Microsatellite characterization of Cimarron Uruguayo dogs

Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine...

Full description

Bibliographic Details
Main Authors: Rosa Gagliardi, Llambí Silvia, Cristina García, María Victoria Arruga
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2011-01-01
Series:Genetics and Molecular Biology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028
_version_ 1828414484983578624
author Rosa Gagliardi
Llambí Silvia
Cristina García
María Victoria Arruga
author_facet Rosa Gagliardi
Llambí Silvia
Cristina García
María Victoria Arruga
author_sort Rosa Gagliardi
collection DOAJ
description Various genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values > 0.7, in agreement with results for other canine breeds. The PE values for the markers were within the ranges previously described and were generally greater for microsatellites with higher PIC values. The heterozygosity value (0.649) was considered high since only nine microsatellites were analyzed. Compared with data for other breeds, the results obtained here indicate that Cimarron Uruguayo dogs have high genetic diversity.
first_indexed 2024-12-10T13:27:12Z
format Article
id doaj.art-b786f001d6564742b38343df54b7e345
institution Directory Open Access Journal
issn 1415-4757
1678-4685
language English
last_indexed 2024-12-10T13:27:12Z
publishDate 2011-01-01
publisher Sociedade Brasileira de Genética
record_format Article
series Genetics and Molecular Biology
spelling doaj.art-b786f001d6564742b38343df54b7e3452022-12-22T01:47:09ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852011-01-01341165168Microsatellite characterization of Cimarron Uruguayo dogsRosa GagliardiLlambí SilviaCristina GarcíaMaría Victoria ArrugaVarious genetic markers, including microsatellites, have been used to analyze the genetic polymorphism and heterozygosity in canine breeds. In this work, we used nine microsatellite markers to investigate the genetic variability in Cimarron Uruguayo dogs, the only officially recognized native canine breed in Uruguay. DNA from 30 Cimarron Uruguayo dogs from northeastern and southern Uruguay was analyzed. The allelic frequencies for each micro-satellite, the genetic variability and the consanguinity were calculated, as were the polymorphic information content (PIC) and the probability of exclusion (PE). All of the microsatellites studied were polymorphic. FH 2361, FH 2305 and PEZ 03 were the most informative, with PIC values > 0.7, in agreement with results for other canine breeds. The PE values for the markers were within the ranges previously described and were generally greater for microsatellites with higher PIC values. The heterozygosity value (0.649) was considered high since only nine microsatellites were analyzed. Compared with data for other breeds, the results obtained here indicate that Cimarron Uruguayo dogs have high genetic diversity.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028Cimarron Uruguayo doggenetic characterizationmicrosatellites
spellingShingle Rosa Gagliardi
Llambí Silvia
Cristina García
María Victoria Arruga
Microsatellite characterization of Cimarron Uruguayo dogs
Genetics and Molecular Biology
Cimarron Uruguayo dog
genetic characterization
microsatellites
title Microsatellite characterization of Cimarron Uruguayo dogs
title_full Microsatellite characterization of Cimarron Uruguayo dogs
title_fullStr Microsatellite characterization of Cimarron Uruguayo dogs
title_full_unstemmed Microsatellite characterization of Cimarron Uruguayo dogs
title_short Microsatellite characterization of Cimarron Uruguayo dogs
title_sort microsatellite characterization of cimarron uruguayo dogs
topic Cimarron Uruguayo dog
genetic characterization
microsatellites
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100028
work_keys_str_mv AT rosagagliardi microsatellitecharacterizationofcimarronuruguayodogs
AT llambisilvia microsatellitecharacterizationofcimarronuruguayodogs
AT cristinagarcia microsatellitecharacterizationofcimarronuruguayodogs
AT mariavictoriaarruga microsatellitecharacterizationofcimarronuruguayodogs