Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)
Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among vari...
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Format: | Article |
Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2015-08-01
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Series: | Brazilian Archives of Biology and Technology |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=en |
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author | Soosaimanickam Maria Packiam Cyril Emmanuel Kathirvelu Baskar Savarimuthu Ignacimuthu |
author_facet | Soosaimanickam Maria Packiam Cyril Emmanuel Kathirvelu Baskar Savarimuthu Ignacimuthu |
author_sort | Soosaimanickam Maria Packiam |
collection | DOAJ |
description | Newly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among various phytopesticidal formulations, neem and pongam oils at 1:1 ratio, called PONNEEM showed significant feeding deterrent activity against H. armigera at 20 ppm concentration and wasgenotoxic to H. armigera (P>0.001). The comet parameters, namely tail moment (arbitrary units), tail length (µm) and tail DNA (%) were observed at all the concentrations of PONNEEM. Statistically significant changes in all the comet parameters of H. armigera were observed at 20 ppm (P<0.001). Feeding deterrent and genotoxicity effect of PONNEEM could be applied as phytopesticide for controlling the lepidopteran insect pests. |
first_indexed | 2024-12-14T02:09:29Z |
format | Article |
id | doaj.art-b77b6b342b354f868f61831d5a3e5dee |
institution | Directory Open Access Journal |
issn | 1678-4324 |
language | English |
last_indexed | 2024-12-14T02:09:29Z |
publishDate | 2015-08-01 |
publisher | Instituto de Tecnologia do Paraná (Tecpar) |
record_format | Article |
series | Brazilian Archives of Biology and Technology |
spelling | doaj.art-b77b6b342b354f868f61831d5a3e5dee2022-12-21T23:20:48ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242015-08-0158448749310.1590/S1516-8913201500141S1516-89132015000400487Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae)Soosaimanickam Maria PackiamCyril EmmanuelKathirvelu BaskarSavarimuthu IgnacimuthuNewly developed Phytopesticidal formulations from pongam and neem oils were evaluated for their feeding deterrent activity using leaf disc choice and no-choice methods, and genotoxic study using comet assay against Helicoverpa armigera at different concentrations of 5, 10, 15, and 20 ppm. Among various phytopesticidal formulations, neem and pongam oils at 1:1 ratio, called PONNEEM showed significant feeding deterrent activity against H. armigera at 20 ppm concentration and wasgenotoxic to H. armigera (P>0.001). The comet parameters, namely tail moment (arbitrary units), tail length (µm) and tail DNA (%) were observed at all the concentrations of PONNEEM. Statistically significant changes in all the comet parameters of H. armigera were observed at 20 ppm (P<0.001). Feeding deterrent and genotoxicity effect of PONNEEM could be applied as phytopesticide for controlling the lepidopteran insect pests.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=enGenotoxicityPONNEEMFeeding deterrentHelicoverpa armigera |
spellingShingle | Soosaimanickam Maria Packiam Cyril Emmanuel Kathirvelu Baskar Savarimuthu Ignacimuthu Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) Brazilian Archives of Biology and Technology Genotoxicity PONNEEM Feeding deterrent Helicoverpa armigera |
title | Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) |
title_full | Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) |
title_fullStr | Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) |
title_full_unstemmed | Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) |
title_short | Feeding Deterrent and Genotoxicity Analysis of a novel Phytopesticide by using Comet Assay against Helicoverpa armigera (HÜbner) (Lepidoptera: Noctuidae) |
title_sort | feeding deterrent and genotoxicity analysis of a novel phytopesticide by using comet assay against helicoverpa armigera hubner lepidoptera noctuidae |
topic | Genotoxicity PONNEEM Feeding deterrent Helicoverpa armigera |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400487&lng=en&tlng=en |
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