Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>)
In this study, post-larval coho salmon <i>Oncorhynchus kisutch</i> (initial weight 0.37 ± 0.03 g) were fed with 6 experimental diets with increasing manganese (Mn) content (2.4, 8.5, 14.8, 19.8, 24.6, and 33.7 mg kg<sup>−1</sup>) for 12 weeks. Our results indicated that the f...
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MDPI AG
2023-04-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/8/1310 |
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| author | Dongwu Liu Lingyao Li Qin Zhang Hairui Yu |
| author_facet | Dongwu Liu Lingyao Li Qin Zhang Hairui Yu |
| author_sort | Dongwu Liu |
| collection | DOAJ |
| description | In this study, post-larval coho salmon <i>Oncorhynchus kisutch</i> (initial weight 0.37 ± 0.03 g) were fed with 6 experimental diets with increasing manganese (Mn) content (2.4, 8.5, 14.8, 19.8, 24.6, and 33.7 mg kg<sup>−1</sup>) for 12 weeks. Our results indicated that the feed conversion rate (FCR), specific growth rate (SGR), condition factor (CF), crude protein, moisture, crude lipid, ash, whole-body Mn, and vertebral Mn were affected by the elevation of Mn content in the diet. The activities of hepatic GSH-PX, Mn-SOD, and CAT were enhanced with increasing Mn content in the diet and reached the highest value at 19.8 mg kg<sup>−1</sup> Mn. However, the level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anion (O<sub>2</sub><sup>·−</sup>), and malondialdehyde (MDA) was reduced with increasing Mn content in the diet. In addition, the activity of hepatic lipase (HL) and lipoprotein lipase (LPL) was increased with the elevation of dietary Mn content and reached a peak value at 14.8 mg kg<sup>−1</sup> Mn. The activity of fatty acid synthetase (FAS) and the content of nonesterified fatty acid (NEFA) were increased following the elevation of Mn content from 2.4 to 19.8 mg kg<sup>−1</sup> in the diet. The results indicated that the appropriate dietary Mn supplementation improved the feeding efficiency, lipid metabolism, and antioxidant capacity of coho salmon. The dietary Mn requirement for post-larval coho salmon was 17.35 mg kg<sup>−1</sup> and 19.75 mg kg<sup>−1</sup> based on the SGR and FCR, respectively. An optimal dietary level of Mn enhances hepatic lipid metabolism, and the signaling pathway of PI3K/AKT/mTOR may be involved in regulating the activity of enzymes related to lipid metabolism. |
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| spelling | doaj.art-b785ff73fc9a44ac9962101e6d8e20e82023-11-17T17:59:57ZengMDPI AGAnimals2076-26152023-04-01138131010.3390/ani13081310Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>)Dongwu Liu0Lingyao Li1Qin Zhang2Hairui Yu3School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255049, ChinaWeifang Key Laboratory of Coho Salmon Culturing Facility Engineering, Institute of Modern Facility Fisheries, College of Biology and Oceanography, Weifang University, Weifang 261061, ChinaGuangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, School of Marine Science and Biotechnology, Guangxi Minzu University, Nanning 530008, ChinaWeifang Key Laboratory of Coho Salmon Culturing Facility Engineering, Institute of Modern Facility Fisheries, College of Biology and Oceanography, Weifang University, Weifang 261061, ChinaIn this study, post-larval coho salmon <i>Oncorhynchus kisutch</i> (initial weight 0.37 ± 0.03 g) were fed with 6 experimental diets with increasing manganese (Mn) content (2.4, 8.5, 14.8, 19.8, 24.6, and 33.7 mg kg<sup>−1</sup>) for 12 weeks. Our results indicated that the feed conversion rate (FCR), specific growth rate (SGR), condition factor (CF), crude protein, moisture, crude lipid, ash, whole-body Mn, and vertebral Mn were affected by the elevation of Mn content in the diet. The activities of hepatic GSH-PX, Mn-SOD, and CAT were enhanced with increasing Mn content in the diet and reached the highest value at 19.8 mg kg<sup>−1</sup> Mn. However, the level of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), superoxide anion (O<sub>2</sub><sup>·−</sup>), and malondialdehyde (MDA) was reduced with increasing Mn content in the diet. In addition, the activity of hepatic lipase (HL) and lipoprotein lipase (LPL) was increased with the elevation of dietary Mn content and reached a peak value at 14.8 mg kg<sup>−1</sup> Mn. The activity of fatty acid synthetase (FAS) and the content of nonesterified fatty acid (NEFA) were increased following the elevation of Mn content from 2.4 to 19.8 mg kg<sup>−1</sup> in the diet. The results indicated that the appropriate dietary Mn supplementation improved the feeding efficiency, lipid metabolism, and antioxidant capacity of coho salmon. The dietary Mn requirement for post-larval coho salmon was 17.35 mg kg<sup>−1</sup> and 19.75 mg kg<sup>−1</sup> based on the SGR and FCR, respectively. An optimal dietary level of Mn enhances hepatic lipid metabolism, and the signaling pathway of PI3K/AKT/mTOR may be involved in regulating the activity of enzymes related to lipid metabolism.https://www.mdpi.com/2076-2615/13/8/1310manganese requirementproximate compositionfatty acid synthetasemTOR signalingfeeding efficiency |
| spellingShingle | Dongwu Liu Lingyao Li Qin Zhang Hairui Yu Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) Animals manganese requirement proximate composition fatty acid synthetase mTOR signaling feeding efficiency |
| title | Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) |
| title_full | Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) |
| title_fullStr | Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) |
| title_full_unstemmed | Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) |
| title_short | Effect of Dietary Manganese on the Growth Performance, Lipid Metabolism, and Antioxidant Capacity in the Post-Larval Coho Salmon (<i>Oncorhynchus kisutch</i>) |
| title_sort | effect of dietary manganese on the growth performance lipid metabolism and antioxidant capacity in the post larval coho salmon i oncorhynchus kisutch i |
| topic | manganese requirement proximate composition fatty acid synthetase mTOR signaling feeding efficiency |
| url | https://www.mdpi.com/2076-2615/13/8/1310 |
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