<i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice
Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases and encompasses non-alcoholic steatosis, steatohepatitis, and fibrosis. <i>Sanguisorba officinalis</i> L. (SO) roots have traditionally been used for their antioxidant properties and have beneficial ef...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/2072-6643/15/17/3779 |
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| author | Yunseong Nam Myungsuk Kim Saruul Erdenebileg Kwang Hyun Cha Da Hye Ryu Ho Youn Kim Su Hyeon Lee Je Hyeong Jung Chu Won Nho |
| author_facet | Yunseong Nam Myungsuk Kim Saruul Erdenebileg Kwang Hyun Cha Da Hye Ryu Ho Youn Kim Su Hyeon Lee Je Hyeong Jung Chu Won Nho |
| author_sort | Yunseong Nam |
| collection | DOAJ |
| description | Non-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases and encompasses non-alcoholic steatosis, steatohepatitis, and fibrosis. <i>Sanguisorba officinalis</i> L. (SO) roots have traditionally been used for their antioxidant properties and have beneficial effects on metabolic disorders, including diabetes and obesity. However, its effects on hepatic steatosis and fibrosis remain unclear. In this study, we explored the effects of a 95% ethanolic SO extract (SOEE) on NAFLD and fibrosis in vivo and in vitro. The SOEE was orally administered to C57BL/6J mice fed a choline-deficient, L-amino-acid-defined, high-fat diet for 10 weeks. The SOEE inhibited hepatic steatosis by modulating hepatic malondialdehyde levels and the expression of oxidative stress-associated genes, regulating fatty-acid-oxidation-related genes, and inhibiting the expression of genes that are responsible for fibrosis. The SOEE suppressed the deposition of extracellular matrix hydroxyproline and mRNA expression of fibrosis-associated genes. The SOEE decreased the expression of fibrosis-related genes in vitro by inhibiting SMAD2/3 phosphorylation. Furthermore, the SOEE restored the gut microbial diversity and modulated specific bacterial genera associated with NAFLD and fibrosis. This study suggests that SOEE might be the potential candidate for inhibiting hepatic steatosis and fibrosis by modulating oxidative stress, fatty acid oxidation, and gut microbiota composition. |
| first_indexed | 2024-03-10T23:15:42Z |
| format | Article |
| id | doaj.art-b7872a7acdd04da9915da729b42cb165 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T23:15:42Z |
| publishDate | 2023-08-01 |
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| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b7872a7acdd04da9915da729b42cb1652023-11-19T08:39:00ZengMDPI AGNutrients2072-66432023-08-011517377910.3390/nu15173779<i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced MiceYunseong Nam0Myungsuk Kim1Saruul Erdenebileg2Kwang Hyun Cha3Da Hye Ryu4Ho Youn Kim5Su Hyeon Lee6Je Hyeong Jung7Chu Won Nho8Division of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung 25451, Republic of KoreaDivision of Bio-Medical Science and Technology, KIST School, University of Science and Technology (UST), Seoul 02792, Republic of KoreaNon-alcoholic fatty liver disease (NAFLD) is a leading cause of chronic liver diseases and encompasses non-alcoholic steatosis, steatohepatitis, and fibrosis. <i>Sanguisorba officinalis</i> L. (SO) roots have traditionally been used for their antioxidant properties and have beneficial effects on metabolic disorders, including diabetes and obesity. However, its effects on hepatic steatosis and fibrosis remain unclear. In this study, we explored the effects of a 95% ethanolic SO extract (SOEE) on NAFLD and fibrosis in vivo and in vitro. The SOEE was orally administered to C57BL/6J mice fed a choline-deficient, L-amino-acid-defined, high-fat diet for 10 weeks. The SOEE inhibited hepatic steatosis by modulating hepatic malondialdehyde levels and the expression of oxidative stress-associated genes, regulating fatty-acid-oxidation-related genes, and inhibiting the expression of genes that are responsible for fibrosis. The SOEE suppressed the deposition of extracellular matrix hydroxyproline and mRNA expression of fibrosis-associated genes. The SOEE decreased the expression of fibrosis-related genes in vitro by inhibiting SMAD2/3 phosphorylation. Furthermore, the SOEE restored the gut microbial diversity and modulated specific bacterial genera associated with NAFLD and fibrosis. This study suggests that SOEE might be the potential candidate for inhibiting hepatic steatosis and fibrosis by modulating oxidative stress, fatty acid oxidation, and gut microbiota composition.https://www.mdpi.com/2072-6643/15/17/3779<i>Sanguisorba officinalis</i> L.steatosisfibrosisgut microbiotacholine-deficientL-amino acid-defined |
| spellingShingle | Yunseong Nam Myungsuk Kim Saruul Erdenebileg Kwang Hyun Cha Da Hye Ryu Ho Youn Kim Su Hyeon Lee Je Hyeong Jung Chu Won Nho <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice Nutrients <i>Sanguisorba officinalis</i> L. steatosis fibrosis gut microbiota choline-deficient L-amino acid-defined |
| title | <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice |
| title_full | <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice |
| title_fullStr | <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice |
| title_full_unstemmed | <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice |
| title_short | <i>Sanguisorba officinalis</i> L. Ameliorates Hepatic Steatosis and Fibrosis by Modulating Oxidative Stress, Fatty Acid Oxidation, and Gut Microbiota in CDAHFD-Induced Mice |
| title_sort | i sanguisorba officinalis i l ameliorates hepatic steatosis and fibrosis by modulating oxidative stress fatty acid oxidation and gut microbiota in cdahfd induced mice |
| topic | <i>Sanguisorba officinalis</i> L. steatosis fibrosis gut microbiota choline-deficient L-amino acid-defined |
| url | https://www.mdpi.com/2072-6643/15/17/3779 |
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