It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate
This study aimed at valorizing digestate through <i>Trichoderma</i> spp. solid-state fermentation (SSF) to produce a potentially ameliorated fertilizer combined with fungal biomass as a value-added bioproduct. Plant-growth-promoting <i>Trichoderma atroviride</i> Ta13, <i&g...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/1/164 |
| _version_ | 1797491707789443072 |
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| author | Carlotta Alias Daniela Bulgari Emanuela Gobbi |
| author_facet | Carlotta Alias Daniela Bulgari Emanuela Gobbi |
| author_sort | Carlotta Alias |
| collection | DOAJ |
| description | This study aimed at valorizing digestate through <i>Trichoderma</i> spp. solid-state fermentation (SSF) to produce a potentially ameliorated fertilizer combined with fungal biomass as a value-added bioproduct. Plant-growth-promoting <i>Trichoderma atroviride</i> Ta13, <i>T. reesei</i> RUT-C30, <i>T. asperellum</i> R, and <i>T. harzianum</i> T-22 were tested on different SSF substrates: whole digestate (WD), digestate dried up with wood sawdust (SSF1), and digestate enriched with food waste and dried up with wood sawdust (SSF2). The fungal biomass was quantified by using a qPCR assay. The growth of the four <i>Trichoderma</i> spp. was only observed on the SSF2 substrate. The highest quantity of mycelium was produced by <i>T. reesei</i> RUT-30 (689.80 ± 80.53 mg/g substrate), followed by <i>T. atroviride</i> Ta13, and <i>T. asperellum</i> R (584.24 ± 13.36 and 444.79 ± 91.02 mg/g substrate). The germination of <i>Lepidium sativum</i> seeds was evaluated in order to assess the phytoxicity of the <i>Trichoderma</i>-enriched substrate. The treatments with 7.5% SSF2-R, 3.75% SSF2-T-22, and 1.8% SSF2-Ta13 equally enhanced the root elongation in comparison to the non-fermented SSF-2. This study demonstrated that digestate, mixed with agro-food waste, was able to support the cultivation of <i>Trichoderma</i> spp., paving the way to the valorization of fermented digestate as a proper biofertilizer. |
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| language | English |
| last_indexed | 2024-03-10T00:53:12Z |
| publishDate | 2022-01-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b792b6e733c14a6cb9dbb26e1f240b7e2023-11-23T14:48:18ZengMDPI AGMicroorganisms2076-26072022-01-0110116410.3390/microorganisms10010164It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural DigestateCarlotta Alias0Daniela Bulgari1Emanuela Gobbi2Agri-Food and Environmental Microbiology Platform (PiMiAA), Department of Molecular and Translational Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, ItalyAgri-Food and Environmental Microbiology Platform (PiMiAA), Department of Molecular and Translational Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, ItalyAgri-Food and Environmental Microbiology Platform (PiMiAA), Department of Molecular and Translational Medicine, University of Brescia, Viale Europa, 11, 25123 Brescia, ItalyThis study aimed at valorizing digestate through <i>Trichoderma</i> spp. solid-state fermentation (SSF) to produce a potentially ameliorated fertilizer combined with fungal biomass as a value-added bioproduct. Plant-growth-promoting <i>Trichoderma atroviride</i> Ta13, <i>T. reesei</i> RUT-C30, <i>T. asperellum</i> R, and <i>T. harzianum</i> T-22 were tested on different SSF substrates: whole digestate (WD), digestate dried up with wood sawdust (SSF1), and digestate enriched with food waste and dried up with wood sawdust (SSF2). The fungal biomass was quantified by using a qPCR assay. The growth of the four <i>Trichoderma</i> spp. was only observed on the SSF2 substrate. The highest quantity of mycelium was produced by <i>T. reesei</i> RUT-30 (689.80 ± 80.53 mg/g substrate), followed by <i>T. atroviride</i> Ta13, and <i>T. asperellum</i> R (584.24 ± 13.36 and 444.79 ± 91.02 mg/g substrate). The germination of <i>Lepidium sativum</i> seeds was evaluated in order to assess the phytoxicity of the <i>Trichoderma</i>-enriched substrate. The treatments with 7.5% SSF2-R, 3.75% SSF2-T-22, and 1.8% SSF2-Ta13 equally enhanced the root elongation in comparison to the non-fermented SSF-2. This study demonstrated that digestate, mixed with agro-food waste, was able to support the cultivation of <i>Trichoderma</i> spp., paving the way to the valorization of fermented digestate as a proper biofertilizer.https://www.mdpi.com/2076-2607/10/1/164agro-industrial wastefungal biomassqPCRcircular economybiofertilizer |
| spellingShingle | Carlotta Alias Daniela Bulgari Emanuela Gobbi It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate Microorganisms agro-industrial waste fungal biomass qPCR circular economy biofertilizer |
| title | It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate |
| title_full | It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate |
| title_fullStr | It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate |
| title_full_unstemmed | It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate |
| title_short | It Works! Organic-Waste-Assisted <i>Trichoderma</i> spp. Solid-State Fermentation on Agricultural Digestate |
| title_sort | it works organic waste assisted i trichoderma i spp solid state fermentation on agricultural digestate |
| topic | agro-industrial waste fungal biomass qPCR circular economy biofertilizer |
| url | https://www.mdpi.com/2076-2607/10/1/164 |
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