Replication of cyclobutane pyrimidine dimer analogue by Ex Taq DNA polymerase
We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine (CVU)-containing ODN at the 5'-terminal. This method forms d(T-CVU) as a cyclobutane pyrimidine dimer (CPD) analogue between the 3'-terminal thymidine and the 5'...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2007-01-01
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| Series: | Science and Technology of Advanced Materials |
| Online Access: | http://www.iop.org/EJ/abstract/1468-6996/8/4/A14 |
| Summary: | We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine (CVU)-containing ODN at the 5'-terminal. This method forms d(T-CVU) as a cyclobutane pyrimidine dimer (CPD) analogue between the 3'-terminal thymidine and the 5'-terminal CVU of two oligodeoxynucleotides (ODNs). In this study, we performed PCR using a DNA template containing d(T-CVU). Then, we found that two adenines were incorporated opposite the d(T-CVU). |
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| ISSN: | 1468-6996 1878-5514 |