The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages
The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophag...
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| Format: | Article |
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2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Aleksey M. Krasnyi Alsu A. Sadekova Tatyana Y. Smolnova Vyacheslav V. Chursin Natalya A. Buralkina Vladimir D. Chuprynin Ekaterina Yarotskaya Stanislav V. Pavlovich Gennadiy T. Sukhikh |
| author_facet | Aleksey M. Krasnyi Alsu A. Sadekova Tatyana Y. Smolnova Vyacheslav V. Chursin Natalya A. Buralkina Vladimir D. Chuprynin Ekaterina Yarotskaya Stanislav V. Pavlovich Gennadiy T. Sukhikh |
| author_sort | Aleksey M. Krasnyi |
| collection | DOAJ |
| description | The aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (<i>p</i> = 0.007, <i>p</i> = 0.009, <i>p</i> = 0.002, <i>p</i> = 0.008, respectively). At the same time, there was a noted increase in the CD10 protease expression by peritoneal macrophages (<i>p</i> = 0.044). Correlation analysis showed a positive correlation of ghrelin and GLP-1 levels with CD86 macrophage expression (<i>p</i> = 0.044, <i>p</i> = 0.022, respectively) in the study group; a positive correlation was also found between the levels of GLP-1, glucagon, and visfatin with CD26 macrophage expression (<i>p</i> = 0.041, <i>p</i> = 0.048, <i>p</i> = 0.015, respectively) in PF. No correlations were found in the control group. These results indicate that a decrease in the levels of ghrelin, GLP-1, glucagon, and visfatin in PF may contribute to endometriosis development through their impact on the expression of pro-inflammatory markers of PF macrophages. |
| first_indexed | 2024-03-09T23:48:04Z |
| format | Article |
| id | doaj.art-b788635a18e74f79a9ee41824b82cbfa |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:48:04Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b788635a18e74f79a9ee41824b82cbfa2023-11-23T16:40:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181036110.3390/ijms231810361The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the MacrophagesAleksey M. Krasnyi0Alsu A. Sadekova1Tatyana Y. Smolnova2Vyacheslav V. Chursin3Natalya A. Buralkina4Vladimir D. Chuprynin5Ekaterina Yarotskaya6Stanislav V. Pavlovich7Gennadiy T. Sukhikh8National Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaNational Medical Research Center for Obstetrics, Gynecology and Perinatology of Ministry of Healthcare of Russian Federation, Ac. Oparina Str. 4, 117997 Moscow, RussiaThe aim of this study was to evaluate the levels of ten energy metabolism factors: C-peptide, ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1 (total), resistin, and visfatin, and to determine the expression of GLP1R receptors, CD10, CD26 proteases, and pro-inflammatory marker CD86 by macrophages in the peritoneal fluid (PF) in patients with endometriosis. The study included 54 women with endometriosis and a control group of 30 women with uterine myoma without signs of endometriosis. The levels of factors in PF were assessed by a multiplex method. Expression of GLP1R receptors, CD10, CD26 proteases, and CD86 by macrophages was evaluated using flow cytometry. It was found that in women with endometriosis, the concentrations of ghrelin, GLP-1, glucagon, and visfatin in PF were reduced (<i>p</i> = 0.007, <i>p</i> = 0.009, <i>p</i> = 0.002, <i>p</i> = 0.008, respectively). At the same time, there was a noted increase in the CD10 protease expression by peritoneal macrophages (<i>p</i> = 0.044). Correlation analysis showed a positive correlation of ghrelin and GLP-1 levels with CD86 macrophage expression (<i>p</i> = 0.044, <i>p</i> = 0.022, respectively) in the study group; a positive correlation was also found between the levels of GLP-1, glucagon, and visfatin with CD26 macrophage expression (<i>p</i> = 0.041, <i>p</i> = 0.048, <i>p</i> = 0.015, respectively) in PF. No correlations were found in the control group. These results indicate that a decrease in the levels of ghrelin, GLP-1, glucagon, and visfatin in PF may contribute to endometriosis development through their impact on the expression of pro-inflammatory markers of PF macrophages.https://www.mdpi.com/1422-0067/23/18/10361endometriosisperitoneal fluidmacrophagesghrelinGLP-1glucagon |
| spellingShingle | Aleksey M. Krasnyi Alsu A. Sadekova Tatyana Y. Smolnova Vyacheslav V. Chursin Natalya A. Buralkina Vladimir D. Chuprynin Ekaterina Yarotskaya Stanislav V. Pavlovich Gennadiy T. Sukhikh The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages International Journal of Molecular Sciences endometriosis peritoneal fluid macrophages ghrelin GLP-1 glucagon |
| title | The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages |
| title_full | The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages |
| title_fullStr | The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages |
| title_full_unstemmed | The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages |
| title_short | The Levels of Ghrelin, Glucagon, Visfatin and Glp-1 Are Decreased in the Peritoneal Fluid of Women with Endometriosis along with the Increased Expression of the CD10 Protease by the Macrophages |
| title_sort | levels of ghrelin glucagon visfatin and glp 1 are decreased in the peritoneal fluid of women with endometriosis along with the increased expression of the cd10 protease by the macrophages |
| topic | endometriosis peritoneal fluid macrophages ghrelin GLP-1 glucagon |
| url | https://www.mdpi.com/1422-0067/23/18/10361 |
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