Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death
<p>Abstract</p> <p>Background</p> <p>Neuroblastoma is a solid tumour of childhood often with an unfavourable outcome. One common genetic feature in aggressive tumours is 1p-deletion.</p> <p>The α-enolase (<it>ENO1</it>) gene is located in chromos...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2005-12-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/5/161 |
| _version_ | 1811279254037463040 |
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| author | Li Lingli Zaibak Faten Carén Helena Krona Cecilia Ejeskär Katarina Martinsson Tommy Ioannou Panayiotis A |
| author_facet | Li Lingli Zaibak Faten Carén Helena Krona Cecilia Ejeskär Katarina Martinsson Tommy Ioannou Panayiotis A |
| author_sort | Li Lingli |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Neuroblastoma is a solid tumour of childhood often with an unfavourable outcome. One common genetic feature in aggressive tumours is 1p-deletion.</p> <p>The α-enolase (<it>ENO1</it>) gene is located in chromosome region 1p36.2, within the common region of deletion in neuroblastoma. One alternative translated product of the <it>ENO1 </it>gene, known as <it>MBP-1</it>, acts as a negative regulator of the <it>c-myc </it>oncogene, making the <it>ENO1 </it>gene a candidate as a tumour suppressor gene.</p> <p>Methods</p> <p>Methods used in this study are transfection of cDNA-vectors and <it>in vitro </it>transcribed mRNA, cell growth assay, TUNEL-assay, real-time RT-PCR (TaqMan) for expression studies, genomic sequencing and DHPLC for mutation detection.</p> <p>Results</p> <p>Here we demonstrate that transfection of <it>ENO1 </it>cDNA into 1p-deleted neuroblastoma cell lines causes' reduced number of viable cells over time compared to a negative control and that it induces apoptosis. Interestingly, a similar but much stronger dose-dependent reduction of cell growth was observed by transfection of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells. These effects could also be shown in non-neuroblastoma cells (293-cells), indicating <it>ENO1 </it>to have general tumour suppressor activity.</p> <p>Expression of <it>ENO1 </it>is detectable in primary neuroblastomas of all different stages and no difference in the level of expression can be detected between 1p-deleted and 1p-intact tumour samples. Although small numbers (11 primary neuroblastomas), there is some evidence that Stage 4 tumours has a lower level of <it>ENO1</it>-mRNA than Stage 2 tumours (p = 0.01). However, mutation screening of 44 primary neuroblastomas of all different stages, failed to detect any mutations.</p> <p>Conclusion</p> <p>Our studies indicate that <it>ENO1 </it>has tumour suppressor activity and that high level of <it>ENO1 </it>expression has growth inhibitory effects.</p> |
| first_indexed | 2024-04-13T00:50:00Z |
| format | Article |
| id | doaj.art-b783dec7f4134183b590eb7da6f0565e |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-04-13T00:50:00Z |
| publishDate | 2005-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-b783dec7f4134183b590eb7da6f0565e2022-12-22T03:09:53ZengBMCBMC Cancer1471-24072005-12-015116110.1186/1471-2407-5-161Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell deathLi LingliZaibak FatenCarén HelenaKrona CeciliaEjeskär KatarinaMartinsson TommyIoannou Panayiotis A<p>Abstract</p> <p>Background</p> <p>Neuroblastoma is a solid tumour of childhood often with an unfavourable outcome. One common genetic feature in aggressive tumours is 1p-deletion.</p> <p>The α-enolase (<it>ENO1</it>) gene is located in chromosome region 1p36.2, within the common region of deletion in neuroblastoma. One alternative translated product of the <it>ENO1 </it>gene, known as <it>MBP-1</it>, acts as a negative regulator of the <it>c-myc </it>oncogene, making the <it>ENO1 </it>gene a candidate as a tumour suppressor gene.</p> <p>Methods</p> <p>Methods used in this study are transfection of cDNA-vectors and <it>in vitro </it>transcribed mRNA, cell growth assay, TUNEL-assay, real-time RT-PCR (TaqMan) for expression studies, genomic sequencing and DHPLC for mutation detection.</p> <p>Results</p> <p>Here we demonstrate that transfection of <it>ENO1 </it>cDNA into 1p-deleted neuroblastoma cell lines causes' reduced number of viable cells over time compared to a negative control and that it induces apoptosis. Interestingly, a similar but much stronger dose-dependent reduction of cell growth was observed by transfection of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells. These effects could also be shown in non-neuroblastoma cells (293-cells), indicating <it>ENO1 </it>to have general tumour suppressor activity.</p> <p>Expression of <it>ENO1 </it>is detectable in primary neuroblastomas of all different stages and no difference in the level of expression can be detected between 1p-deleted and 1p-intact tumour samples. Although small numbers (11 primary neuroblastomas), there is some evidence that Stage 4 tumours has a lower level of <it>ENO1</it>-mRNA than Stage 2 tumours (p = 0.01). However, mutation screening of 44 primary neuroblastomas of all different stages, failed to detect any mutations.</p> <p>Conclusion</p> <p>Our studies indicate that <it>ENO1 </it>has tumour suppressor activity and that high level of <it>ENO1 </it>expression has growth inhibitory effects.</p>http://www.biomedcentral.com/1471-2407/5/161 |
| spellingShingle | Li Lingli Zaibak Faten Carén Helena Krona Cecilia Ejeskär Katarina Martinsson Tommy Ioannou Panayiotis A Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death BMC Cancer |
| title | Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death |
| title_full | Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death |
| title_fullStr | Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death |
| title_full_unstemmed | Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death |
| title_short | Introduction of <it>in vitro </it>transcribed <it>ENO1 </it>mRNA into neuroblastoma cells induces cell death |
| title_sort | introduction of it in vitro it transcribed it eno1 it mrna into neuroblastoma cells induces cell death |
| url | http://www.biomedcentral.com/1471-2407/5/161 |
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