The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis
Cytokine-inducible SH2 domain-containing protein (CISH) is a member of the suppressor of cytokine signaling (SOCS) family of negative feedback regulators shown to play crucial roles in lymphoid cell development and function as well as appetite regulation. It has also been implicated in the control o...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/16/12757 |
| _version_ | 1797584435347980288 |
|---|---|
| author | Wasan Naser Saeed Maymand Daniel Dlugolenski Faiza Basheer Alister C. Ward |
| author_facet | Wasan Naser Saeed Maymand Daniel Dlugolenski Faiza Basheer Alister C. Ward |
| author_sort | Wasan Naser |
| collection | DOAJ |
| description | Cytokine-inducible SH2 domain-containing protein (CISH) is a member of the suppressor of cytokine signaling (SOCS) family of negative feedback regulators shown to play crucial roles in lymphoid cell development and function as well as appetite regulation. It has also been implicated in the control of signaling downstream of the receptors for the cytokines granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) in myeloid cells. To investigate the physiological role of CISH in myelopoiesis, mice deficient in CISH were analyzed basally and in response to administration of these cytokines. CISH knockout (KO) mice possessed basally elevated neutrophils in the blood, bone marrow, and spleen compared to wild-type (WT) mice. During GM-CSF-induced myelopoiesis, the frequency of neutrophils, myeloid dendritic cells (DCs), and CFU-M in the bone marrow was higher in the KO, as were the neutrophils and CFU-G in the spleen. In contrast, no differences were observed between KO and WT mice during G-CSF-induced myelopoiesis apart from an elevated frequency of CFU-G and CFU-M in the spleen. This work has identified a role for CISH in the negative regulation of granulopoiesis, including that mediated by GM-CSF. |
| first_indexed | 2024-03-10T23:52:38Z |
| format | Article |
| id | doaj.art-b79378e7f8fa4b22a06f92fd68c26150 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:52:38Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b79378e7f8fa4b22a06f92fd68c261502023-11-19T01:28:56ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124161275710.3390/ijms241612757The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated MyelopoiesisWasan Naser0Saeed Maymand1Daniel Dlugolenski2Faiza Basheer3Alister C. Ward4School of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaSchool of Medicine, Deakin University, Geelong, VIC 3216, AustraliaCytokine-inducible SH2 domain-containing protein (CISH) is a member of the suppressor of cytokine signaling (SOCS) family of negative feedback regulators shown to play crucial roles in lymphoid cell development and function as well as appetite regulation. It has also been implicated in the control of signaling downstream of the receptors for the cytokines granulocyte/macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) in myeloid cells. To investigate the physiological role of CISH in myelopoiesis, mice deficient in CISH were analyzed basally and in response to administration of these cytokines. CISH knockout (KO) mice possessed basally elevated neutrophils in the blood, bone marrow, and spleen compared to wild-type (WT) mice. During GM-CSF-induced myelopoiesis, the frequency of neutrophils, myeloid dendritic cells (DCs), and CFU-M in the bone marrow was higher in the KO, as were the neutrophils and CFU-G in the spleen. In contrast, no differences were observed between KO and WT mice during G-CSF-induced myelopoiesis apart from an elevated frequency of CFU-G and CFU-M in the spleen. This work has identified a role for CISH in the negative regulation of granulopoiesis, including that mediated by GM-CSF.https://www.mdpi.com/1422-0067/24/16/12757CISHcytokineG-CSFGM-CSFgranulopoiesismyelopoiesis |
| spellingShingle | Wasan Naser Saeed Maymand Daniel Dlugolenski Faiza Basheer Alister C. Ward The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis International Journal of Molecular Sciences CISH cytokine G-CSF GM-CSF granulopoiesis myelopoiesis |
| title | The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis |
| title_full | The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis |
| title_fullStr | The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis |
| title_full_unstemmed | The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis |
| title_short | The Role of Cytokine-Inducible SH2 Domain-Containing Protein (CISH) in the Regulation of Basal and Cytokine-Mediated Myelopoiesis |
| title_sort | role of cytokine inducible sh2 domain containing protein cish in the regulation of basal and cytokine mediated myelopoiesis |
| topic | CISH cytokine G-CSF GM-CSF granulopoiesis myelopoiesis |
| url | https://www.mdpi.com/1422-0067/24/16/12757 |
| work_keys_str_mv | AT wasannaser theroleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT saeedmaymand theroleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT danieldlugolenski theroleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT faizabasheer theroleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT alistercward theroleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT wasannaser roleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT saeedmaymand roleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT danieldlugolenski roleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT faizabasheer roleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis AT alistercward roleofcytokineinduciblesh2domaincontainingproteincishintheregulationofbasalandcytokinemediatedmyelopoiesis |