Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts
<i>Aloe vera</i>, <i>Mentha arvensis</i> (mint), <i>Coriandrum sativum</i> (coriander), and <i>Cymbopogon citratus</i> (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/12/779 |
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| author | Bilal Ahmad Li Chang Usama Qamar Satti Sami ur Rehman Huma Arshad Ghazala Mustafa Uzma Shaukat Fenghua Wang Chunyi Tong |
| author_facet | Bilal Ahmad Li Chang Usama Qamar Satti Sami ur Rehman Huma Arshad Ghazala Mustafa Uzma Shaukat Fenghua Wang Chunyi Tong |
| author_sort | Bilal Ahmad |
| collection | DOAJ |
| description | <i>Aloe vera</i>, <i>Mentha arvensis</i> (mint), <i>Coriandrum sativum</i> (coriander), and <i>Cymbopogon citratus</i> (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. The zones of inhibition (ZOI) were shown by <i>Escherichia coli</i> (<i>E. coli</i>) (1.9, 2.1, 1.7, and 2 mm), followed by <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (1.8, 1.7, 1.6, and 1.9 mm), against coriander, mint, <i>Aloe vera</i>, and lemongrass, respectively. MIC and MBC values of <i>E. coli,</i> and <i>S. aureus</i> ranged from 7 to 8 µg/mL. Overall, this study demonstrates that Ag-NPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agent for the treatment of bacterial infection. |
| first_indexed | 2024-03-09T17:19:27Z |
| format | Article |
| id | doaj.art-b7836891d16d4f909b0cc8e42616c013 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:19:27Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-b7836891d16d4f909b0cc8e42616c0132023-11-24T13:20:53ZengMDPI AGBioengineering2306-53542022-12-0191277910.3390/bioengineering9120779Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant ExtractsBilal Ahmad0Li Chang1Usama Qamar Satti2Sami ur Rehman3Huma Arshad4Ghazala Mustafa5Uzma Shaukat6Fenghua Wang7Chunyi Tong8College of Biology, Hunan University, Changsha 410082, ChinaCollege of Biology, Hunan University, Changsha 410082, ChinaKey State Laboratory of Mechanical Behaviour of Materials, Department of Materials Science and Engineering, Xi’an Jiaotong University, Xi’an 710049, ChinaDepartment of Materials Science and Engineering, Institute of Space Technology, Islamabad 44000, PakistanDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 44000, PakistanDepartment of Plant Sciences, Quaid-i-Azam University, Islamabad 44000, PakistanNational Institute of Health (NIH) Park Road, Chak Shahzad, Islamabad 45550, PakistanInstitute of Physical Education, Xinjiang Normal University, 102 Xinyi Rd., Urumqi 830054, ChinaCollege of Biology, Hunan University, Changsha 410082, China<i>Aloe vera</i>, <i>Mentha arvensis</i> (mint), <i>Coriandrum sativum</i> (coriander), and <i>Cymbopogon citratus</i> (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. The zones of inhibition (ZOI) were shown by <i>Escherichia coli</i> (<i>E. coli</i>) (1.9, 2.1, 1.7, and 2 mm), followed by <i>Staphylococcus aureus</i> (<i>S. aureus</i>) (1.8, 1.7, 1.6, and 1.9 mm), against coriander, mint, <i>Aloe vera</i>, and lemongrass, respectively. MIC and MBC values of <i>E. coli,</i> and <i>S. aureus</i> ranged from 7 to 8 µg/mL. Overall, this study demonstrates that Ag-NPs exhibit a strong antimicrobial activity and thus might be developed as a new type of antimicrobial agent for the treatment of bacterial infection.https://www.mdpi.com/2306-5354/9/12/779Ag-NPsgreen synthesis<i>Aloe vera</i>anti-bacterialphytotoxicity |
| spellingShingle | Bilal Ahmad Li Chang Usama Qamar Satti Sami ur Rehman Huma Arshad Ghazala Mustafa Uzma Shaukat Fenghua Wang Chunyi Tong Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts Bioengineering Ag-NPs green synthesis <i>Aloe vera</i> anti-bacterial phytotoxicity |
| title | Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts |
| title_full | Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts |
| title_fullStr | Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts |
| title_full_unstemmed | Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts |
| title_short | Phyto-Synthesis, Characterization, and In Vitro Antibacterial Activity of Silver Nanoparticles Using Various Plant Extracts |
| title_sort | phyto synthesis characterization and in vitro antibacterial activity of silver nanoparticles using various plant extracts |
| topic | Ag-NPs green synthesis <i>Aloe vera</i> anti-bacterial phytotoxicity |
| url | https://www.mdpi.com/2306-5354/9/12/779 |
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