A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours
Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study w...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1591931 |
| _version_ | 1818648106839834624 |
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| author | Nur Topyalin Metin Budak Nurver Ozbay Mustafa Yildiz Tuncay Kaner Abdullah Aydin Ahmet Ferruh Gezen |
| author_facet | Nur Topyalin Metin Budak Nurver Ozbay Mustafa Yildiz Tuncay Kaner Abdullah Aydin Ahmet Ferruh Gezen |
| author_sort | Nur Topyalin |
| collection | DOAJ |
| description | Survivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p < 0.01), suggesting that Ki-67 is a more sensitive marker compared to survivin. |
| first_indexed | 2024-12-17T01:13:10Z |
| format | Article |
| id | doaj.art-b7855905a801412081d2b0cc81946297 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-17T01:13:10Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-b7855905a801412081d2b0cc819462972022-12-21T22:09:05ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-0133150450910.1080/13102818.2019.15919311591931A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumoursNur Topyalin0Metin Budak1Nurver Ozbay2Mustafa Yildiz3Tuncay Kaner4Abdullah Aydin5Ahmet Ferruh Gezen6Neurosurgery Clinic, Istanbul Medeniyet University, Goztepe Training and Research HospitalTrakya UniversityIstanbul Medeniyet University, Goztepe Training and Research HospitalTrakya UniversityNeurosurgery Clinic, Istanbul Medeniyet University, Goztepe Training and Research HospitalNeurosurgery Clinic, Istanbul Medeniyet University, Goztepe Training and Research HospitalNeurosurgery Clinic, Istanbul Medeniyet University, Goztepe Training and Research HospitalSurvivin is a bifunctional protein which regulates cell division and inhibits apoptosis. Survivin is a member of the inhibitor of apoptosis protein (IAP) family. Expression of survivin has been shown to be responsible for apoptosis and resistance to ionizing radiation. The aim of the present study was to investigate the association between −31 G/C promoter polymorphism, survivin protein and glial tumour grading, and to compare survivin versus Ki-67 as a marker. In this study, DNA was isolated from paraffin-embedded sections of 29 patients diagnosed with glial tumours. Survivin gene promoter −31 G/C polymorphism was investigated using PCR-RFLP. For the analysis, 10 µm sections were stained with survivin protein and Ki-67 antibody. Immunohistochemical staining was performed. Survivin showed a positive correlation with Ki-67 (r = 0.604; p = 0.001). The tumour grades correlated with survivin; however, the relationship was not statistically significant (r = 0.345; p > 0.05). We found a significant correlation between tumour grades and Ki-67 (r = 0.663; p < 0.01), suggesting that Ki-67 is a more sensitive marker compared to survivin.http://dx.doi.org/10.1080/13102818.2019.1591931glial tumoursurvivinpolymorphismki-67 |
| spellingShingle | Nur Topyalin Metin Budak Nurver Ozbay Mustafa Yildiz Tuncay Kaner Abdullah Aydin Ahmet Ferruh Gezen A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours Biotechnology & Biotechnological Equipment glial tumour survivin polymorphism ki-67 |
| title | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
| title_full | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
| title_fullStr | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
| title_full_unstemmed | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
| title_short | A comparative histopathological and immunohistochemical study of Survivin and Ki-67 proteins in glial tumours |
| title_sort | comparative histopathological and immunohistochemical study of survivin and ki 67 proteins in glial tumours |
| topic | glial tumour survivin polymorphism ki-67 |
| url | http://dx.doi.org/10.1080/13102818.2019.1591931 |
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