The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test
Objective(s): Systemic and intracerebroventricular (ICV) injection of insulin possess analgesic effects. The raphe magnus nucleus (RMN) is part of the endogenous analgesia system. The objective of the present study was to evaluate the effects of ICV injection of insulin on the levels of monoamines a...
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Mashhad University of Medical Sciences
2019-08-01
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Series: | Iranian Journal of Basic Medical Sciences |
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Online Access: | http://ijbms.mums.ac.ir/article_13168_dc8f6c6229eb4b7443e1f1a2e03d5c42.pdf |
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author | Shima Balali Dehkordi Javad Sajedianfard Ali Akbar Owji |
author_facet | Shima Balali Dehkordi Javad Sajedianfard Ali Akbar Owji |
author_sort | Shima Balali Dehkordi |
collection | DOAJ |
description | Objective(s): Systemic and intracerebroventricular (ICV) injection of insulin possess analgesic effects. The raphe magnus nucleus (RMN) is part of the endogenous analgesia system. The objective of the present study was to evaluate the effects of ICV injection of insulin on the levels of monoamines and their related metabolites in the RMN during the formalin test in non-diabetic and short-term diabetic rats.Materials and Methods: Sixty four adult male rats were used. Diabetes was induced by Streptozotocin (STZ) (60 mg/kg, IP); insulin (5 mU/animal, 5 μl) was injected into the left ventricle. Microdialysis was performed in each rat. Samples were collected at 15 min intervals. After taking the base sample of microdialysis, 50 μl of 2.5% formalin was injected into the plantar surface of the hind paw, and the level of nociception was recorded every 15 sec for 1 hr. Monoamines and their metabolites concentrations were measured using the HPLC-ECD method.Results: Findings showed that ICV injection of insulin in non-diabetic rats increased the concentration of monoamines and their related metabolites in the RMN. In diabetic rats, injection of insulin decreased the concentrations of monoamines and their related metabolites in the RMN (P |
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issn | 2008-3866 2008-3874 |
language | English |
last_indexed | 2024-12-22T11:38:23Z |
publishDate | 2019-08-01 |
publisher | Mashhad University of Medical Sciences |
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series | Iranian Journal of Basic Medical Sciences |
spelling | doaj.art-b7a26038d9314f92b75b3924cd11ce7b2022-12-21T18:27:22ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742019-08-0122891592110.22038/ijbms.2019.35580.848513168The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin testShima Balali Dehkordi0Javad Sajedianfard1Ali Akbar Owji2Department of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Basic Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranObjective(s): Systemic and intracerebroventricular (ICV) injection of insulin possess analgesic effects. The raphe magnus nucleus (RMN) is part of the endogenous analgesia system. The objective of the present study was to evaluate the effects of ICV injection of insulin on the levels of monoamines and their related metabolites in the RMN during the formalin test in non-diabetic and short-term diabetic rats.Materials and Methods: Sixty four adult male rats were used. Diabetes was induced by Streptozotocin (STZ) (60 mg/kg, IP); insulin (5 mU/animal, 5 μl) was injected into the left ventricle. Microdialysis was performed in each rat. Samples were collected at 15 min intervals. After taking the base sample of microdialysis, 50 μl of 2.5% formalin was injected into the plantar surface of the hind paw, and the level of nociception was recorded every 15 sec for 1 hr. Monoamines and their metabolites concentrations were measured using the HPLC-ECD method.Results: Findings showed that ICV injection of insulin in non-diabetic rats increased the concentration of monoamines and their related metabolites in the RMN. In diabetic rats, injection of insulin decreased the concentrations of monoamines and their related metabolites in the RMN (Phttp://ijbms.mums.ac.ir/article_13168_dc8f6c6229eb4b7443e1f1a2e03d5c42.pdfFormalin testInsulinMicrodialysisMonoaminesPain |
spellingShingle | Shima Balali Dehkordi Javad Sajedianfard Ali Akbar Owji The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test Iranian Journal of Basic Medical Sciences Formalin test Insulin Microdialysis Monoamines Pain |
title | The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test |
title_full | The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test |
title_fullStr | The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test |
title_full_unstemmed | The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test |
title_short | The effect of intra-cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non-diabetic and short-term diabetic rats in the formalin test |
title_sort | effect of intra cerebroventricular injection of insulin on the levels of monoamines on the raphe magnus nucleus of non diabetic and short term diabetic rats in the formalin test |
topic | Formalin test Insulin Microdialysis Monoamines Pain |
url | http://ijbms.mums.ac.ir/article_13168_dc8f6c6229eb4b7443e1f1a2e03d5c42.pdf |
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