Severe acute respiratory syndrome coronavirus 2 spike ARTIC amplicon 76 dropout in relation to primers, viral load, and variants
Background/aim In January 2020, the ARTIC Network designed 98 pairs of primers divided into two pools for targeting amplification of the severe acute respiratory syndrome coronavirus 2 genome by multiplex PCR. However, in using this protocol, several users, including our team at Emerging Pathogens I...
Main Authors: | Maha G Haggag, Massimiliano S Tagliamonte, Melanie N Cash, Carla N Mavian |
---|---|
Format: | Article |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2022-01-01
|
Series: | Journal of the Arab Society for Medical Research |
Subjects: | |
Online Access: | http://www.new.asmr.eg.net/article.asp?issn=1687-4293;year=2022;volume=17;issue=2;spage=180;epage=187;aulast=Haggag |
Similar Items
-
Variation at Spike position 142 in SARS-CoV-2 Delta genomes is a technical artifact caused by dropout of a sequencing amplicon [version 1; peer review: 2 approved]
by: Jeffrey C. Barrett, et al.
Published: (2021-11-01) -
A Novel Tiled Amplicon Sequencing Assay Targeting the Tomato Brown Rugose Fruit Virus (ToBRFV) Genome Reveals Widespread Distribution in Municipal Wastewater Treatment Systems in the Province of Ontario, Canada
by: Delaney Nash, et al.
Published: (2024-03-01) -
High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in
by: Tejali Naik, et al.
Published: (2023-03-01) -
Liturgical articals and ornaments kept in the sacristy of St Mary’s Church in Cracow in XIV and XV centuries
by: Elżbieta Piwowarczyk
Published: (2002-12-01) -
SARS-CoV-2 variants of concern and spike protein mutational dynamics in a Swedish cohort during 2021, studied by Nanopore sequencing
by: Steinar Mannsverk, et al.
Published: (2022-10-01)