/mL), amylolytic (0.84 ± 0.05 U/mL) and cellulosic (0.35 ± 0.02 U/mL) enzymatic activities, as well as indole-3-acetic acid production (48.96 ± 1.43 μg/mL). Moreover, the probiotic strain MGMM1 demonstrated a high biocontrol potential of inhibiting (up to 51.45 ± 8.08%) the development of tomato disease caused by <i>Forl</i> ZUM2407. These results suggest that <i>B. glycinifermentans</i> MGMM1 has significant potential as a biocontrol, plant growth-promoting agent in agriculture.https://www.mdpi.com/2076-2607/11/6/1410<i>Bacillus glycinifermentans</i>phenotypic properties<i>Fusarium oxysporum</i>biocontrolfull genome |
| spellingShingle | Daniel Mawuena Afordoanyi Roderic Gilles Claret Diabankana Ernest Nailevich Komissarov Evgenii Sergeyevich Kuchaev Shamil Zavdatovich Validov Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications Microorganisms <i>Bacillus glycinifermentans</i> phenotypic properties <i>Fusarium oxysporum</i> biocontrol full genome |
| title | Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications |
| title_full | Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications |
| title_fullStr | Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications |
| title_full_unstemmed | Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications |
| title_short | Characterization of a Novel <i>Bacillus glycinifermentans</i> Strain MGMM1 Based on Full Genome Analysis and Phenotypic Properties for Biotechnological Applications |
| title_sort | characterization of a novel i bacillus glycinifermentans i strain mgmm1 based on full genome analysis and phenotypic properties for biotechnological applications |
| topic | <i>Bacillus glycinifermentans</i> phenotypic properties <i>Fusarium oxysporum</i> biocontrol full genome |
| url | https://www.mdpi.com/2076-2607/11/6/1410 |
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