Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates
Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open questio...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-01-01
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| Series: | Stem Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671115003707 |
| _version_ | 1818050956078612480 |
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| author | Mattia Quattrocelli Giorgia Giacomazzi Sarah Y. Broeckx Liesbeth Ceelen Selin Bolca Jan H. Spaas Maurilio Sampaolesi |
| author_facet | Mattia Quattrocelli Giorgia Giacomazzi Sarah Y. Broeckx Liesbeth Ceelen Selin Bolca Jan H. Spaas Maurilio Sampaolesi |
| author_sort | Mattia Quattrocelli |
| collection | DOAJ |
| description | Induced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage. |
| first_indexed | 2024-12-10T11:01:42Z |
| format | Article |
| id | doaj.art-b7a5f0dfd1f64901a4700997b72e45b1 |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-10T11:01:42Z |
| publishDate | 2016-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-b7a5f0dfd1f64901a4700997b72e45b12022-12-22T01:51:40ZengElsevierStem Cell Reports2213-67112016-01-0161556310.1016/j.stemcr.2015.12.005Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic FatesMattia Quattrocelli0Giorgia Giacomazzi1Sarah Y. Broeckx2Liesbeth Ceelen3Selin Bolca4Jan H. Spaas5Maurilio Sampaolesi6Translational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, BelgiumTranslational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, BelgiumGlobal Stem Cell Technology, ANACURA Group, 9940 Evergem, BelgiumPathlicon, ANACURA Group, 9940 Evergem, BelgiumPathlicon, ANACURA Group, 9940 Evergem, BelgiumGlobal Stem Cell Technology, ANACURA Group, 9940 Evergem, BelgiumTranslational Cardiomyology Lab, Stem Cell Biology and Embryology Unit, Department Development and Regeneration, KU Leuven, 3000 Leuven, BelgiumInduced pluripotent stem cells (iPSCs) hold great potential not only for human but also for veterinary purposes. The equine industry must often deal with health issues concerning muscle and cartilage, where comprehensive regenerative strategies are still missing. In this regard, a still open question is whether equine iPSCs differentiate toward muscle and cartilage, and whether donor cell type influences their differentiation potential. We addressed these questions through an isogenic system of equine iPSCs obtained from myogenic mesoangioblasts (MAB-iPSCs) and chondrogenic mesenchymal stem cells (MSC-iPSCs). Despite similar levels of pluripotency characteristics, the myogenic differentiation appeared enhanced in MAB-iPSCs. Conversely, the chondrogenic differentiation was augmented in MSC-iPSCs through both teratoma and in vitro differentiation assays. Thus, our data suggest that equine iPSCs can differentiate toward the myogenic and chondrogenic lineages, and can present a skewed differentiation potential in favor of the source cell lineage.http://www.sciencedirect.com/science/article/pii/S2213671115003707equine stem cellsmyogenic differentiationchondrogenic differentiationequine iPSCsintrinsic lineage propensity |
| spellingShingle | Mattia Quattrocelli Giorgia Giacomazzi Sarah Y. Broeckx Liesbeth Ceelen Selin Bolca Jan H. Spaas Maurilio Sampaolesi Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates Stem Cell Reports equine stem cells myogenic differentiation chondrogenic differentiation equine iPSCs intrinsic lineage propensity |
| title | Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates |
| title_full | Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates |
| title_fullStr | Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates |
| title_full_unstemmed | Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates |
| title_short | Equine-Induced Pluripotent Stem Cells Retain Lineage Commitment Toward Myogenic and Chondrogenic Fates |
| title_sort | equine induced pluripotent stem cells retain lineage commitment toward myogenic and chondrogenic fates |
| topic | equine stem cells myogenic differentiation chondrogenic differentiation equine iPSCs intrinsic lineage propensity |
| url | http://www.sciencedirect.com/science/article/pii/S2213671115003707 |
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