Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)

Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among vari...

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Main Authors: Soosaimanickam Maria Packiam, Cyril Emmanuel, Kathirvelu Baskar, Savarimuthu Ignacimuthu
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2015-08-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=en
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author Soosaimanickam Maria Packiam
Cyril Emmanuel
Kathirvelu Baskar
Savarimuthu Ignacimuthu
author_facet Soosaimanickam Maria Packiam
Cyril Emmanuel
Kathirvelu Baskar
Savarimuthu Ignacimuthu
author_sort Soosaimanickam Maria Packiam
collection DOAJ
description Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among various phytopesticidal formulations, neem and pongam oils at 1:1 ratio, called PONNEEM showed significant feeding deterrent activity against H. armigera at 20 ppm concentration and wasgenotoxic to H. armigera (P>0.001). The comet parameters, namely tail moment (arbitrary units), tail length (µm) and tail DNA (%) were observed at all the concentrations of PONNEEM. Statistically significant changes in all the comet parameters of H. armigera were observed at 20 ppm (P<0.001). Feeding deterrent and genotoxicity effect of PONNEEM could be applied as phytopesticide for controlling the lepidopteran insect pests.
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spelling doaj.art-b77b6b342b354f868f61831d5a3e5dee2022-12-21T23:20:48ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-08-0158448749310.1590/S1516-8913201500141S1516-89132015000400487Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)Soosaimanickam Maria PackiamCyril EmmanuelKathirvelu BaskarSavarimuthu IgnacimuthuNewly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among various phytopesticidal formulations, neem and pongam oils at 1:1 ratio, called PONNEEM showed significant feeding deterrent activity against H. armigera at 20 ppm concentration and wasgenotoxic to H. armigera (P>0.001). The comet parameters, namely tail moment (arbitrary units), tail length (µm) and tail DNA (%) were observed at all the concentrations of PONNEEM. Statistically significant changes in all the comet parameters of H. armigera were observed at 20 ppm (P<0.001). Feeding deterrent and genotoxicity effect of PONNEEM could be applied as phytopesticide for controlling the lepidopteran insect pests.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=enGenotoxicityPONNEEMFeeding deterrentHelicoverpa armigera
spellingShingle Soosaimanickam Maria Packiam
Cyril Emmanuel
Kathirvelu Baskar
Savarimuthu Ignacimuthu
Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
Brazilian Archives of Biology and Technology
Genotoxicity
PONNEEM
Feeding deterrent
Helicoverpa armigera
title Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
title_full Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
title_fullStr Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
title_full_unstemmed Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
title_short Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
title_sort feeding deterrent and genotoxicity analysis of a novel phytopesticide by using comet assay against helicoverpa armigera hubner lepidoptera noctuidae
topic Genotoxicity
PONNEEM
Feeding deterrent
Helicoverpa armigera
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=en
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