Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice
Introduction: Mesenchymal stem cells, such as adipose tissue-derived stem cells (Ad-SCs), are an appropriate source for cell therapy because of their differentiating properties and secretion of growth factors. This study was undertaken to evaluate the effect of transplantation of Ad-SCs in busulfan...
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| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Advances in Human Biology |
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| Online Access: | http://www.aihbonline.com/article.asp?issn=2321-8568;year=2023;volume=13;issue=2;spage=192;epage=198;aulast=Payehdar |
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| author | Arash Payehdar Seyed Ebrahim Hosseini Davood Mehrabani |
| author_facet | Arash Payehdar Seyed Ebrahim Hosseini Davood Mehrabani |
| author_sort | Arash Payehdar |
| collection | DOAJ |
| description | Introduction: Mesenchymal stem cells, such as adipose tissue-derived stem cells (Ad-SCs), are an appropriate source for cell therapy because of their differentiating properties and secretion of growth factors. This study was undertaken to evaluate the effect of transplantation of Ad-SCs in busulfan-induced azoospermic testes of mice, histomorphometric and histologically. Materials and Methods: Twenty-four adult male mice were randomly divided into four equal groups, including the control, azoospermia-induced, and two Ad-SCs transplanted groups sacrificed after 60 and 150 days, respectively. To induce azoospermia, animals received two doses of busulfan (10 mg/kg) intraperitoneally at 21 days' intervals. Ad-SCs were injected 35 days after the last busulfan injection into the efferent duct of the testis. The cells were isolated from the inguinal adipose tissue of two donor mice. The sacrificed mice were evaluated histomorphometric and histologically. Results: In histomorphometric evaluation, the spermatogenesis index of the Ad-SCs transplanted group after 60 and 150 days did not show any significant difference. At the same time, the index was significantly more than the control group. Most of the seminiferous tubules in the Ad-SCs group after 60 and 150 days were normal in morphology and spermatogenesis. Spermatogenesis was absent in the busulfan-induced group. Conclusion: Our findings based on a histological and histomorphometric study on transplantation of Ad-SCs in mice that recovered spermatogenesis in busulfan-induced azoospermic testes can be added to the literature on the effect of stem cells in the treatment of infertility. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2321-8568 2348-4691 |
| language | English |
| last_indexed | 2024-03-12T22:34:46Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advances in Human Biology |
| spelling | doaj.art-b78e4b622b0542519c34c4c8502100d02023-07-21T14:17:30ZengWolters Kluwer Medknow PublicationsAdvances in Human Biology2321-85682348-46912023-01-0113219219810.4103/aihb.aihb_161_21Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of miceArash PayehdarSeyed Ebrahim HosseiniDavood MehrabaniIntroduction: Mesenchymal stem cells, such as adipose tissue-derived stem cells (Ad-SCs), are an appropriate source for cell therapy because of their differentiating properties and secretion of growth factors. This study was undertaken to evaluate the effect of transplantation of Ad-SCs in busulfan-induced azoospermic testes of mice, histomorphometric and histologically. Materials and Methods: Twenty-four adult male mice were randomly divided into four equal groups, including the control, azoospermia-induced, and two Ad-SCs transplanted groups sacrificed after 60 and 150 days, respectively. To induce azoospermia, animals received two doses of busulfan (10 mg/kg) intraperitoneally at 21 days' intervals. Ad-SCs were injected 35 days after the last busulfan injection into the efferent duct of the testis. The cells were isolated from the inguinal adipose tissue of two donor mice. The sacrificed mice were evaluated histomorphometric and histologically. Results: In histomorphometric evaluation, the spermatogenesis index of the Ad-SCs transplanted group after 60 and 150 days did not show any significant difference. At the same time, the index was significantly more than the control group. Most of the seminiferous tubules in the Ad-SCs group after 60 and 150 days were normal in morphology and spermatogenesis. Spermatogenesis was absent in the busulfan-induced group. Conclusion: Our findings based on a histological and histomorphometric study on transplantation of Ad-SCs in mice that recovered spermatogenesis in busulfan-induced azoospermic testes can be added to the literature on the effect of stem cells in the treatment of infertility.http://www.aihbonline.com/article.asp?issn=2321-8568;year=2023;volume=13;issue=2;spage=192;epage=198;aulast=Payehdaradipose tissueazoospermiabusulfanmesenchymalmousestem cell |
| spellingShingle | Arash Payehdar Seyed Ebrahim Hosseini Davood Mehrabani Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice Advances in Human Biology adipose tissue azoospermia busulfan mesenchymal mouse stem cell |
| title | Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice |
| title_full | Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice |
| title_fullStr | Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice |
| title_full_unstemmed | Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice |
| title_short | Histomorphometric and histological assessments of transplantation of adipose tissue-derived stem cells in busulfan-induced azoospermic testis of mice |
| title_sort | histomorphometric and histological assessments of transplantation of adipose tissue derived stem cells in busulfan induced azoospermic testis of mice |
| topic | adipose tissue azoospermia busulfan mesenchymal mouse stem cell |
| url | http://www.aihbonline.com/article.asp?issn=2321-8568;year=2023;volume=13;issue=2;spage=192;epage=198;aulast=Payehdar |
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