Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project
Due to the COVID-19 pandemic, there has been a shift in focus towards controlling the spread of SARS-CoV-2, which has resulted in the neglect of traditional programs aimed at preventing healthcare-associated infections and combating antimicrobial resistance. The present work aims to characterize the...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/10/1551 |
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| author | Antonella Agodi Arturo Montineri Rosa Manuele Paola Noto Giuseppe Carpinteri Giacomo Castiglione Patrizia Grassi Antonio Lazzara Anna Rita Mattaliano Giuseppa Granvillano Claudia La Mastra Maria Clara La Rosa Andrea Maugeri Martina Barchitta |
| author_facet | Antonella Agodi Arturo Montineri Rosa Manuele Paola Noto Giuseppe Carpinteri Giacomo Castiglione Patrizia Grassi Antonio Lazzara Anna Rita Mattaliano Giuseppa Granvillano Claudia La Mastra Maria Clara La Rosa Andrea Maugeri Martina Barchitta |
| author_sort | Antonella Agodi |
| collection | DOAJ |
| description | Due to the COVID-19 pandemic, there has been a shift in focus towards controlling the spread of SARS-CoV-2, which has resulted in the neglect of traditional programs aimed at preventing healthcare-associated infections and combating antimicrobial resistance. The present work aims to characterize the colonization or infection with <i>Acinetobacter baumannii</i> of COVID-19 patients and to identify any clonality between different isolates. Specifically, data and resistance profiles of <i>A. baumannii</i> isolates were prospectively collected from patients recruited by the EPIRADIOCLINF project. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used for molecular typing. Overall, we analyzed 64 isolates of <i>A. baumannii</i> from 48 COVID-19 patients. According to our analysis, we have identified the spread of a clonally related isolate, referred to as B. The PFGE pattern B includes four subtypes: B1 (consisting of 37 strains), B2 (11), B3 (5), and B4 (2). Furthermore, in the isolates that were examined using MLST, the most observed sequence type was ST/281. In terms of resistance profiles, 59 out of the total isolates (92.2%) were found to be resistant to gentamicin, carbapenems, ciprofloxacin, and tobramycin. The isolation and identification of <i>A. baumannii</i> from COVID-19 patients, along with the high levels of transmission observed within the hospital setting, highlight the urgent need for the implementation of effective prevention and containment strategies. |
| first_indexed | 2024-03-10T21:29:40Z |
| format | Article |
| id | doaj.art-b78ac44b41a044c0984d730528ddbd06 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T21:29:40Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-b78ac44b41a044c0984d730528ddbd062023-11-19T15:27:05ZengMDPI AGAntibiotics2079-63822023-10-011210155110.3390/antibiotics12101551Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” ProjectAntonella Agodi0Arturo Montineri1Rosa Manuele2Paola Noto3Giuseppe Carpinteri4Giacomo Castiglione5Patrizia Grassi6Antonio Lazzara7Anna Rita Mattaliano8Giuseppa Granvillano9Claudia La Mastra10Maria Clara La Rosa11Andrea Maugeri12Martina Barchitta13Department of Medical and Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyAzienda Ospedaliero-Universitaria Policlinico AOUP “G. Rodolico-San Marco”, 95123 Catania, ItalyDepartment of Medical and Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, ItalyDepartment of Medical and Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, ItalyDepartment of Medical and Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, ItalyDepartment of Medical and Surgical Sciences and Advanced Technologies “GF Ingrassia”, University of Catania, 95123 Catania, ItalyDue to the COVID-19 pandemic, there has been a shift in focus towards controlling the spread of SARS-CoV-2, which has resulted in the neglect of traditional programs aimed at preventing healthcare-associated infections and combating antimicrobial resistance. The present work aims to characterize the colonization or infection with <i>Acinetobacter baumannii</i> of COVID-19 patients and to identify any clonality between different isolates. Specifically, data and resistance profiles of <i>A. baumannii</i> isolates were prospectively collected from patients recruited by the EPIRADIOCLINF project. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used for molecular typing. Overall, we analyzed 64 isolates of <i>A. baumannii</i> from 48 COVID-19 patients. According to our analysis, we have identified the spread of a clonally related isolate, referred to as B. The PFGE pattern B includes four subtypes: B1 (consisting of 37 strains), B2 (11), B3 (5), and B4 (2). Furthermore, in the isolates that were examined using MLST, the most observed sequence type was ST/281. In terms of resistance profiles, 59 out of the total isolates (92.2%) were found to be resistant to gentamicin, carbapenems, ciprofloxacin, and tobramycin. The isolation and identification of <i>A. baumannii</i> from COVID-19 patients, along with the high levels of transmission observed within the hospital setting, highlight the urgent need for the implementation of effective prevention and containment strategies.https://www.mdpi.com/2079-6382/12/10/1551SARS-CoV-2healthcare-associated infectionAMRclonal relationships |
| spellingShingle | Antonella Agodi Arturo Montineri Rosa Manuele Paola Noto Giuseppe Carpinteri Giacomo Castiglione Patrizia Grassi Antonio Lazzara Anna Rita Mattaliano Giuseppa Granvillano Claudia La Mastra Maria Clara La Rosa Andrea Maugeri Martina Barchitta Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project Antibiotics SARS-CoV-2 healthcare-associated infection AMR clonal relationships |
| title | Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project |
| title_full | Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project |
| title_fullStr | Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project |
| title_full_unstemmed | Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project |
| title_short | Molecular Typing and Resistance Profile of <i>Acinetobacter baumannii</i> Isolates during the COVID-19 Pandemic: Findings from the “EPIRADIOCLINF” Project |
| title_sort | molecular typing and resistance profile of i acinetobacter baumannii i isolates during the covid 19 pandemic findings from the epiradioclinf project |
| topic | SARS-CoV-2 healthcare-associated infection AMR clonal relationships |
| url | https://www.mdpi.com/2079-6382/12/10/1551 |
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