Synbiotic properties of Lactobacillus acidophilus M92

The scientific basis for the development of probiotics, prebiotics or their combination (synbiotics) stems from the knowledge that the gastrointestinal microflora is involved in protecting the host (man or animals) against colonization of the intestinal tract by non-indigenous microorganisms. The us...

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Main Authors: Jagoda Šušković, Blaženka Kos, Jadranka Frece, Sunčica Beluhan, Srećko Matošić
Format: Article
Language:English
Published: Croatian Dairy Union 2003-04-01
Series:Mljekarstvo
Subjects:
Online Access:http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4029
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author Jagoda Šušković
Blaženka Kos
Jadranka Frece
Sunčica Beluhan
Srećko Matošić
author_facet Jagoda Šušković
Blaženka Kos
Jadranka Frece
Sunčica Beluhan
Srećko Matošić
author_sort Jagoda Šušković
collection DOAJ
description The scientific basis for the development of probiotics, prebiotics or their combination (synbiotics) stems from the knowledge that the gastrointestinal microflora is involved in protecting the host (man or animals) against colonization of the intestinal tract by non-indigenous microorganisms. The use of probiotics (live beneficial microorganisms) and prebiotics (non-digestible oligosaccharides) as food/feed supplements beneficially affect the host by improving its intestinal microbial balance. Bacterial strain Lactobacillus acidophilus M92, which was selected for probiotic activity according to complex scientific in vitro selection criteria, is presented in this work. Accurate taxonomic identification of L. acidophilus M92 has been done by PCR method. The presence of S-layer proteins, hydrophobycity, autoaggregation, coaggregation and adhesion of L. acidophilus M92 to porcine ileal epithelial cells were determined. Because the concept of synbiotic became a part of probiotic concept in last few years, it was also investigated the ability of L. acidophilus M92 to assimilate the various kinds of prebiotic substrates (sorbitol, mannitol, lactulose, raffinose, oligofructose and inulin). The combination of probiotics and non-digestible carbohydrates (prebiotics) may be a way of stabilisation and/or improvement of the probiotic effect. Such synbiotics indicate a realistic way of using biological preparationsin the prevention of gastrointestinal diseases in humans and animals.
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spelling doaj.art-b7990ba87d7e40aaabdd70a2d0c2ece02022-12-21T18:36:59ZengCroatian Dairy UnionMljekarstvo0026-704X1846-40252003-04-0153283110Synbiotic properties of Lactobacillus acidophilus M92Jagoda ŠuškovićBlaženka KosJadranka FreceSunčica BeluhanSrećko MatošićThe scientific basis for the development of probiotics, prebiotics or their combination (synbiotics) stems from the knowledge that the gastrointestinal microflora is involved in protecting the host (man or animals) against colonization of the intestinal tract by non-indigenous microorganisms. The use of probiotics (live beneficial microorganisms) and prebiotics (non-digestible oligosaccharides) as food/feed supplements beneficially affect the host by improving its intestinal microbial balance. Bacterial strain Lactobacillus acidophilus M92, which was selected for probiotic activity according to complex scientific in vitro selection criteria, is presented in this work. Accurate taxonomic identification of L. acidophilus M92 has been done by PCR method. The presence of S-layer proteins, hydrophobycity, autoaggregation, coaggregation and adhesion of L. acidophilus M92 to porcine ileal epithelial cells were determined. Because the concept of synbiotic became a part of probiotic concept in last few years, it was also investigated the ability of L. acidophilus M92 to assimilate the various kinds of prebiotic substrates (sorbitol, mannitol, lactulose, raffinose, oligofructose and inulin). The combination of probiotics and non-digestible carbohydrates (prebiotics) may be a way of stabilisation and/or improvement of the probiotic effect. Such synbiotics indicate a realistic way of using biological preparationsin the prevention of gastrointestinal diseases in humans and animals.http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4029probioticsprebioticssynbioticsLactobacillus acidophilusadhesion ability
spellingShingle Jagoda Šušković
Blaženka Kos
Jadranka Frece
Sunčica Beluhan
Srećko Matošić
Synbiotic properties of Lactobacillus acidophilus M92
Mljekarstvo
probiotics
prebiotics
synbiotics
Lactobacillus acidophilus
adhesion ability
title Synbiotic properties of Lactobacillus acidophilus M92
title_full Synbiotic properties of Lactobacillus acidophilus M92
title_fullStr Synbiotic properties of Lactobacillus acidophilus M92
title_full_unstemmed Synbiotic properties of Lactobacillus acidophilus M92
title_short Synbiotic properties of Lactobacillus acidophilus M92
title_sort synbiotic properties of lactobacillus acidophilus m92
topic probiotics
prebiotics
synbiotics
Lactobacillus acidophilus
adhesion ability
url http://hrcak.srce.hr/index.php?show=clanak&id_clanak_jezik=4029
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AT blazenkakos synbioticpropertiesoflactobacillusacidophilusm92
AT jadrankafrece synbioticpropertiesoflactobacillusacidophilusm92
AT suncicabeluhan synbioticpropertiesoflactobacillusacidophilusm92
AT sreckomatosic synbioticpropertiesoflactobacillusacidophilusm92