To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved]
Background: The main factors that affect the success of an endodontic infection are effective cleaning and shaping of the root canal system including complete disinfection by using chemical irrigating solutions and obturation with an endodontic sealer to provide a fluid-tight seal. Using rotary and...
| Main Authors: | , , , |
|---|---|
| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
F1000 Research Ltd
2023-02-01
|
| Series: | F1000Research |
| Subjects: | |
| Acceso en liña: | https://f1000research.com/articles/11-1561/v2 |
| _version_ | 1828001029957877760 |
|---|---|
| author | Shivangi Shekhar P. Laxmish Mallya Vasudev Ballal Ramya Shenoy |
| author_facet | Shivangi Shekhar P. Laxmish Mallya Vasudev Ballal Ramya Shenoy |
| author_sort | Shivangi Shekhar |
| collection | DOAJ |
| description | Background: The main factors that affect the success of an endodontic infection are effective cleaning and shaping of the root canal system including complete disinfection by using chemical irrigating solutions and obturation with an endodontic sealer to provide a fluid-tight seal. Using rotary and hand instruments for shaping and cleaning the root canal along with irrigants produces a smear layer on the surface of the root dentin affecting the penetration of the endodontic sealer into the dentinal tubules. This smear is difficult to remove with the use of only endodontic irrigants, hence, chelating agents were introduced in adjunct with irrigating solutions for irrigation protocol for effective removal of smear layer which affect the penetration of endodontic sealers into the dentinal tubules. Methods: 32 mandibular premolar teeth were used. The biomechanical preparation was done till Protaper F3 size. Irrigation was done with 2.5 mL sodium hypochlorite (NaOCl) solution after each instrumentation change for 1 min. Samples were then divided into 4 groups according to the irrigating solution used as the final rinse used with passive ultrasonic agitation. The groups were: Group I: 5 ml of saline, Group II: 5ml of 17% ethylenediaminetetraacetic acid, Group III: 5 ml of 10% citric acid, Group IV: 5 ml of 7% maleic acid each for one minute. All the canals were obturated with BioRoot tmRCS with gutta-percha using the ultrasonic condensation technique. For staining the samples for Confocal LASER microscopy, BioRoot tmRCS was mixed with Rhodamine B dye. Results: The maximum penetration of bio-ceramic sealer was observed in the coronal region. At the apical third, the maximum sealer penetration was seen with 7% maleic acid. Conclusions: Maximum sealer penetration was seen in the coronal section followed by the middle and apical section. Maximum sealer penetration was seen with 7 % maleic acid at the apical third. |
| first_indexed | 2024-04-10T06:23:07Z |
| format | Article |
| id | doaj.art-b7890d634b6c4912a8fb00f97ec0fb5c |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-04-10T06:23:07Z |
| publishDate | 2023-02-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-b7890d634b6c4912a8fb00f97ec0fb5c2023-03-02T01:00:01ZengF1000 Research LtdF1000Research2046-14022023-02-0111144757To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved]Shivangi Shekhar0https://orcid.org/0000-0003-3944-2845P. Laxmish Mallya1https://orcid.org/0000-0003-3975-040XVasudev Ballal2https://orcid.org/0000-0002-2208-9443Ramya Shenoy3Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 575001, IndiaDepartment of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal, Karnataka, 575001, IndiaConservative Dentistry and Endodontics, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 576104, IndiaDepartment of Public Health Dentistry, Manipal College of Dental Sciences, Mangalore, Manipal Academy of Higher Education, Manipal, Karnataka, 575001, IndiaBackground: The main factors that affect the success of an endodontic infection are effective cleaning and shaping of the root canal system including complete disinfection by using chemical irrigating solutions and obturation with an endodontic sealer to provide a fluid-tight seal. Using rotary and hand instruments for shaping and cleaning the root canal along with irrigants produces a smear layer on the surface of the root dentin affecting the penetration of the endodontic sealer into the dentinal tubules. This smear is difficult to remove with the use of only endodontic irrigants, hence, chelating agents were introduced in adjunct with irrigating solutions for irrigation protocol for effective removal of smear layer which affect the penetration of endodontic sealers into the dentinal tubules. Methods: 32 mandibular premolar teeth were used. The biomechanical preparation was done till Protaper F3 size. Irrigation was done with 2.5 mL sodium hypochlorite (NaOCl) solution after each instrumentation change for 1 min. Samples were then divided into 4 groups according to the irrigating solution used as the final rinse used with passive ultrasonic agitation. The groups were: Group I: 5 ml of saline, Group II: 5ml of 17% ethylenediaminetetraacetic acid, Group III: 5 ml of 10% citric acid, Group IV: 5 ml of 7% maleic acid each for one minute. All the canals were obturated with BioRoot tmRCS with gutta-percha using the ultrasonic condensation technique. For staining the samples for Confocal LASER microscopy, BioRoot tmRCS was mixed with Rhodamine B dye. Results: The maximum penetration of bio-ceramic sealer was observed in the coronal region. At the apical third, the maximum sealer penetration was seen with 7% maleic acid. Conclusions: Maximum sealer penetration was seen in the coronal section followed by the middle and apical section. Maximum sealer penetration was seen with 7 % maleic acid at the apical third.https://f1000research.com/articles/11-1561/v2Biofilms Citric Acid EDTA Maleic Acid Smear Layer.eng |
| spellingShingle | Shivangi Shekhar P. Laxmish Mallya Vasudev Ballal Ramya Shenoy To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] F1000Research Biofilms Citric Acid EDTA Maleic Acid Smear Layer. eng |
| title | To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] |
| title_full | To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] |
| title_fullStr | To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] |
| title_full_unstemmed | To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] |
| title_short | To evaluate and compare the effect of 17% EDTA, 10% citric acid, 7% maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy: an in vitro study [version 2; peer review: 2 approved] |
| title_sort | to evaluate and compare the effect of 17 edta 10 citric acid 7 maleic acid on the dentinal tubule penetration depth of bio ceramic root canal sealer using confocal laser scanning microscopy an in vitro study version 2 peer review 2 approved |
| topic | Biofilms Citric Acid EDTA Maleic Acid Smear Layer. eng |
| url | https://f1000research.com/articles/11-1561/v2 |
| work_keys_str_mv | AT shivangishekhar toevaluateandcomparetheeffectof17edta10citricacid7maleicacidonthedentinaltubulepenetrationdepthofbioceramicrootcanalsealerusingconfocallaserscanningmicroscopyaninvitrostudyversion2peerreview2approved AT plaxmishmallya toevaluateandcomparetheeffectof17edta10citricacid7maleicacidonthedentinaltubulepenetrationdepthofbioceramicrootcanalsealerusingconfocallaserscanningmicroscopyaninvitrostudyversion2peerreview2approved AT vasudevballal toevaluateandcomparetheeffectof17edta10citricacid7maleicacidonthedentinaltubulepenetrationdepthofbioceramicrootcanalsealerusingconfocallaserscanningmicroscopyaninvitrostudyversion2peerreview2approved AT ramyashenoy toevaluateandcomparetheeffectof17edta10citricacid7maleicacidonthedentinaltubulepenetrationdepthofbioceramicrootcanalsealerusingconfocallaserscanningmicroscopyaninvitrostudyversion2peerreview2approved |