Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection
The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fl...
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| Format: | Article |
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MDPI AG
2018-04-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/18/5/1309 |
| _version_ | 1828393738262544384 |
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| author | Julia Metzner Katrin Luckert Karin Lemuth Martin Hämmerle Ralf Moos |
| author_facet | Julia Metzner Katrin Luckert Karin Lemuth Martin Hämmerle Ralf Moos |
| author_sort | Julia Metzner |
| collection | DOAJ |
| description | The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out. |
| first_indexed | 2024-12-10T07:43:46Z |
| format | Article |
| id | doaj.art-b79e951dda5e417eb16fb44e4b9b53a3 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-12-10T07:43:46Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-b79e951dda5e417eb16fb44e4b9b53a32022-12-22T01:57:14ZengMDPI AGSensors1424-82202018-04-01185130910.3390/s18051309s18051309Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine DetectionJulia Metzner0Katrin Luckert1Karin Lemuth2Martin Hämmerle3Ralf Moos4Robert Bosch GmbH, Corporate Research, Robert-Bosch-Campus 1, D-71272 Renningen, GermanyRobert Bosch GmbH, Corporate Research, Robert-Bosch-Campus 1, D-71272 Renningen, GermanyRobert Bosch GmbH, Corporate Research, Robert-Bosch-Campus 1, D-71272 Renningen, GermanyDepartment of Functional Materials, University of Bayreuth, Universitätsstraße 30, D-95440 Bayreuth, GermanyDepartment of Functional Materials, University of Bayreuth, Universitätsstraße 30, D-95440 Bayreuth, GermanyThe cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out.http://www.mdpi.com/1424-8220/18/5/1309cytokineselectrochemical immunosensorfluidic systemtemperature control |
| spellingShingle | Julia Metzner Katrin Luckert Karin Lemuth Martin Hämmerle Ralf Moos Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection Sensors cytokines electrochemical immunosensor fluidic system temperature control |
| title | Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection |
| title_full | Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection |
| title_fullStr | Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection |
| title_full_unstemmed | Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection |
| title_short | Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection |
| title_sort | towards an electrochemical immunosensor system with temperature control for cytokine detection |
| topic | cytokines electrochemical immunosensor fluidic system temperature control |
| url | http://www.mdpi.com/1424-8220/18/5/1309 |
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