Microsatellite characterization of Cimarron Uruguayo dogs
Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Genética
2011-01-01
|
Series: | Genetics and Molecular Biology |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028 |
_version_ | 1828414484983578624 |
---|---|
author | Rosa Gagliardi Llambí Silvia Cristina García María Victoria Arruga |
author_facet | Rosa Gagliardi Llambí Silvia Cristina García María Victoria Arruga |
author_sort | Rosa Gagliardi |
collection | DOAJ |
description | Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values > 0.7, in agreement with results for other canine breeds. The PE values for the markers were within the ranges previously described and were generally greater for microsatellites with higher PIC values. The heterozygosity value (0.649) was considered high since only nine microsatellites were analyzed. Compared with data for other breeds, the results obtained here indicate that Cimarron Uruguayo dogs have high genetic diversity. |
first_indexed | 2024-12-10T13:27:12Z |
format | Article |
id | doaj.art-b786f001d6564742b38343df54b7e345 |
institution | Directory Open Access Journal |
issn | 1415-4757 1678-4685 |
language | English |
last_indexed | 2024-12-10T13:27:12Z |
publishDate | 2011-01-01 |
publisher | Sociedade Brasileira de Genética |
record_format | Article |
series | Genetics and Molecular Biology |
spelling | doaj.art-b786f001d6564742b38343df54b7e3452022-12-22T01:47:09ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-01341165168Microsatellite characterization of Cimarron Uruguayo dogsRosa GagliardiLlambí SilviaCristina GarcíaMaría Victoria ArrugaVarious genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values > 0.7, in agreement with results for other canine breeds. The PE values for the markers were within the ranges previously described and were generally greater for microsatellites with higher PIC values. The heterozygosity value (0.649) was considered high since only nine microsatellites were analyzed. Compared with data for other breeds, the results obtained here indicate that Cimarron Uruguayo dogs have high genetic diversity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028Cimarron Uruguayo doggenetic characterizationmicrosatellites |
spellingShingle | Rosa Gagliardi Llambí Silvia Cristina García María Victoria Arruga Microsatellite characterization of Cimarron Uruguayo dogs Genetics and Molecular Biology Cimarron Uruguayo dog genetic characterization microsatellites |
title | Microsatellite characterization of Cimarron Uruguayo dogs |
title_full | Microsatellite characterization of Cimarron Uruguayo dogs |
title_fullStr | Microsatellite characterization of Cimarron Uruguayo dogs |
title_full_unstemmed | Microsatellite characterization of Cimarron Uruguayo dogs |
title_short | Microsatellite characterization of Cimarron Uruguayo dogs |
title_sort | microsatellite characterization of cimarron uruguayo dogs |
topic | Cimarron Uruguayo dog genetic characterization microsatellites |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028 |
work_keys_str_mv | AT rosagagliardi microsatellitecharacterizationofcimarronuruguayodogs AT llambisilvia microsatellitecharacterizationofcimarronuruguayodogs AT cristinagarcia microsatellitecharacterizationofcimarronuruguayodogs AT mariavictoriaarruga microsatellitecharacterizationofcimarronuruguayodogs |