Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device
Abstract Nano-scale extracellular vesicles are lipid-bilayer delimited particles that are naturally secreted by all cells and have emerged as valuable biomarkers for a wide range of diseases. Efficient isolation of small extracellular vesicles while maintaining yield and purity is crucial to harvest...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45409-4 |
| _version_ | 1797559927893393408 |
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| author | Manju Sharma Maulee Sheth Holly M. Poling Damaris Kuhnell Scott M. Langevin Leyla Esfandiari |
| author_facet | Manju Sharma Maulee Sheth Holly M. Poling Damaris Kuhnell Scott M. Langevin Leyla Esfandiari |
| author_sort | Manju Sharma |
| collection | DOAJ |
| description | Abstract Nano-scale extracellular vesicles are lipid-bilayer delimited particles that are naturally secreted by all cells and have emerged as valuable biomarkers for a wide range of diseases. Efficient isolation of small extracellular vesicles while maintaining yield and purity is crucial to harvest their potential in diagnostic, prognostic, and therapeutic applications. Most conventional methods of isolation suffer from significant shortcomings, including low purity or yield, long duration, need for large sample volumes, specialized equipment, trained personnel, and high costs. To address some of these challenges, our group has reported a novel insulator-based dielectrophoretic device for rapid isolation of small extracellular vesicles from biofluids and cell culture media based on their size and dielectric properties. In this study, we report a comprehensive characterization of small extracellular vesicles isolated from cancer-patients’ biofluids at a twofold enrichment using the device. The three-fold characterization that was performed using conventional flow cytometry, advanced imaging flow cytometry, and microRNA sequencing indicated high yield and purity of the isolated small extracellular vesicles. The device thus offers an efficient platform for rapid isolation while maintaining biomolecular integrity. |
| first_indexed | 2024-03-10T17:53:12Z |
| format | Article |
| id | doaj.art-b783d98f56b34dadad099e28bdb3ec8d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:53:12Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b783d98f56b34dadad099e28bdb3ec8d2023-11-20T09:18:30ZengNature PortfolioScientific Reports2045-23222023-10-0113111210.1038/s41598-023-45409-4Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip deviceManju Sharma0Maulee Sheth1Holly M. Poling2Damaris Kuhnell3Scott M. Langevin4Leyla Esfandiari5Department of Biomedical Engineering, College of Engineering and Applied Sciences, University of CincinnatiDepartment of Biomedical Engineering, College of Engineering and Applied Sciences, University of CincinnatiDepartment of Biomedical Engineering, College of Engineering and Applied Sciences, University of CincinnatiDepartment of Environmental and Health Sciences, College of Medicine, University of CincinnatiLarner College of Medicine, University of VermontDepartment of Biomedical Engineering, College of Engineering and Applied Sciences, University of CincinnatiAbstract Nano-scale extracellular vesicles are lipid-bilayer delimited particles that are naturally secreted by all cells and have emerged as valuable biomarkers for a wide range of diseases. Efficient isolation of small extracellular vesicles while maintaining yield and purity is crucial to harvest their potential in diagnostic, prognostic, and therapeutic applications. Most conventional methods of isolation suffer from significant shortcomings, including low purity or yield, long duration, need for large sample volumes, specialized equipment, trained personnel, and high costs. To address some of these challenges, our group has reported a novel insulator-based dielectrophoretic device for rapid isolation of small extracellular vesicles from biofluids and cell culture media based on their size and dielectric properties. In this study, we report a comprehensive characterization of small extracellular vesicles isolated from cancer-patients’ biofluids at a twofold enrichment using the device. The three-fold characterization that was performed using conventional flow cytometry, advanced imaging flow cytometry, and microRNA sequencing indicated high yield and purity of the isolated small extracellular vesicles. The device thus offers an efficient platform for rapid isolation while maintaining biomolecular integrity.https://doi.org/10.1038/s41598-023-45409-4 |
| spellingShingle | Manju Sharma Maulee Sheth Holly M. Poling Damaris Kuhnell Scott M. Langevin Leyla Esfandiari Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device Scientific Reports |
| title | Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device |
| title_full | Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device |
| title_fullStr | Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device |
| title_full_unstemmed | Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device |
| title_short | Rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label-free lab-on-a-chip device |
| title_sort | rapid purification and multiparametric characterization of circulating small extracellular vesicles utilizing a label free lab on a chip device |
| url | https://doi.org/10.1038/s41598-023-45409-4 |
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