Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura
Abstract Aim IgA-producing B cells have been found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The aim of the present study was to determine whether children with HSP possess altered B-cell subsets. Methods A total of 14 children diagnosed with HSP and age- and sex-...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-09-01
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| Series: | BMC Pediatrics |
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| Online Access: | https://doi.org/10.1186/s12887-021-02873-z |
| _version_ | 1818716010940727296 |
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| author | Ning Zhang Ge Tian Yuanyuan Sun Jing Pan Wei Xu Zhe Li |
| author_facet | Ning Zhang Ge Tian Yuanyuan Sun Jing Pan Wei Xu Zhe Li |
| author_sort | Ning Zhang |
| collection | DOAJ |
| description | Abstract Aim IgA-producing B cells have been found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The aim of the present study was to determine whether children with HSP possess altered B-cell subsets. Methods A total of 14 children diagnosed with HSP and age- and sex-matched healthy controls (HCs) were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage and absolute number of B-cell subsets and Follicular helper T (Tfh) cells were determined by flow cytometry. Finally, Spearman’s correlation coefficient was used to analyse the correlation between the percentage of Tfh cells and B-cell subsets. Results We found that compared to HCs, the frequency and absolute number of total B cells were significantly higher in children with HSP, but the percentages of plasma cells and naïve B cells were significantly lower. A significantly increased percentage and absolute number of memory nonswitched B cells were found in children with HSP compared with HCs. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4+ T cells and the percentage of CD4+CXCR5+ cells were significantly increased in patients with HSP. Moreover, significantly correlations between Tfh cells and various B-cell subsets were observed. Conclusions Our study showed a Tfh-cell-associated altered B cell compartment in children with HSP. |
| first_indexed | 2024-12-17T19:12:28Z |
| format | Article |
| id | doaj.art-b79a1a8fdbd044afa7e8e34c57fa775b |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-12-17T19:12:28Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-b79a1a8fdbd044afa7e8e34c57fa775b2022-12-21T21:35:50ZengBMCBMC Pediatrics1471-24312021-09-012111910.1186/s12887-021-02873-zAltered B cell compartment associated with Tfh cells in children with Henoch-Schonlein PurpuraNing Zhang0Ge Tian1Yuanyuan Sun2Jing Pan3Wei Xu4Zhe Li5Department of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityDepartment of Hematology, The First Affiliated Hospital of Jinzhou Medical UniversityAbstract Aim IgA-producing B cells have been found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The aim of the present study was to determine whether children with HSP possess altered B-cell subsets. Methods A total of 14 children diagnosed with HSP and age- and sex-matched healthy controls (HCs) were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage and absolute number of B-cell subsets and Follicular helper T (Tfh) cells were determined by flow cytometry. Finally, Spearman’s correlation coefficient was used to analyse the correlation between the percentage of Tfh cells and B-cell subsets. Results We found that compared to HCs, the frequency and absolute number of total B cells were significantly higher in children with HSP, but the percentages of plasma cells and naïve B cells were significantly lower. A significantly increased percentage and absolute number of memory nonswitched B cells were found in children with HSP compared with HCs. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4+ T cells and the percentage of CD4+CXCR5+ cells were significantly increased in patients with HSP. Moreover, significantly correlations between Tfh cells and various B-cell subsets were observed. Conclusions Our study showed a Tfh-cell-associated altered B cell compartment in children with HSP.https://doi.org/10.1186/s12887-021-02873-zHenoch-Schonlein purpuraB cellsCXCR5subsetsTfh |
| spellingShingle | Ning Zhang Ge Tian Yuanyuan Sun Jing Pan Wei Xu Zhe Li Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura BMC Pediatrics Henoch-Schonlein purpura B cells CXCR5 subsets Tfh |
| title | Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura |
| title_full | Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura |
| title_fullStr | Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura |
| title_full_unstemmed | Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura |
| title_short | Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura |
| title_sort | altered b cell compartment associated with tfh cells in children with henoch schonlein purpura |
| topic | Henoch-Schonlein purpura B cells CXCR5 subsets Tfh |
| url | https://doi.org/10.1186/s12887-021-02873-z |
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